Proliferation and Identification of the Dendritic Cells from the Peripheral Blood of Patients with Cervical Cancer

Objective 　To culture and proliferate dendritic cells from the peripheral blood of patients with cervical cancer, to observe the morphologic, phenotypic characteristics of these cells and its potentiality to stimulate the proliferation of allo-T lymphocytes. Methods 　Monocytes were isolated f rom the peripheral blood of patient s and cultured with the cytokines ( GM-CSF, IL-4, TNF-а). The morphologic charac-teristics of those cells were observed with optics and elect ron microscopes and the phenotypic figures were analyzed with FACS. The proliferating activity of T lymphocytes was determined by MTT in vitro. Results 　Large amount of mature DCs could be obtained from the peripheral blood of patients with cervical cancer . DCs expressed high level of HLA-DR、CD1a 、CD80, and were potent to stimulate the proliferation of allo-T cells. Conclusion 　The mature dendritic cells could be obtained by culturing the peripheral blood of patients with cervical cancer, which set up the further foundation for clinical biotherapy of cervical cancer.