Egr-1 Regulates Human Hepatocelluar Carcinoma Cell Migration and Adhesion

Objective To investigate the effect of dominant negative mutation of Egr-1 expression on the adhension and invasion in the human Hepatocellular carcinoma cell line BEL-7402. Methods Adenovirus of Egr-1 with dominant negative mutation was constructed and transfected the BEL-7402 cell line.Empty adenovirus-transfected and normal BEL-7402 were used as control groups.The influences of Egr-1 on cell migration and adhesion capacity were detected by cell scratch test and adhesion test. Results Transcription function competitive inhibition-type adenovirus of Egr-1 was successfully constructed and transfected into BEL-7402 cells. Compared with control BEL-7402 cells，both migration and adhesion of Ad-dnEgr-1/BEL-7402 cells were significantly lower (P<0.01).Pseudopod structure related to cell migration and adhesion significantly reduced，and FAK phosphorylation levels decreased under hypoxic conditions. Conclusion Down-regulation of Egr-1 transcription function could inhibit the proliferation and invasive of BEL-7402 cells.