Effect and Mechanism of RBM8A on Proliferation, Migration and Apoptosis of Human Endometrial Cancer HEC-1A Cells

TAN Dongmei; ZHANG Jingjing; SHI Yimin; HAN Sai; GENG Wei; SUN Jianyi; WANG Yayu; ZHANG Xiurong

doi:10.3971/j.issn.1000-8578.2023.22.0705

Cancer Research on Prevention and Treatment > 2023 > 50(1): 27-32. > DOI: 10.3971/j.issn.1000-8578.2023.22.0705

TAN Dongmei, ZHANG Jingjing, SHI Yimin, HAN Sai, GENG Wei, SUN Jianyi, WANG Yayu, ZHANG Xiurong. Effect and Mechanism of RBM8A on Proliferation, Migration and Apoptosis of Human Endometrial Cancer HEC-1A Cells[J]. Cancer Research on Prevention and Treatment, 2023, 50(1): 27-32. DOI: 10.3971/j.issn.1000-8578.2023.22.0705

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Effect and Mechanism of RBM8A on Proliferation, Migration and Apoptosis of Human Endometrial Cancer HEC-1A Cells

1.
TCM Gynecology, Shandong Provincial Maternal and Child Health Care Hospital, Ji'nan 250000, China
2.
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Ji'nan 250000, China

Funding:

Traditional Chinese Medicine Science and Technology Project of Shandong Province 2021M202

More Information

Corresponding author:
ZHANG Xiurong, E-mail: 18615585827@163.com
Received Date: June 21, 2022
Revised Date: October 30, 2022
Available Online: January 12, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the effect of silenced RBM8A gene on the biological behavior (proliferation, migration, and apoptosis) of human endometrial cancer HEC-1A cells and its possible mechanism.
Methods
The hairpin shRNA targeted by the RBM8A gene was designed, and the best shRNA silencing fragment was screened. The recombinant lentiviral interference vector carrying the target gene was constructed and used to infect HEC-1A cells. Cells with stable knockdown of RBM8A gene were screened by puromycin as the experimental group (shRBM8A), while the shRNA of nonsense sequence was designed as the control group (shControl). CCK-8 method was used to detect cell proliferation, and flow cytometry was used to detect cell apoptosis. Transwell assay was used to detect cell migration and invasion. Western blot was used to analyze the expression of apoptosis-related proteins and EMT signal transduction pathway related proteins.
Results
In comparison with the shControl group, after RBM8A knockdown, HEC-1A cell proliferation was reduced, apoptosis was increased, migration and invasion ability were significantly inhibited (P < 0.05), the expression of apoptosis-related proteins cleaved caspase 9 and caspase 3 increased, EMT-related protein E-cadherin expression increased, and Vimentin expression decreased.
Conclusion
RBM8A gene silencing can inhibit the proliferation, migration, and invasion and promote the apoptosis of endometrial cancer cells. The inhibition of EMT signal transduction pathway may be its mechanism.
- Human endometrial cancer,
- RBM8A,
- Proliferation,
- Migration,
- Apoptosis,
- Epithelial-mesenchymal transition

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Competing interests: The authors declare that they have no competing interests.

References (32)

