Effect of SMAC Gene on Sensitivity of Lung Adenocarcinoma Cells to Paclitaxel and Cell Viability Based on caspase-3/Bcl-2/Bax Signaling Pathway

CHEN Kang; CHEN Ying; NIU Zongxin; KANG Li; ZULIPEYA·Aibaidula

doi:10.3971/j.issn.1000-8578.2023.22.1114

Cancer Research on Prevention and Treatment > 2023 > 50(4): 357-363. > DOI: 10.3971/j.issn.1000-8578.2023.22.1114

CHEN Kang, CHEN Ying, NIU Zongxin, KANG Li, ZULIPEYA·Aibaidula. Effect of SMAC Gene on Sensitivity of Lung Adenocarcinoma Cells to Paclitaxel and Cell Viability Based on caspase-3/Bcl-2/Bax Signaling Pathway[J]. Cancer Research on Prevention and Treatment, 2023, 50(4): 357-363. DOI: 10.3971/j.issn.1000-8578.2023.22.1114

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Effect of SMAC Gene on Sensitivity of Lung Adenocarcinoma Cells to Paclitaxel and Cell Viability Based on caspase-3/Bcl-2/Bax Signaling Pathway

Department of Thoracic Surgery, Xinjiang Uygur Autonomous Region People's Hospital, Urumchi 830000, China

Funding:

Natural Science Foundation of Xinjiang Uygur Autonomous Region 2017D01C136

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Corresponding author:
CHEN Ying, E-mail: 1521746909@qq.com
Received Date: September 25, 2022
Revised Date: October 13, 2022
Available Online: January 12, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the effect of the SMAC gene on paclitaxel sensitivity and cellular activity in lung adenocarcinoma cells based on the caspase-3/Bcl-2/Bax signaling pathway.
Methods
A paclitaxel-resistant cell line A549/Taxol was established for lung adenocarcinoma, and the cells were divided into four following groups: pcDNA-NC (transfected with pcDNA-NC blank vector), pcDNA-SMAC (transfected with pcDNA-SMAC vector), siRNA-NC (transfected with siRNA-NC empty virus vector), and siRNA-SMAC groups (transfected with siRNA-SMAC lentiviral vector). The SMAC mRNA expression in cells was detected by qRT-PCR; cell sensitivity was detected by MTT; cell proliferation ability was detected by cloning assay; cell invasion ability was detected by Transwell; apoptosis ability was detected by flow cytometry assay; and caspase-3, Bcl-2 and Bax protein expression in cells were detected by Western blot analysis.
Results
The SMAC mRNA expression was significantly lower in A549 cells compared with BEAS-2B cells (P < 0.05). The SMAC mRNA expression was significantly higher in the pcDNA-SMAC group than that in the pcDNA-NC group cells (P < 0.05). The SMAC mRNA expression was significantly lower in the cells of the siRNA-SMAC group (P < 0.05) than that in the siRNA-NC group. The SMAC mRNA expression was significantly lower in the cells of the siRNA-SMAC group (P < 0.05) than in the siRNA-NC group. Compared with the pcDNA-NC group, the cell IC₅₀, cell clone number, cell invasion ability, and Bcl-2 protein and Bcl-2/Bax ratio were significantly lower in the pcDNA-SMAC group, the cell resistance index reversal was 2.51-fold, and the apoptosis ability and caspase-3, as well as Bax protein expression, were significantly higher (P < 0.05). Compared with the siRNA-NC group, cell IC₅₀, cell clone number, cell invasion ability, and Bcl-2 protein and Bcl-2/Bax ratio were significantly higher in the siRNA-SMAC group, and apoptosis ability and caspase-3 and Bax protein expression were significantly lower (P < 0.05).
Conclusion
High expression of SMAC increases paclitaxel sensitivity, inhibits cell growth and invasion, promotes apoptosis in lung adenocarcinoma cells, and has a regulatory effect on the caspase-3/Bcl-2/Bax signaling pathway.
- caspase-3/Bcl-2/Bax signaling pathway,
- SMAC gene,
- Lung adenocarcinoma cells,
- Paclitaxel resistance sensitivity,
- Proliferation,
- Invasion,
- Apoptosis

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Competing interests: The authors declare that they have no competing interests.

References (30)

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Effect of SMAC Gene on Sensitivity of Lung Adenocarcinoma Cells to Paclitaxel and Cell Viability Based on caspase-3/Bcl-2/Bax Signaling Pathway