Advanced Search
Luo Ying, Zhu Zhengyan, Guo Hongxing, Wang Peng, Liu Hui, Du Zhi. Effect of RetroNectin on Proliferation and Biological Characteristics of CIK Cells in Hepatocellular Carcinoma (HCC) Patients[J]. Cancer Research on Prevention and Treatment, 2012, 39(09): 1056-1059. DOI: 10.3971/j.issn.1000-8578.2012.09.004
Citation: Luo Ying, Zhu Zhengyan, Guo Hongxing, Wang Peng, Liu Hui, Du Zhi. Effect of RetroNectin on Proliferation and Biological Characteristics of CIK Cells in Hepatocellular Carcinoma (HCC) Patients[J]. Cancer Research on Prevention and Treatment, 2012, 39(09): 1056-1059. DOI: 10.3971/j.issn.1000-8578.2012.09.004
shu

Effect of RetroNectin on Proliferation and Biological Characteristics of CIK Cells in Hepatocellular Carcinoma (HCC) Patients

  • 1.Tianjin Regeneration Medicine and Disease Biotherapy Engineering Research Center, Tianjin Third Central Hospital,Tianjin Key Laboratory of Artificial Cell,Tianjin Institute of Hepatobiliary Disease,Tianjin 300170,China,2.Department of Hepatobiliary

More Information
  • Received Date: September 18, 2011
  • Revised Date: December 08, 2011
    Abstract
  • Objective To observe the biological characteristics of CIK cells activated by recombinant human fibronectin(RN) and establish an effective way of cell proliferation. Methods We separated the mononuclear cells(PBMCs) from 50 ml peripheral blood from 15 HCC patients PBMCs were divided into two groups,of which were activated by RN-induced (RN+anti-CD3 mAb+IL-2) or conventional method(anti-CD3 mAb+IFN-γ+IL-2) repectively.Then we estimated cell morphology,the number,cell phenotype and cytotoxic efficiency at different time points in two groups. Results RN-induced group had a 2.0~2.8 fold higher proliferation rate,compared with conventional group,P<0.05.There was also a higher percentage of activated T cells and CD3+CD8+T cells in RN-induced group.We observed a higher inhibition rate of the CIK cells to liver cancer cell lines. Conclusion RN may replace conventional method to induce CIK cell proliferation in HCC patients.
    • FullText(HTML)
    • References (10)
  • [1]
    Wang SY,Du WL,Zhang H,et al.Biological characteristics and antitumor activity of CIK cells activated byrecombinant human fibronectin for human lung cancer cell lines in vitro[J].Zhongguo Fei Ai Za Zhi,2010,13(4):277-81.[王士勇,杜微丽,张晖,等.重组人纤维连接蛋白诱导的CIK细胞的生物学特性和对肺癌细胞株杀伤活性的体外研究[J].中国肺癌杂志,2010,13(4):277-81.]
    [2]
    Schmidt-Wolf GD,Negrin RS,Schmidt-Wolf IG.Activated T cells and cytokine-induced CD3+CD56+ killer cells[J].Ann Hematol,1997,74(2):51-6.
    [3]
    Wang J,He M,Shi W,et al.Inducible costimulator(ICOS) enhances the cytolytic activity of cytokine-inducedkiller cells against gallbladder cancer in vitro and in vivo[J].Cancer Invest,2009,27(3):244-50.
    [4]
    Wu C,Jiang J,Shi L,et al.Prospective study of chemotherapy in combination with cytokine-induced killer cellsin patients suffering from advanced non-small cell lung cancer[J].Anticancer Res,2008,28(6B):3997-4002.
    [5]
    June CH.Adoptive T cell therapy for cancer in the clinic[J].J Clin Invest,2007,117(6):1466-76.
    [6]
    Lamers CH,van Elzakker P,van Steenbergen SC,et al.Retronectin-assisted retroviral transduction of primaryhuman T lymphocytes under good manufacturing practice conditions:tissue culture bag critically determines cellyield[J].Cytotherapy,2008,10(4):406-16.
    [7]
    Mao Y,Schwarzbauer JE.Fibronectin fibrillogenesis a cell-mediated matrix assembly process[J].MatrixBiol,2005,24(6):389-99.
    [8]
    Harima A,Nakaseko C,Yokota A,et al.Fibronectin promotes cell proliferation of human pre-B cell line via itsinteractions with VLA-4 and VLA-5[J].Hematology,2008,13(4):236-43.
    [9]
    Linn YC,Lau LC,Hui KM.Generation of cytokine-induced killer cells from leukemic samples with in vitrocytotoxicity against autologous and allogeneic leukaemis blasts[J].Br J Haematol,2002,116(1):78-86.
    [10]
    Linn YC,Lau SK,Liu BH,et al.Characterization of the recognition and functional heterogeneity exhibited bycytokine-induced killer cell subsets against acute myeloid leukaemia target cell[J].Immunology,2009,126(3):423-35.
    • Related Articles

