Enhanced sensitivity of human ovarian cancer cells to cisplatin by exogenous p53 gene

Objective To explore the effects of exogenous p53 gene on chemosensitivity of human ovarian cancer cell line SKOV-3. Methods human wild-type p53 gene eukaryotic expression vector was introduced into SKOV-3 cells by lipofectin-mediated gene transfection and the expression of p53 gene in them was de- tected by Northern blot and Western blot. The effects of cisplatin to the transfected cells was observed by colony formation and apoptosis. Results The exogenous p53 gene was e...