Advanced Search
HAN Zhengxiang, WEI Yanhong, ZHANG Yuming, GENG Jie, ZHANG Jie, DU Xiuping. Inhibitory Effect of Celecoxib on Triple-negative Breast Cancer Stem Cells in vitro[J]. Cancer Research on Prevention and Treatment, 2013, 40(12): 1119-1122. DOI: 10.3971/j.issn.1000-8578.2013.12.003
Citation: HAN Zhengxiang, WEI Yanhong, ZHANG Yuming, GENG Jie, ZHANG Jie, DU Xiuping. Inhibitory Effect of Celecoxib on Triple-negative Breast Cancer Stem Cells in vitro[J]. Cancer Research on Prevention and Treatment, 2013, 40(12): 1119-1122. DOI: 10.3971/j.issn.1000-8578.2013.12.003
shu

Inhibitory Effect of Celecoxib on Triple-negative Breast Cancer Stem Cells in vitro

  • Department of Oncology, The Affi liated Hospital of Xuzhou Medical College, Xuzhou 221002, China

More Information
  • Received Date: August 15, 2013
  • Revised Date: September 10, 2013
    Abstract
  • Objective To study the effect of selective COX-2 inhibitor celecoxib on the proliferation, apoptosis, invasion and adhesion of triple negative breast cancer stem cells, and its mechanism. Methods CCK-8 method was used to detect cell activity. AV-PI double staining method was used to identify cell apoptosis. Cell adhesion experiment was used to test the cell adhesion after treatment. Transwell invasion assay was used to detect the inhibitory effects of celecoxib on stem cell invasiveness. Results Celecoxib could signifi cantly induce the apoptosis of triple- negative breast cancer stem cells(P<0.05). The differences among different concentration groups, and different times at the same concentration were statistically signifi cant. Compared with the control group, the adhesion to Matrigel gel in treated groups was signifi cantly suppressed,and the number of invasive cells in treated groups was remarkably decreased in Transwell invasion assay(P<0.05). Conclusion Selective COX-2 inhibitor celecoxib could inhibit the proliferation, adhesion and invasiveness and induce the apoptosis of triple-negative breast cancer stem cells in a dose- and timedependent manner in vitro.
    • FullText(HTML)
    • References (6)
  • [1]
    Gierach GL, Burke A, Anderson WF. Epidemiology of triple negative breast cancers[J]. Breast Dis,2010,32(1-2):5-24.
    [2]
    Gelmon K, Dent R, Mackey JR, et al. Targeting triple-negative breast cancer: optimising therapeutic outcomes[J]. Ann Oncol,201 2, 23(9):2223-34.
    [3]
    Xie GZ, Zhan JF, Sun AM, et al. Suspension sphere culture combined with irradiation for purifying breast cancer stem cells[J]. Shi Yong Yi Xue Za Zhi, 2010, 26(11): 1893-6. [谢国柱, 詹军芳, 孙爱民, 等. 辐射与悬浮球培养联合纯化乳腺癌干细胞 [J] 实用医学杂志, 2010, 26(11): 1893-6.]
    [4]
    Reya T, Morrison SJ, Clarke MF, et al. Stem cells, cancer, and cancer stem cells[J]. Nature,2001,414(6859):105-11.
    [5]
    Al-Hajj M, Wicha MS, Benito-Hernandez A, et al. Prospective identification of tumorigenic breast cancer cells[J]. Proc Natl Acad Sci U S A,2003,100(7):3983-8.
    [6]
    Jana D, Sarkar DK, Maji A, et al. Can cyclo-oxygenase-2 be a useful prognostic and risk stratification marker in breast cancer?[J]. J Indian Med Assoc,2012,110(7):429-33.
    • Related Articles

