Expression of miRNA-210 Induced by Hypoxia in U251 Cells and Effect on Metastasis

Objective To investigate the effects of hypoxia on expression of miRNA-210 and U251 cell metastasis. Methods Human glioma U251 cell line was induced by hypoxia(1% O2) for different hours. The level of Hypoxia-inducible factor 1α(HIF-1α) in U251 cells was detected by western blot and the expression of miRNA-210 was tested by real time PCR. The effects of miRNA-210 on U251 cells metastasis were tested by tumor invasion assay using Transwell. The expression of Vmp1 was tested by western blot. Results The level of HIF-1α in U251 cells were signifi cantly increased in time-dependent manner when induced for 6h, 12 h, and 24 h by hypoxia, and the expression of miRNA-210 was increased with HIF-1α level. The expression of miRNA-210 was suppressed by 2-ME2, HIF-1α inhibitor. The metastasis capability of U251 cells induced by hypoxia was increased, and was inhibited by miRNA210 inhibitor antagonist miR210. The expression of Vmp1 in U251 cells induced by hypoxia was increased and reversed by antagonist miR210. Conclusion miRNA-210 could be induced by hypoxia via HIF-1α. miRNA-210 could promote U251 cells metastasis and down-regulate Vmp1 expression .