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TIAN Xiao-yu, XING Hui, WONG Dan-hui, CHEN Gang, LU Yun-ping, MA Ding. The Construction of DNA-Pkcs Short-hairpin siRNA Recombinant Vector and Its Expression in Vitro[J]. Cancer Research on Prevention and Treatment, 2006, 33(09): 642-645，. DOI: 10.3971/j.issn.1000-8578.1325
Citation: TIAN Xiao-yu, XING Hui, WONG Dan-hui, CHEN Gang, LU Yun-ping, MA Ding. The Construction of DNA-Pkcs Short-hairpin siRNA Recombinant Vector and Its Expression in Vitro[J]. Cancer Research on Prevention and Treatment, 2006, 33(09): 642-645，. DOI: 10.3971/j.issn.1000-8578.1325
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The Construction of DNA-Pkcs Short-hairpin siRNA Recombinant Vector and Its Expression in Vitro

    Abstract
  • Objective To construct eukaryotic vector expressing shRNA(short hairpin RNA) of DNA-PKcs and study the significance of superexpression of DNA-PKcs in A2780 cells. Methods Designing two different shRNA targeting the coding sequence of DNA-PKcs, the pSIREN-DNA-PKcs shRNA was constructed by inserting the designed shRNA to the plasmid vector pSIREN.The human ovarian cancer cell strain A2780 was transfected by pSIREN-DNA-PKcs shRNA. The Expression of DNA-Pkcs in transfercted cells was detected by RT-PCR and West...
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