  • Related Articles

    [1]Wu Shisheng, Shao Lihua, LI Shangr, Zhang Fei, Xie Hong, Zhang Chunxiu, Liu Guiping. Correlation of MMP-9 and MMP-2 Gene SNPs with Hepatocellular Carcinoma Invasion and Metastasis[J]. Cancer Research on Prevention and Treatment, 2012, 39(06): 683-686. DOI: 10.3971/j.issn.1000-8578.2012.06.017
    [2]Chen Shouren, Wang Zhanxiang, Shen Shanghang, Tan Guowei, Liu Xiyao, Zhu Hongwei. miR-126 Inhibits Invasion of Human Brain Glioma Cells by Decreasing MMP-2[J]. Cancer Research on Prevention and Treatment, 2012, 39(03): 264-266. DOI: 10.3971/j.issn.1000-8578.2012.03.005
    [3]ZHANG Jun-xiang, LIU Zhang-suo, WANG Jian-jun. Expression and Significance of ANGPTL3,MMP-2 and MMP-9 in Esophageal Squamous Cell Carcinoma[J]. Cancer Research on Prevention and Treatment, 2011, 38(09): 1028-1030. DOI: 10.3971/j.issn.1000-8578.2011.09.013
    [4]XUAN Xiao-yan, LI Shan-shan, ZHENG Xian-zhao, LI Na, WANG Feng. Effect of Silencing STAT3 by siRNA on Expression of MMP-2 in Esophageal Carcinoma Cell Line EC-1[J]. Cancer Research on Prevention and Treatment, 2010, 37(04): 395-397. DOI: 10.3971/j.issn.1000-8578.2010.04.007
    [5]ZHAO Bin-bin, ZHU Yun-kui, LI Ji-dong, WANG Shu, LIU Wei. Influence of Tumor-host Cooperation in Regulating MMP-2 and MMP-9 Expression in Lung Cancer[J]. Cancer Research on Prevention and Treatment, 2009, 36(09): 737-740. DOI: 10.3971/j.issn.1000-8578.2009.09.005
    [6]WAN Zhen-ling, HU Zhong-liang, LIU Bao-an. TGFβ1 Upregulates Expression of MMP-2 and MMP-9 through ERK Signaling Pathway in Gastric Carcinoma Cells[J]. Cancer Research on Prevention and Treatment, 2009, 36(02): 91-94. DOI: 10.3971/j.issn.1000-8578.2009.02.003
    [7]LUO Hong-bo, LIU Xiu-heng, CHEN Zhimin, ZHANG De-Ling. Immunohistochemical Study of COX-2 and MMP-2 in Human Renal Cell Carcinoma[J]. Cancer Research on Prevention and Treatment, 2005, 32(06): 354-346. DOI: 10.3971/j.issn.1000-8578.2783
    [8]ZHOU Hong-yan, DIAO Lu-ming, CHEN Ying, DENG Hao, LIU Li-jiang. Relationship of the Expression of MMP-2 、MMP-9 、TIMP-1 with Lymph Node Metastasis and Survival Time in Gastric Adenocarcinoma[J]. Cancer Research on Prevention and Treatment, 2005, 32(05): 274-275. DOI: 10.3971/j.issn.1000-8578.1272
    [9]CAO Ge, SHEN Zhi-xiang, HUANG Jie-an, et al. Correlation between expression of CD44v6, MMP-2 and nm23-H1 in colorectal carcinoma and its clinicalopathological characters[J]. Cancer Research on Prevention and Treatment, 2002, 29(01): 53-56. DOI: 10.3971/j.issn.1000-8578.126
    [10]ZHAO Yue-wu, KONG Ling-fei, TANG Hua, et al. Study on Relationship between Activity of MMP-2(72KD)，MMP-9(92KD) and Invasion Metastasis of Human Hepatocellular Carcinoma[J]. Cancer Research on Prevention and Treatment, 2000, 27(05): 370-372. DOI: 10.3971/j.issn.1000-8578.3400

Catalog

    DU Xiuping

    • Department of Oncology, The Affi liated Hospital of Xuzhou Medical College, Xuzhou 221002, China

    1. On this Site
    2. On Google Scholar
    3. On PubMed
    Get Citation
    PDF
    XML
    Article views (2144) PDF downloads (625) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Download Center

    Links

    Contact Us

    Address：116 Zhuodaoquan South Road,
    Hongshan District, Wuhan（430079）

    Tel：027－87670126

    Email：zlfzyjzz@vip.163.com

    This work is licensed under a Creative Commons Attribution 3.0 License.

    Email Alert RSS
    Total visits：24642222 Visits today：27430

    Copyright © Editorial Department of Cancer Prevention Research 鄂公网安备 42011102005013号 鄂ICP备2022015867号

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return