Expressions and Significance of CyclinA and C-myc in Skin Scar and Skin Scar Carcinoma

ObjectiveTo study the expressions and significance of CyclinA and C-myc in skin Scar and skin Scar Carcinoma. Methods We compared pathological skin scar tissues and skin scar carcinoma tissues with normal skin tissues．The expression of CyclinA and C-myc proteins were detected by the immunohistochemical method of SP．And the expression of CyclinA mRNA was detected by in situ hybridization．All data were input into the computer and statistically analyzed with SPSS （16.0） software．Results（1） CyclinA and its mRNA showed strong positive expression in scar carcinoma tissues，negative or weakly positive expression in normal skin epidermis and pathological skin scar epithelium．Compared with normal skin group and skin scar group ，the expression (optical density and positive area) of CyclinA and its mRNA in scar carcinoma group were statistically significant （P＜0.01）．No statistic differences of their expression were measured between normal skin epidermis and pathological skin scar epithelium．（2）C-myc protein showed strong positive，positive and weakly positive expression in scar carcinoma tissues，pathological skin scar epithelium and normal skin epidermis respectively．Each of two groups was statistically different（P＜0.05）．（3）Correlated analysis showed that there was positive correlation between the expression levels of CyclinA and CyclinA mRNA， CyclinA and C-myc proteins in skin scar carcinoma tissues．Conclusion（1）The high expression of CyclinA,CyclinA mRNA and C-myc may be related to the occurrence of skin scar carcinoma．（2）The high expression of C-myc may be related to the cancerization of skin scar epithelium.（3）There was the abnormal expression of CyclinA not only on protein levels，but also on gene levels in skin scar carcinoma tissues．