  • Related Articles

    [1]WANG Yue, ZHAO Weizhe, LI Xiaojie, NAN Mengdie, XIE Meiwen, LI Peijin, HOU Li. Traditional Chinese Medicine Combined with Chemotherapy in Prevention and Treatment of Recurrence and Metastasis of Locally Advanced Gastric Cancer After Radical Resection: A Meta-analysis[J]. Cancer Research on Prevention and Treatment, 2022, 49(9): 913-922. DOI: 10.3971/j.issn.1000-8578.2022.22.0012
    [2]LV Minhao, QIN Li, LI Juntao, GUO Xuhui, LIU Fawen, CUI Shude, ZHANG Hengwei. 原发性乳腺癌分子分型与新辅助化疗疗效及预后的相关性[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 782-788. DOI: 10.3971/j.issn.1000-8578.2015.08.007
    [3]ZHANG Yanli, ZHU Shunqin, LIU Yaling, CUI Hongjuan. 恶性黑色素瘤内科治疗研究进展[J]. Cancer Research on Prevention and Treatment, 2014, 41(01): 74-78. DOI: 10.3971/j.issn.1000-8578.2014.01.017
    [5]GE Lai-zeng, WU Shi-guang, GONG Li-peng, JIN Pei-yong, LI Tong-fei. Treatment of Advanced Gastric Carcinoma by Gastric Artery Intervention Chemoembolization Combined with Radical Cure Resection[J]. Cancer Research on Prevention and Treatment, 2007, 34(04): 300-302. DOI: 10.3971/j.issn.1000-8578.1701
    [6]SONG Jin-long, SHAO Wen-bo. Percutaneous Microwave Coagulation Combined with Transcather Hepatic Arterial Chemoembolization in the Treatment of Primary Liver Cancer with Poor Lipiodol Distribution[J]. Cancer Research on Prevention and Treatment, 2006, 33(10): 767-768. DOI: 10.3971/j.issn.1000-8578.861
    [7]QIN Xiao-guang, YU Lin, PU Qing-tian, et al, . An Clinical Observation of Control Chemical Treatment Uomiting with Acupuncture and Chinese Herbal Medicine[J]. Cancer Research on Prevention and Treatment, 2000, 27(04): 283-284. DOI: 10.3971/j.issn.1000-8578.2694
    [8]Clinical Studies on Combined Treatment for Advanced Gastric Cancer with Chinese Medicines and Chemical[J]. Cancer Research on Prevention and Treatment, 1999, 26(6): 426-427.
    [9]Sun Jianwei, . Preoperative intra-arterial lnfusion chemotherapy in the treatment of advanced grastric Cancer[J]. Cancer Research on Prevention and Treatment, 1998, 25(6): 485-486.
    [10]Peng Yimei. The Therapy to Consolidate the Terminal Alimentary canal Cancer after Impulse Treatment[J]. Cancer Research on Prevention and Treatment, 1997, 24(4): 251-252.

Catalog

    Du Zhi

    • 1.Tianjin Regeneration Medicine and Disease Biotherapy Engineering Research Center, Tianjin Third Central Hospital,Tianjin Key Laboratory of Artificial Cell,Tianjin Institute of Hepatobiliary Disease,Tianjin 300170,China,2.Department of Hepatobiliary

    1. On this Site
    2. On Google Scholar
    3. On PubMed
    Get Citation
    PDF
    XML
    Article views (3040) PDF downloads (817) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Contact Us

    Address：116 Zhuodaoquan South Road,
    Hongshan District, Wuhan（430079）

    Tel：027－87670126

    Email：zlfzyjzz@vip.163.com

    This work is licensed under a Creative Commons Attribution 3.0 License.

    Email Alert RSS
    Total visits：24639897 Visits today：25103

    Copyright © Editorial Department of Cancer Prevention Research 鄂公网安备 42011102005013号 鄂ICP备2022015867号

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return