Effects of Hypoxia on FPR1 Expression and Invasiveness of A549 Cells

Objective To evaluate the effects of hypoxia on the expression of formyl peptide receptor 1(FPR1) and the invasiveness of lung adenocarcinoma cell lines A549. Methods A549 cells were exposed to either normoxia(21%O₂) or hypoxia(1%O₂) condition for 4，12 and 24 h.The expressions of FPR1 mRNA levels were measured by RT-PCR.The expressions of Annexin 1 protein and FPR1 activity were investigated by Western blot and chemotaxis assay.Cells invasiveness was assayed by matrigel transwell. Results Compared with normoxia group，the FPR1 mRNA in hypoxia group increased;moreover，Annexin 1 in culture medium supernatants of A549 cells also functional increased.Treatment of FPR1 agonist fMLP increased the mRNA level of FPR1 in A549 cells.Hypoxia significantly increased the number of A549 cells invaded across the transwell matrixgel，and FPR specific antagonist Boc2 attenuated the hypoxia-induced invasiveness of A549 cells. Conclusion The results suggest that hypoxia plays an important role in the augmentation of the FPR1 expression and its agonist production，which were involved in the invasiveness of lung cancer cells.