Study of Prote in and mRNA of Type Ⅰ、Ⅱ and Ⅲ Collagen in Human Osteosarcoma by Immunohistochemistry and in Situ Hybridization

ZHANG Hui-zhong; QIU Ju-shi; HAO Ya-ping; et al

doi:10.3971/j.issn.1000-8578.1358

ZHANG Hui-zhong, QIU Ju-shi, HAO Ya-ping, et al. Study of Prote in and mRNA of Type Ⅰ、Ⅱ and Ⅲ Collagen in Human Osteosarcoma by Immunohistochemistry and in Situ Hybridization[J]. Cancer Research on Prevention and Treatment, 2001, 28(05): 345-347. DOI: 10.3971/j.issn.1000-8578.1358

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Study of Prote in and mRNA of Type Ⅰ、Ⅱ and Ⅲ Collagen in Human Osteosarcoma by Immunohistochemistry and in Situ Hybridization

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Objective To investigate expression of type Ⅰ?Ⅱ and Ⅲ collagen in human osteosarcoma and their relationship with classification and differentiation of osteosarcoma. Methods The protein and mRNA of type Ⅰ?Ⅱ and Ⅲ collagen in 46 cases of osteosarcoma were detected using immunohistochemistry (LSAB) and in situ hybridization;EMAL 200 image software was used to analyse staining result. Results Besides type Ⅰ collagen (positive rate 87%,grey value 171.99±14.74),there were also expression of type Ⅱ and Ⅲ collagen i...

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Study of Prote in and mRNA of Type Ⅰ、Ⅱ and Ⅲ Collagen in Human Osteosarcoma by Immunohistochemistry and in Situ Hybridization

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