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WU Gang, ZHU Fang, LI Zhen-yu, XUE Jun, PENG Gang, REN Jing-hua. Role of VEGF165 Gene in Vasculogenic Mimicry Formation of HepG2[J]. Cancer Research on Prevention and Treatment, 2010, 37(11): 1245-1248. DOI: 10.3971/j.issn.1000-8578.2010.11.009
Citation: WU Gang, ZHU Fang, LI Zhen-yu, XUE Jun, PENG Gang, REN Jing-hua. Role of VEGF165 Gene in Vasculogenic Mimicry Formation of HepG2[J]. Cancer Research on Prevention and Treatment, 2010, 37(11): 1245-1248. DOI: 10.3971/j.issn.1000-8578.2010.11.009
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Role of VEGF165 Gene in Vasculogenic Mimicry Formation of HepG2

  • Department of Oncology，The Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China

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  • Received Date: August 09, 2009
  • Revised Date: April 01, 2010
    Abstract
  • Objective To detect the role of VEGF165 gene in vasculogenic mimicry formation of HepG2. Methods Two and three-dimensional culture was wed to observed the vasculogenic mimicry formation of HepG2.Eukaryotic expression plasmids pcDNA3.0-VEGF165 and pcDNA3.0-VEGF165b were constructed and transfected into HepG2 cell by Lipofectamine2000，then cell were cultured in the three-Dimensional matrix，the expression of VEGF165 and VEGF165b were detected by RT-PCR and Western blot. Results HepG2 cells could form vasculogenic mimicry in three-Dimensional matrix.The expression of VEGF165 and VEGF165b of HepG2 cells were up-regulation after transfected with their corresponding plasmids，but the vasculogenic mimicry formation of transfected HepG2 cells has no change. Conclusion HepG2 cells could form vasculogenic mimicry in three-Dimensional matrix，but the overexpression of VEGF165 and VEGF165b gene can not significantly influence the vasculogenic mimicry formation.
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    REN Jing-hua

    • Department of Oncology，The Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China

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