Advanced Search
HU Wei, HUA Shengni, XIAO Lushan, WU Dehua. Effects of High Mobility Group Box-1 Protein on Migration and Invasion Ability of Nasopharyngeal Carcinoma Cell Line C666-1 in vitro[J]. Cancer Research on Prevention and Treatment, 2015, 42(10): 955-959. DOI: 10.3971/j.issn.1000-8578.2015.10.001
Citation: HU Wei, HUA Shengni, XIAO Lushan, WU Dehua. Effects of High Mobility Group Box-1 Protein on Migration and Invasion Ability of Nasopharyngeal Carcinoma Cell Line C666-1 in vitro[J]. Cancer Research on Prevention and Treatment, 2015, 42(10): 955-959. DOI: 10.3971/j.issn.1000-8578.2015.10.001

Effects of High Mobility Group Box-1 Protein on Migration and Invasion Ability of Nasopharyngeal Carcinoma Cell Line C666-1 in vitro

More Information
  • Received Date: February 01, 2015
  • Revised Date: July 19, 2015
  • Objective To observe the effects of high mobility group box-1 protein (HMGB1) on the migration and invasion ability of human nasopharyngeal carcinoma(NPC) cell line-C666-1 in vitro and to preliminarily explore its potential mechanism. Methods The HMGB1 expression in NPC cell line C666-1 was down-regulated by siRNA. The cell migration and invasion ability were detected by scratch test and Transwell. Invasion-related protein MMP2 and MMP9 expression in these cells were detected by Western blot. Results Compared with the control group, the cells migration and invasion ability and MMP2 expression were obviously decreased in siHMGB1 group (all P<0.001), but MMP9 expression was not changed obviously. Conclusion HMGB1 promotes the migration and invasion ability of human NPC cell line C666-1 in vitro, and the effect on invasion ability may be via regulating MMP2 expression.
  • [1]
    Wei WI, Sham JS T. Nasopharyngeal carcinoma[J]. Lancet, 2005, 36 5(94): 2041-54.
    [2]
    Tang LL, Sun Y, Mao YP, et al. Prognostic value of parapharyngeal extension in nasopharyngeal carcinoma treated with intensity modulated radiotherapy[J]. Radiother Oncol, 2014, 110(3): 404-8.
    [3]
    Mao YP, Zhou GQ, Liu LZ, et al. Comparison of radiological and clinical features of temporal lobe necrosis in nasopharyngeal carcinoma patients treated with 2D radiotherapy or intensitymodulated radiotherapy[J]. Br J Cancer, 2014, 110 (11): 2633-9.
    [4]
    Zhao CB, Bao JM, Lu YJ, et al. Co-expression of RAGE and HMGB1 is associated with cancer progression and poor patient outcome of prostate cancer[J]. Am J Cancer Res, 2014, 4(4): 36 9-77.
    [5]
    Yang GL, Zhang LH, Bo JJ, et al Increased expression of HMGB1 is associated with poor prognosis in human bladder cancer[J]. J Surg Oncol, 2012, 106(1): 57-61.
    [6]
    Qiu G, Li Y, Liu Z, et al. Clinical value of serum HMGB1 in diagnosis and prognosis of laryngeal squamous cell carcinoma[J]. Med Oncol, 2014, 31(12): 316.
    [7]
    Bald T, Quast T, Landsberg J, et al. Ultraviolet-radiationinduced inflammation promotes angiotropism and metastasis in melanoma[J]. Nature, 2014, 507 (7490): 109-13.
    [8]
    Wu D, Ding Y, Wang S, et al. Increased expression of high mobilitygroup box 1 (HMGB1) is associated with progression and poor prognosis in human nasopharyngeal carcinoma[J]. J Pathol, 2008, 21 6(2): 167-75.
    [9]
    Yang J, Zhou M, Zhao R, et al. Identification of candidate biomarkers for the early detection of nasopharyngeal carcinoma by quantitative proteomic analysis[J]. J Proteomics, 2014, 109: 16 2-75.
    [10]
    Kostova N, Zlateva S, Ugrinova I, et al. The expression of HMGB1 protein and its receptor RAGE in human malignant tumors[J]. Mol Cell Biochem, 2010, 337(1-2): 251-8.
    [11]
    Pusterla T, Nèmeth J, Stein I, et al. Receptor for advanced glycation endproducts (RAGE) is a key regulator of oval cell activation and inflammation-associated liver carcinogenesis in mice[J]. Hepatology, 2013, 58(1): 363-73.
    [12]
    Sun W, Liu DB, Li WW, et al. Interleukin-6 promotes the migration and invasion of nasopharyngeal carcinoma cell lines and upregulates the expression of MMP-2 and MMP-9[J]. Int J Oncol, 2014, 44(5): 1551-60.
    [13]
    Chien SY, Hsieh MJ, Chen CJ, et al. Nobiletin inhibits invasion and migration of human nasopharyngeal carcinoma cell lines by involving ERK1/2 and transcriptional inhibition of MMP-2[J]. Expert Opin Ther Targets, 2015, 19(3): 307-20.
    [14]
    Jeong YJ, Shin JM, Bae YS, et al. Melittin has a chondroprotective effect by inhibiting MMP-1 and MMP-8 expressions via blocking NF-κB and AP-1 signaling pathway in chondrocytes[J]. Int Immunopharmacol, 2015, 25(2): 400-5.
    [15]
    Hu A, Huang JJ, Jin XJ, et al. Curcumin suppresses invasiveness and vasculogenic mimicry of squamous cell carcinoma of the larynx through the inhibition of JAK-2/STAT-3 signaling pathway[J]. Am J Cancer Res, 2014, 5(1): 278-88.
    [16]
    Wagner KU, Schmidt JW. The two faces of Janus kinases and their respective STATs in mammary gland development and cancer[J]. Carcinog, 2011, 10: 32.
    [17]
    Slattery ML, Lundgreen A, Kadlubar SA, et al. JAK/STAT/SOCSsignaling pathway and colon and rectal cancer[J]. Mol Carcinog, 20 13, 52(2):155-66.
    [18]
    Liu H, Yao YM, Yu Y, et al. Role of Janus kinase/signal transducer and activator of transcription pathway in regulation of expression and inflammation-promoting activity of high mobility group box protein 1 in rat peritoneal macrophages[J]. Shock, 2007, 27 (1): 55 -60.
    [19]
    Feng XJ, Liu SX, Wu C, et al. The PTEN/PI3K/Akt signaling pathway mediates HMGB1-induced cell proliferation by regulating the NF-kappaB/cyclin D1 pathway in mouse mesangial cells[J]. Am J Physiol Cell Physiol, 2014, 306(12): C1119-28.
    [20]
    Xu X, Zhu H, Wang T, et al. Exogenous high-mobility group box 1 inhibits apoptosis and promotes the proliferation of lewis cells via RAGE/TLR4-dependent signal pathways[J]. Scand J Immunol, 20 14, 79(6): 386-94.
    [21]
    Agalave NM, Larsson M, Abdelmoaty S, et al. Spinal HMGB1 induces TLR4-mediated long-lasting hypersensitivity and glial activation and regulates pain-like behavior in experimental arthritis[J]. Pain, 2014, 155 (9): 1802-13.
  • Related Articles

