Effect ofΔNp63αon Leukemia Inhibitory Factor Expression in Cervical Cancer Cells and Related Mechanism

Objective To explore the effect ofΔNp63αon the expression level of leukemia inhibitory factor (LIF) and its mechanism.

Methods Stable SiHa cells with overexpressedΔNp63α(SiHa/ΔNp63α) were established by stable transfection, andΔNp63αexpression was transiently silenced in ME-180 cell lines(ME-180/ΔNp63αsiRNA). The protein levels ofΔNp63α, LIF and STAT3 were detected by Western blot, while the mRNA levels ofΔNp63α, LIF, STAT3 and LncRNA ENST00000447565 were detected by qRT-PCR.

Results (1) The mRNA and protein levels of LIF and its downstream signaling factor STAT3 mRNA was significantly reduced in SiHa/ΔNp63αcells; (2) The RNA levels of LncRNA ENST00000447565 were significantly reduced in SiHa/ΔNp63αcells, ENST00000447565 and LIF were both located in chromosome 22 q12.2, within the same loci; (3) The mRNA level of LIF was significantly increased in ME-180 cells with silencedΔNp63α.

Conclusion The expressions of LIF and its downstream signaling factor STAT3 are inhibited byΔNp63αexpression, which might be associated with the down-regulation of LncRNA ENST00000447565.