Effects of 5-Aza-2′-deoxycytidine on Apoptosis and Expression of PEG10 Gene in Human Hepato Carcinoma Cell Line HepG2

Objective To investigate the effects of demethyhfing agent 5′-Aza-2′-deoxyeytidine(5-Aza-CdR)on the expression of PEG10 gene in human hepato carcinoma cell line HepG2. Methods HepG2 cells were treated with 50.0 μmol/L 5-Aza-CdR for 24,48,72 h.Apoptosis of HepG2 induced by 5-Aza-CdR was identified by fluorescence microscopy with hoechst 33342/PI staining.RT-PCR and Western blot were applied to detect the PEG10 mRNA and protein expression levels,respectively. Results After treated with 5-Aza-CdRat for indicated time,fluorescence microscopy analysis showed that the apoptosis rate of HepG2 cells increased significantly,and the expression of PEG10 mRNA and protein in HepG2 cells were reduced in a time-dependent manner(P＜0.05). Conclusion 5-Aza-CdR can induce HepG2 cell apoptosis and reduce the expression of PEG10 gene.