Hyperoside Induces Apoptosis of Gastric Cancer Cell Line MKN-45 and Related Mechanism

Objective To investigate the effects of hyperoside(Hyp) on the proliferation and apoptosis of human gastric cell line MKN-45 and its mechanism.

Methods Gastric cells MKN-45 cultured in vitro were treated with various concentrations of hyperoside(0, 12.5, 25, 50, 100, 200μg/ml) for 24 and 48h. Cell proliferation was examined by MTT which could screen suitable drug concentration; cell cycle and apoptosis were analyzed by flow cytometry; NF-κB P65, casepase-3, Bcl-2 and Bax protein expressions were examined by Western blot.

Results Hyperoside could inhibit the growth of MKN-45 cells observably, it also blocked cell cycle at G₀/G₁ stage and induced cell apoptosis. Western blot showed that hyperoside could reduce the expression of NF-κB P65, Bcl-2 protein and elevate the expression of caspase-3, Bax protein with the increase of drug concentration.

Conclusion Hyperoside could inhibit the growth of MKN-45 cells and induce cell apoptosis. The mechanism might be related to blocking cell cycle, inhibiting NF-κB pathway, reducing the expression of NF-κB P65 and Bcl-2 protein and elevating the expression of caspase-3 and Bax protein.