Effects of Overexpression of Exogenous FUT7 on Adhesion and Migration of Breast Cancer Cell

Objective To investigate the effect of exogenous overexpression of α1,3 FucT-Ⅶ(FUT7) on adhesion and migration of human breast cancer cell line MDA-MB-231. Methodes pEGFP-C1-FUT7 eukaryotic expression vector was constructed and transfected them into MDA-MB-231 cells. The expression level of FUT7 mRNA was examined by RT-PCR; Expression of FUT7 and catalysate sLeX protein were assayed by Western blot. Infl uence of exogenous FUT7 overexpression on adhesion and migration of MDAMB-231 cells to HUVECs was analyzed by adhesion assay and transwell assay. Results The exogenous expression levels of FUT7 mRNA，sLeX protein on cell surface were signifi cantly increased in transfected MDA-MB-231 which had enhanced abilities of adhesion and cell migration. Conclusion Overexpression of exogenous FUT7 promoted the abilities of adhesion and migration of human breast cancer cell line MDAMB-231.