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QI Feng, GAO Yan-feng, SUN Zhan-qiang, HU Hong-min, CHEN Li-xiang, QI Yuan-ming. Identification and Optimization of HLA-A2 Restricted COX-2 Derived Antigen Peptides[J]. Cancer Research on Prevention and Treatment, 2008, 35(07): 460-463. DOI: 10.3971/j.issn.1000-8578.1481
Citation: QI Feng, GAO Yan-feng, SUN Zhan-qiang, HU Hong-min, CHEN Li-xiang, QI Yuan-ming. Identification and Optimization of HLA-A2 Restricted COX-2 Derived Antigen Peptides[J]. Cancer Research on Prevention and Treatment, 2008, 35(07): 460-463. DOI: 10.3971/j.issn.1000-8578.1481
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Identification and Optimization of HLA-A2 Restricted COX-2 Derived Antigen Peptides

  • Department of Bioengineering; Zhengzhou University; Zhengzhou 450001; China

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  • Corresponding author:

    QI Yuan2ming

  • Received Date: December 31, 2006
  • Revised Date: January 23, 2008
    Abstract
  • 目的 筛选和鉴定食管癌广泛高表达蛋白COX-2的HLA-A2限制性CTL表位。方法 运用生物信息学的方法，以SYFPEITHI法初步预测，结合MHCPred 2.0和NetChop3.0 Server在线分析，设计出五条新的抗原肽。通过标准Fmoc固相合成法合成抗原肽，以流式细胞仪对其与HLA-A2分子的结合力和稳定性进行分析，并对结合力较低的P66（FI<0.5）的原始序列进行改造，将其序列第一位的苯丙氨酸替换成酪氨酸（P66Y1），以MTT实验检测抗原肽诱导的特异性CTL对肿瘤细胞的杀伤作用。 结果 P321对HLA-A2分子有较高的结合力（FI＝1.93），同时P66Y1与HLA-A2分子的结合力显著增强（FI＝1.48），并且它们与HLA-A2分子的稳定性较好（DC50>2h）。P321和P66Y1对表达COX-2的EC-9706、EC-1的杀伤率分别明显高于P66。结论 源于食管癌广泛高表达抗原COX-2的抗原肽P321和P66Y1能分别有效激发HLA-A2限制性CTL的免疫应答并杀伤肿瘤细胞。
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    Corresponding author: QI Yuan-ming

    • Department of Bioengineering; Zhengzhou University; Zhengzhou 450001; China

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