p16 gene expression and deletionin in the esophageal carcinoma of jieyang city

Objective To study p16 gene expression and deletion in the esophageal carcinoma in the endemic area of Jieyang city. Methods The expression of the p16 protein was detected with immunohistochemistry (IHC), homozygous deletion of the p16 gene was assessed by polymerase chain reaction (PCR) and the level of the p16mRNA signal was displayed by in situ hybridization (ISH). Results (1) p16 protein was detected in 26 cases (43.3%), with lymph node metastasis expressed positive compared with 21/40(52.5%) cases w...