Mechanism of Splenic Excess Augmentation in Tumor-bearing Mice

Objective To explore the mechanism of splenic excess augmentation in H22-beared mice. Methods Correlation of splenic index and cell count was analyzed.Splenic cell cycle and the variety of lymphocyte were examined by flow cytometry (FCM). Results Significant correlation was observed between splenic index and total cell number(r=1.000,P<0.01).There was no obvious diversity in splenic cell cycle of mice.The proportion of T cell,CD8+T cell and CD4+T cell increased (P<0.05).At the same time CD4+/CD8+declined.There was no marked change in the proportion of B cell. Conclusion The excess augmentation in H22 bearing mice was associated with the export of cells which was restrained after the cells entered the spleen,especially the CD8+T cell was detected.CD8+T cell was bound up with immunosuppression of spleen in H22-beared mice.