    [1]SHAN Wenhao, YI Weijiang, ZHANG Sijun, JIANG Wei, XIA Jian. ROCK2 Regulates Invasion and Migration of Osteosarcoma Cells[J]. Cancer Research on Prevention and Treatment, 2022, 49(2): 95-100. DOI: 10.3971/j.issn.1000-8578.2022.21.0562
    [2]SHU Jiawu, LI Xijun, ZHU Liqin, WANG Yanyan. LncRNA WT1-AS Knockdown Promotes Invasion and Migration of Triple Negative Breast Cancer MDA-MB-231 Cells[J]. Cancer Research on Prevention and Treatment, 2021, 48(3): 244-247. DOI: 10.3971/j.issn.1000-8578.2021.20.0744
    [3]YU Jie, YANG Feng, DENG Jian, HU Linwang. Knockdown of DCLK1 Inhibits Proliferation, Migration and Invasion of Glioma Cells[J]. Cancer Research on Prevention and Treatment, 2020, 47(9): 655-659. DOI: 10.3971/j.issn.1000-8578.2020.20.0127
    [4]MENG Lingjiao, DING Ping'an, JU Yingchao, LIU Sihua, CHANG Sheng, HAN Ruoqi, PAN Shuo, LIU Fei, GU Li'na, SANG Meixiang. Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triple-negative Breast Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2019, 46(3): 233-238. DOI: 10.3971/j.issn.1000-8578.2019.18.0855
    [5]LIU Shiqiang, FAN Lidan, WANG Hua, CHEN Xu. shRNA Interferes with Cystatin C to Inhibit Invasion and Migration of Non-small Cell Lung Cancer A549 Cells[J]. Cancer Research on Prevention and Treatment, 2018, 45(12): 954-958. DOI: 10.3971/j.issn.1000-8578.2018.18.0802
    [6]YAO Yuzhou, YI Wen, LUO Yunchun, TAN Chao. Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism[J]. Cancer Research on Prevention and Treatment, 2016, 43(11): 948-953. DOI: 10.3971/j.issn.1000-8578.2016.11.006
    [7]CHEN Yonghua, GAO Qing, LI Wenli. Effect of siRNA Targeting Migration-inducing Protein 7 Gene on Invasion and Migration of Human Gastric Cancer Cells MKN45[J]. Cancer Research on Prevention and Treatment, 2015, 42(10): 979-983. DOI: 10.3971/j.issn.1000-8578.2015.10.006
    [8]LU Fen, XIAO Guihua, XUE Jun, WANG Zhengyun. Effects of miR-96 on Invasion and Migration in Lung Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2014, 41(06): 588-592. DOI: 10.3971/j.issn.1000-8578.2014.06.017
    [9]ZHONG Yue-yuan, LIU Shi-quan, HUANG Jie-an, QIN Meng-bin, JIN Hui. Sphingosine Kinase-1 Regulates LoVo Cell Invasion and Migration via Activation of ERK1/2 Pathway[J]. Cancer Research on Prevention and Treatment, 2011, 38(08): 861-865. DOI: 10.3971/j.issn.1000-8578.2011.08.003
    [10]QIANG Ting-hui, WANG Shuan-ke, XIA Ya-yi, WANG Jing, WANG Cui-fang, ZHANG Pei-gen. Effects of Celecoxib on Migration and Invasion of Osteosarcoma MG-63 Cell Lines[J]. Cancer Research on Prevention and Treatment, 2010, 37(10): 1136-1139. DOI: 10.3971/j.issn.1000-8578.2010.10.010

Catalog

    Article views (1781) PDF downloads (600) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return