Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells

HUANG Xixiang; CAI Xiaoxuan; WANG Tianji; HUANG Miaoen; LI Li; LYU Yingnian

doi:10.3971/j.issn.1000-8578.2022.22.0172

Cancer Research on Prevention and Treatment > 2022 > 49(8): 780-785. > DOI: 10.3971/j.issn.1000-8578.2022.22.0172

HUANG Xixiang, CAI Xiaoxuan, WANG Tianji, HUANG Miaoen, LI Li, LYU Yingnian. Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2022, 49(8): 780-785. DOI: 10.3971/j.issn.1000-8578.2022.22.0172

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Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells

HUANG Xixiang^{1, 2},
CAI Xiaoxuan^{1, 3},
WANG Tianji^{1, 2},
HUANG Miaoen^{1, 3},
LI Li^{1, 2},
LYU Yingnian^{1, 2, 3, ,}

1.
School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, China
2.
Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang 524023, China
3.
Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524023, China

Funding:

Fund of Southern Marine Science and Engineering Guangdong Laboratory ZJW-2019-07

Science and Technology Program of Guangdong Province 2019B090905011

More Information

Corresponding author:
LU Wenping, E-mail: lyn7591@hotmail.com
Received Date: February 27, 2022
Revised Date: May 17, 2022
Available Online: January 12, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the photodynamic antitumor activity and chemical characteristics of pheophorbide A (PPBa)in vitro.
Methods
Breast cancer cells (MCF-7), lung cancer cells (A549), cervical cancer cells (HeLa), and three kinds of hepatoma cells (HepG2, hep3B and sk-Hep1) were planted in 96-well plates.The effects of light and dark toxicity, light intensity, and drug concentration on the phototoxicity of PPBa were investigated by MTT, and the uptake of PPBa was observed by Hoechst staining under a confocal microscope.The production of singlet oxygen was observed by flow cytometry and confocal microscopy with the reactive oxygen species detection kit.The photobleaching of PPBa was investigated by measuring the absorbance by a microplate reader according to the luminescence characteristics of PPBa.
Results
PPBa showed strong phototoxicity and low dark toxicity to six kinds of cancer cells, and IC₅₀ values to cancer cells were as follows: MCF-7:1.033 μmol/L, A549:1.911 μmol/L, HeLa: 2.319 μmol/L, HepG2:2.015 μmol/L, Hep3B: 2.089 μmol/L, sk-Hep1:2.467 μmol/L.The main uptake site of PPBa was the cytoplasm.The production of singlet oxygen was strongly dependent on the administration concentration, and photobleaching was very low.
Conclusion
For the six kinds of cancer cells, PPBa has the highest phototoxicity to breast cancer cells (MCF-7), with excellent properties and ideal photosensitizer characteristics.
- Pheophorbide A,
- Photodynamic therapy,
- Anti-tumor effect,
- Phototoxicity

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References (20)

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Phototoxicity of Pheophorbide A Extracted from Gracilaria Lemaneiformis on Six Kinds of Cancer Cells