Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells

HU Wenbing; WANG Gangsheng; CHEN Xi; CHEN Jun; YU Xifang

doi:10.3971/j.issn.1000-8578.2016.12.009

Cancer Research on Prevention and Treatment > 2016 > 43(12): 1055-1058. > DOI: 10.3971/j.issn.1000-8578.2016.12.009

HU Wenbing, WANG Gangsheng, CHEN Xi, CHEN Jun, YU Xifang. Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells[J]. Cancer Research on Prevention and Treatment, 2016, 43(12): 1055-1058. DOI: 10.3971/j.issn.1000-8578.2016.12.009

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Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells

Department of Oncology, Huangshi Central Hospital, Edong Healthcare, Huangshi 435000, China

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Corresponding author:
YU Xifang, E-mail:13972784990@139.com
Received Date: April 18, 2016
Revised Date: June 11, 2016
Available Online: February 04, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the effect of down-regulating the expression of KLF8 by siRNA interference on the invasion of human nasopharyngeal carcinoma cells CNE1-LMP1 and related mechanism.
Methods
The siRNA targeting KLF8 was designed and the eukaryotic expression plasmid was synthesized. The plasmid was transfected into CNE1-LMP1 cells. The expression of KLF8, E-cadherin and N-cadherin protein and mRNA were detected by Western blot and RT-PCR. Transwell invasion assay was employed to observe the invasion ability of CNE1-LMP1 cells through KLF8 siRNA.
Results
The expression levels of KLF8 protein and mRNA were down-regulated in the KLF8-siRNA-transfected CNE1-LMP1 cell lines compared with negative control (NC-si) group, which verified that the stable transfected cell lines were successfully established. KLF8 siRNA could up-regulate the expression of E-cadherin and down-regulate the expression of N-cadherin in CNE1-LMP1 cells. Transwell invasion assay showed that siRNA decreased the invasion ability of CNE1-LMP1 cells.
Conclusion
KLF8 is highly expressed in nasopharyngeal carcinoma cells CNE1-LMP1. KLF8 siRNA could affect the expression of E-cadherin and N-cadherin, and inhibit the invasion and metastasis of CNE1-LMP1 cells.
- KLF8,
- CNE1-LMP1 cells,
- RNA interference,
- EMT,
- Invasion

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References (24)

References

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Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells