A Study on the Expression of Bmi-1 in SKM-1 Cell Line in vitro

Objective To determine the role of Bmi-1 gene and its coded protein in the incidence of myelodysplasia syndrome. Methods To determine the expression of Bmi-1on the level of mRNA and protein in MDS cell line SKM-1 by means of semi-quantities RT-PCR and Western Blot technique, normal bone marrow and cell line K562 as negative and positive comparison specifically. Results Bmi-1expression level SKM-1 differs from that of normal bone marrow remarkably, while the difference is not remarkable from that of K562. Co...