Effect of hTERTC27 Overexpression on Prolifteration and Apoptosis of Nasopharyngeal Carcinoma C666-1 Cell Line

Objective To explore the effect of hTERTC27 overexpression on the proliferation and apoptosis of nasopharyngeal carcinoma C666-1 cell line. Methods pcDNA3-hTERTC27 was transfected into nasopharyngeal carcinoma C666-1 cells with lipofectamine.The expression of hTERTC27 and Bcl-2 were detected by Western blot.The proliferation of cells was detected by MTT assay and colony formation assay.The apoptosis of cells was detected by TUNEL assay. Results C666-1 cells expressed high level of hTERTC27 after 24h transfection.Over expression of hTERTC27 obviously suppressed the cell proliferation（P<0.01）,downregulated the colony formation rate（P<0.01）,increased the TUNEL positive cells （P<0.01），and downregulated the expression level of Bcl-2 protein（P<0.01）. Conclusion Overexpression of hTERTC27 can effectively inhibit proliferation and promote apoptosis of nasopharyngeal carcinoma cells,which is possibly due to the down regulation of Bcl-2 protein.