Apoptosis of MCF-7 cells induced by fusion gene of Fas/RARα

Objective To test the apoptosis of MCF-7 cells after transfection of the vector of Fas/RARα fusion gene induced by ATRA. Methods After transfection of fusion gene vector pRARE-Fas/RAR alpha and treatment of RA in tumor cells, the cellular proliferation capacity was observed with MTT assay, the cell cycle distribution and the apoptotic rate were measured with flow cytometry, DNA ladder and TUNEL. Results The proliferation capacity was decreased, the apoptotic rate was increased in MCF-7 cells treated by R...