Proliferation of ovarian cancer cell SKOV3 were inhibited by Anti-VEGF hairpin ribozyme gene

Objectivc To observe the effect of Anti-VEGF hairpin ribozyme gene to ovarian cancer cells. Methods Anti-VEGF hairpin ribozyme gene eukaryotic expression vector was introduced into ovarian cancer SKOV3 cells by using lipofectin mediation; MTT, colony forming examination, soft agar colony forming examination and FCM were used to observe the effect of proliferation to ovarian cancer cells. Results The growth of transfected SKOV3-RZ cells were slower; The average colony forming efficiency and soft agar c...