Experimental Investigation on Reversing Drug Resistance of Non-small Cell Lung Cancer Stem Cells by Aqueous Extract of Taxus Chinensis

JIANG Yiqian; GUO Qingmin; MO Yongxiang; AN Suhong; XU Jialing; SU Fang; LIU Jian; YU Canbing; XU Xiaoping

doi:10.3971/j.issn.1000-8578.2016.11.003

JIANG Yiqian, GUO Qingmin, MO Yongxiang, AN Suhong, XU Jialing, SU Fang, LIU Jian, YU Canbing, XU Xiaoping. Experimental Investigation on Reversing Drug Resistance of Non-small Cell Lung Cancer Stem Cells by Aqueous Extract of Taxus Chinensis[J]. Cancer Research on Prevention and Treatment, 2016, 43(11): 933-936. DOI: 10.3971/j.issn.1000-8578.2016.11.003

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Experimental Investigation on Reversing Drug Resistance of Non-small Cell Lung Cancer Stem Cells by Aqueous Extract of Taxus Chinensis

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Objective To investigate the mechanism of aqueous extract of Taxus chinensis(AETC) reversing the drug resistance of non-small cell lung cancer stem cells.
Methods The human non-small cell lung cancer cells H460 were chosen to the experiment,and the stem cells were screened from them. The toxicity of AETC was detected with CCK8 method,and the method was also used to check AETC reversing the cisplatin resistance of H460 stem cells. FCM was used to detect the mechanism of AETC reversing the drug resistance of H460 stem cells.
Results AETC was toxiferous to H460 stem cells. The IC₅₀ of H460 stem cells was 1449.5 μg/ml. The experiment found the anti-tumor effect of cisplatin was enhanced after H460 stem cells were treated with AETC. The mechanism was related to lower drug elimination through suppressing the P-gp protein on the membrane.
Conclusion AETC could strongly reverse the drug resistance of non-small cell lung cancer stem cells.

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Experimental Investigation on Reversing Drug Resistance of Non-small Cell Lung Cancer Stem Cells by Aqueous Extract of Taxus Chinensis

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