Role of N F- КBin Fatty Acid Synthase Inhibitor Induced Apoptosis in Human Colonic Cancer Cells colonic cancer cells

Objective To explore the role of nuclear factor KappaB(NF-κB)in fatty acid synthase inhibitor(FASI),cerulenin,induced apoptosis in human colonic cancer cells. Methods Microculture terazolium assay(MTT),agarose gel electrophoresis,fluorescence microscope(AO/EB) and flow cytometyr(FCM)assays were performed for the effects of FASI in various concentration on proliferation and apoptosis in human colonic cancer cells in vitro; electrophoretic mobility shift assay(EMSA) was used as the determination method of N...