Anti-angiogenic Effects of Cisplatin Combined with Ginsenoside Rg3 in Nude Mice Cervical Cancer

Objective To study the anti-angiogenic effects and anti-tumor effect of cisplatin combined with ginsenoside Rg3 in nude mice cervical cancer. Methods 28 female nude mice were randomly divided into 4 groups to receive saline (0.5ml,qd),Rg3(5mg/kg) alone,cisplatin (5mg/kg) alone and Rg3 (5mg/kg) combined with cisplatin (5mg/kg) respectively.Rg3 was given by gastrogavage once a day,for 5 weeks,cisplatin 3 was injected introperitoneally once 4 days for 5 weeks.After treatments for 5 weeks,mice were killed and the sizes of solid tumors were measured,the intratumor MVD (microvessel density) was examined by immunohistochemical staining. Results The tumor weights of treated groups were significantly lower than those of the control group,while the tumor weights of Rg3 combined with cisplatin group were the lowest.The MVD value of Rg3 group were significantly lower than that of cisplatin group and control group. Conclusion Rg3 combined with cisplatin can significantly inhibit the growth of transplanted human cervical cancer in nude mice.Rg3 can obviously reduce the intratumoral MVD by inhibiting angiogenesis of malignant tumor.