Antitumoral Effect of Low Molecular Weight Polysaccharide Isolated from Agaricus Blazei Murill on Sarcoma 180 in Mice and Its Mechanism

To observe antitumor activity and inducing apoptosis of low molecular weight polysaccharide isolated from Agaricus blazei Murill (LMPAB) on sarcoma 180 in mice. Methods The BALB/c mice inoculated in armpit with S180 cell suspension were treated by intraperitoneal injection of LMPAB 50 100 and 200 mg/（kg&#8226;d） for 7days, The mice were sacrificed after 24h and tumor tissues were weighed respectively. Tumor cell apoptosis was detected by Annexin-Ⅴ-FITC/PI (Flow cytometry). Results In LMPAB group 200 mg/（kg&#8226;d） (0.695±0.096) and Polyporus polysaccharide group (0.704±0.262) tumor weight reduced compared with the model group（1.038±0.262, P<0.01), Inhibitory rate of sarcoma 180 in mice treated with 50, 100, 200 mg/（kg&#8226;d） LMPAB was 9.5%, 23% and 32%, respectively. The apoptosis rate of sarcoma 180 cells in mice treated with 50, 100, 200 mg/（kg&#8226;d） LMPAB was (39.21±3.85) %, (32.17±5.10)% and (18.71±2.52)%, respectively, higher than that in the model group mice (10.98±1.55)%. Conclusion LMPAB significantly inhibited the growth of sarcoma 180 subcutaneously implanted in mice, possibly through inducing apoptosis of tumor cells.