Objective To investigate the effect of miR-205 on the invasion and migration of HGC27 cells and related mechanism.
Methods The expression of miR-205 in four kinds of gastric cancer cell lines (AGS, MKN74, HGC27, SGC7901) were measured by real-time quantitative PCR. miR-205 mimic was transfected into HGC27 cells for up-regulation of miR-205, then cells invasion and migration were detected by scratch test and Transwell assay. Furthermore, epithelial to mesenchymal transition (EMT) progress and the activation of related signal pathway were measured by Western blot.
Results Real-time quantitative PCR showed that miR-205 was significantly down-regulated in gastric cancer cells (all P < 0.05). After miR-205 mimic transfection, miR-205 was overexpressed; the relative cellular migration rate of HGC27 cells decreased from (54.7±4.1)% to (34.1±4.5)% (P=0.005); and the cellular invasion rate decreased from (52.8±6.3)% to (32.2±4.9)% (P=0.001). Furthermore, after miR-205 was over-expressed, the expression of E-cadherin, β-catenin were up-regulated (P=0.002, P=0.003) and the expression of N-cadherin, vimentin were down-regulated (P=0.005, P=0.004) and the protein expression of Notch and snail were down-regulated (P=0.002, P=0.003).
Conclusion miR-205 is down-regulated in gastric cancer cells and it is closely related to the invasion and migration of HGC27 cells. The molecular mechanism maybe related to the inhibition of EMT progress and Notch/snail pathway.
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