References

[1]	Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2): 115-132. doi: 10.3322/caac.21338
[2]	Solmaz U, Mat E, Dereli ML, et al. Stage-Ⅲ and -Ⅳ endometrial cancer: A single oncology centre review of 104 cases[J]. J Obstet Gynaecol, 2016, 36(1): 81-86. doi: 10.3109/01443615.2015.1041890
[3]	Soumerai TE, Donoghue MTA, Bandlamudi C, et al. Clinical Utility of Prospective Molecular Characterization in Advanced Endometrial Cancer[J]. Clin Cancer Res, 2018, 24(23): 5939-5947. doi: 10.1158/1078-0432.CCR-18-0412
[4]	Boisramé A, Devillers H, Onésime D, et al. Exon junction complex components Y14 and Mago still play a role in budding yeast[J]. Sci Rep, 2019, 9(1): 849. doi: 10.1038/s41598-018-36785-3
[5]	van Weelden WJ, Lalisang RI, Bulten J, et al. Impact of hormonal biomarkers on response to hormonal therapy in advanced and recurrent endometrial cancer[J]. Am J Obstet Gynecol, 2021, 225(4): 407. e1-407. e16. doi: 10.1016/j.ajog.2021.05.007
[6]	Ho CH, Paolantoni C, Bawankar P, et al. Treisman: An exon junction complex-independent function of Barentsz in neuromuscular synapse growth[J]. EMBO Rep, 2022, 23(1): e53231.
[7]	Le Hir H, Saulière J, Wang Z. The exon junction complex as a node of post-transcriptional networks[J]. Nat Rev Mol Cell Biol, 2016, 17(1): 41-54. doi: 10.1038/nrm.2015.7
[8]	McSweeney C, Dong F. Full function of exon junction complex factor, Rbm8a, is critical for interneuron development[J]. Transl Psychiatry, 2020, 10(1): 379. doi: 10.1038/s41398-020-01065-0
[9]	Salicioni AM, Xi M, Vanderveer LA, et al. Identification and structural analysis of human RBM8A and RBM8B: two highly conserved RNA-binding motif proteins that interact with OVCA1, a candidate tumor suppressor[J]. Genomics, 2000, 69(1): 54-62. doi: 10.1006/geno.2000.6315
[10]	Wei L, Zou C, Chen L, et al. Molecular Insights and Prognosis Associated With RBM8A in Glioblastoma[J]. Front Mol Biosci, 2022, 9: 876603. doi: 10.3389/fmolb.2022.876603
[11]	Lin Y, Wei L, Hu B, et al. RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway[J]. Front Oncol, 2021, 11: 736941. doi: 10.3389/fonc.2021.736941
[12]	Liang R, Zhang J, Liu Z, et al. Mechanism and Molecular Network of RBM8A-Mediated Regulation of Oxaliplatin Resistance in Hepatocellular Carcinoma[J]. Front Oncol, 2020, 10: 585452.
[13]	Liang R, Lin Y, Ye JZ, et al. High expression of RBM8A predicts poor patient prognosis and promotes tumor progression in hepatocellular carcinoma[J]. Oncol Rep, 2017, 37(4): 2167-2176. doi: 10.3892/or.2017.5457
[14]	Lu KH, Broaddus RR. Endometrial Cancer[J]. N Engl J Med, 2020, 383(21): 2053-2064. doi: 10.1056/NEJMra1514010
[15]	Cancer Genome Atlas Research Network, Kandoth C, Schultz N, et al. Integrated genomic characterization of endometrial carcinoma[J]. Nature, 2013, 497(7447): 67-73. doi: 10.1038/nature12113
[16]	Liu Y, Patel L, Mills GB, et al. Clinical significance of CTNNB1 mutation and Wnt pathway activation in endometrioid endometrial carcinoma[J]. J Nat Cancer Inst, 2014, 106(9): dju245.
[17]	Cherniack AD, Shen H, Walter V, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma[J]. Cancer Cell, 2017, 31: 411-423. doi: 10.1016/j.ccell.2017.02.010
[18]	Supek F, Lehner B, Lindeboom RGH. Nonsense-Mediated mRNA Decay in Cancer and Other Genetic Diseases[J]. Trends Genet, 2021, 37(7): 657-668. doi: 10.1016/j.tig.2020.11.002
[19]	Popp MW, Maquat LE. Organizing principles of mammalian nonsense-mediated mRNA decay[J]. Annu Rev Genet, 2013, 47: 139-165. doi: 10.1146/annurev-genet-111212-133424
[20]	Zou D, Li R, Huang X, et al. Identification of molecular correlations of RBM8A with autophagy in Alzheimer's disease[J]. Aging(Albany NY), 2019, 11(23): 11673-11685.
[21]	Liu C, Karam R, Zhou Y, et al. The UPF1 RNA surveillance gene is commonly mutated in pancreatic adenosquamous carcinoma[J]. Nat Med, 2014, 20(6): 596-598. doi: 10.1038/nm.3548
[22]	Li F, Yi Y, Miao Y, et al. N6-Methyladenosine Modulates Nonsense-Mediated mRNA Decay in Human Glioblastoma[J]. Cancer Res, 2019, 79(22): 5785-5798. doi: 10.1158/0008-5472.CAN-18-2868
[23]	Martin L, Grigoryan A, Wang D, et al. Identification and characterization of small molecules that inhibit nonsense-mediated RNA decay and suppress nonsense p53 mutations[J]. Cancer Res, 2014, 74(11): 3104-3113. doi: 10.1158/0008-5472.CAN-13-2235
[24]	Pastor F, Kolonias D, Giangrande PH, et al. Induction of tumour immunity by targeted inhibition of nonsense-mediated mRNA decay[J]. Nature, 2010, 465(7295): 227-230. doi: 10.1038/nature08999
[25]	Dvinge H, Kim E, Abdel-Wahab O, et al. RNA splicing factors as oncoproteins and tumour suppressors[J]. Nat Rev Cancer, 2016, 16(7): 413-430. doi: 10.1038/nrc.2016.51
[26]	Ishigaki Y, Nakamura Y, Tatsuno T, et al. Depletion of RNA-binding protein RBM8A (Y14) causes cell cycle deficiency and apoptosis in human cells[J]. Exp Biol Med (Maywood), 2013, 238(8): 889-897. doi: 10.1177/1535370213494646
[27]	Zou D, McSweeney C, Sebastian A, et al. A critical role of RBM8a in proliferation and differentiation of embryonic neural progenitors[J]. Neural Dev, 2015, 10: 18. doi: 10.1186/s13064-015-0045-7
[28]	Yang P, Ruan Y, Yan Z, et al. Comprehensive analysis of lymph nodes metastasis associated genes in cervical cancer and its significance in treatment and prognosis[J]. BMC Cancer, 2021, 21(1): 1230. doi: 10.1186/s12885-021-08945-8
[29]	Lin Y, Liang R, Qiu Y, et al. Expression and gene regulation network of RBM8A in hepatocellular carcinoma based on data mining[J]. Aging (Albany), 2019, 11(2): 423-447. doi: 10.18632/aging.101749
[30]	Liu J, He Y, Li C, et al. Increased KIAA0101 gene expression associated with poor prognosis in breast cancer[J]. Transl Cancer Res, 2021, 10(9): 4009-4019. doi: 10.21037/tcr-20-3064
[31]	Muromoto R, Taira N, Ikeda O, et al. The exon-junction complex proteins, Y14 and MAGOH regulate STAT3 activation[J]. Biochem Biophy Res Commun, 2009, 382(1): 63-68. doi: 10.1016/j.bbrc.2009.02.127
[32]	Derynck R, Weinberg RA. EMT and Cancer: More Than Meets the Eye[J]. Dev Cell, 2019, 49(3): 313-316. doi: 10.1016/j.devcel.2019.04.026

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Effect and Mechanism of RBM8A on Proliferation, Migration and Apoptosis of Human Endometrial Cancer HEC-1A Cells