Effect of Glycin-extended Gastrin on Human Gastric Cancer Cell Line SGC-7901 in Vitro

Objective To explore the effect of Glycin-extended gastrin on proliferation and anti-apoptotic effect of gastric cancer cell line SGC-7901,as well as the role of G-Gly in the development of gastric cancer． Methods Cell proliferation was detected by MTT colorimetric assay at various concentrations of G-Gly，and cell apoptotic rate was examined with flow cyometry analysis． Results G-Gly can promote cell proliferation of SGC-7901 in a dose-dependant manner within a certain extent. The concentration of maximum effect is 0.1 nmol/L.Its effects can not be inhibited by antibody of CCKBR. MMC can promote SGC-7901 cell line apoptosis significantly,but G-Gly could inhibit its apoptosis induced by MMC. Conclusion G-Gly promoted cell proliferation and inhibited cell apoptosis induced by MMC．These results suggest that G-Gly may promoted the development of gastric cancer．