The contrast of telomerase activity between cytoplasmic and nuclear extracts of hepatocellular carcinoma samples

Objective To compare the telomerase activity in cytoplasmic and nuclear extracts of hepato- cellular carcinoma (HCC) samples. Methods Telonierase activitiy in cytoplasmic and nuclear extracts of HCC samples was analyzed by an unamplified telomerase assay based on the radioactive incorporation and liquid scintillation counting and silver-staining TRAP (telorneric repeat amplification protocol). Results Both of the cytoplasmic and nuclear extracts were found to express telo...