To investigate the effect of sanguinarine on the invasion and migration of lung adenocarcinoma cells and its preliminary molecular mechanism.

MTT assay was used to observe the proliferative capacity of A549 and H1975 cells. Wound healing and Transwell assays were performed to determine the invasion and migration abilities of A549 and H1975 cells, respectively. RT-qPCR and Western blot were used to detect the mRNA and protein expression of core genes in Wnt/β-catenin signaling pathway.

Compared with the control group, sanguinarine(1-10μmol/L) could inhibit the activity of human lung adenocarcinoma cells in a concentration- and time- dependent manner (P < 0.05); the migration and invasion abilities of A549 and H1975 cells were significantly weakened by 1μmol/L sanguinarine after 48 h (P < 0.01); sanguinarine (1, 2.5, 5μmol/L) could significantly down-regulate the protein expression of β-catenin, phosphorylated GSK3β and DVL2 in Wnt/β-catenin pathway, thereby inhibiting the mRNA levels of TCF/LEF and CyclinD1, in a concentration-dependent manner (P < 0.05).

Sanguinarine may inhibit the migration and invasion of human lung adenocarcinoma cells by down-regulating the Wnt/β-catenin signaling pathway.

To investigate the expression and function of lncRNA XIST in MCF-7 breast cancer cells.

The expression of XIST in MCF 10A human normal breast epithelial cells and MCF-7 human breast cancer cells were detected by qRT-PCR. Transient transfection technique was used to overexpress XIST in MCF-7 cells. MTT assay and Transwell assay were used to detect the changes of proliferation, migration and invasion of MCF-7 cells after XIST overexpression in MCF-7 cells. Bioinformatics and qRT-PCR were used to predict and detect the changes of miRNA expression which have potential binding sites of XIST. qRT-PCR was used to detect the expression of XIST in MCF-7 cells which was transfected instantaneously by miR-130b-3p inhibitor.

XIST expression was decreased in MCF-7 cells, while miR-130b-3p expression was increased, compared with those in MCF-10A cells (both P < 0.001). Overexpression of XIST significantly inhibited the proliferation of MCF-7 cells (P < 0.001), promoted cell migration and invasion. And the expression level of miR-130b-3p in MCF-7 cells was reduced significantly after overexpression of XIST (P < 0.01), while the expression level of XIST in MCF-7 cells was increased significantly after down-expression of miR-130b-3p (P < 0.001).

The expression levels of XIST and miR-130b-3p in breast cancer MCF-7 cells are negatively correlated, and the overexpression of XIST could significantly inhibit the proliferation, and promote the migration and invasion of MCF-7 cells.

To investigate the effect of ATG5 on the senescence and proliferation of colon cancer HT29 cell line.

The senescence of colon cancer HT29 cell line was induced by doxorubicin. ATG5 expression was detected by Western blot. Low expression of ATG5 was detected by transient transfection of small interfering RNA, and the proportion of cell senescence and proliferation ability were detected by beta-galactosidase staining and CCK assay, respectively. At the same time, we added Rapa to induce autophagy and detected the proportion of cell senescence and proliferation ability. The effect of ATG5 on cell senescence related pathway proteins P53 and P21 was detected by Western blot.

The protein expression of ATG5 was down-regulated by doxorubicin-induced cell senescence. The mean number of senescent cells in the SiNC control group was 3±1, while that in the SiATG5 group was 12±2, and that in the low-expression ATG5 plus Rapa group was 12±1. Low expression of ATG5 could up-regulate the expression of senescence-related proteins P53 and P21.

Down-regulation of ATG5 promotes the senescence of colon cancer cells by up-regulating p53/p21.

To investigate the mechanism of miR-451 regulating the resistance of gastric cancer to 5-Fu by down-regulating the expression of MRP.

Real-time quantitative PCR was used to detect the relative expression of miR-451 in 20 pairs of human gastric cancer tissues and adjacent tissues and gastric cancer cell lines. The expression of miR-451 in the resistance group and non-resistant group were detected. We constructed gastric cancer cell line with stable plent-miR-451 overexpression, and the overexpression efficiency of miR-451 in gastric cancer cell line was detected by real-time quantitative PCR. The proliferation activity of the cells was detected by CCK8. The miR-451 target gene was analyzed by bioinformatics method and verified by luciferase assay. The effect of miR-451 on the mRNA and protein of targeted gene MRP were detected by real-time quantitative PCR and Western blot.

The expression of miR-451 in normal tissues was higher than that in cancer tissues, and miR-451 expression in the non-resistant gastric cancer tissues was higher than that in the drug-resistant gastric cancer tissues. The overexpression of miR-451 reduced the resistance of gastric cancer cells to 5-Fu treatment (P=0.0006). Bioinformatics analysis showed that MRP was the target gene of miR-451. Luciferase assay also confirmed that MRP was a potential target gene of miR-451. miR-451 overexpression decreased the mRNA and protein of the drug resistance gene MRP (P=0.00069).

Overexpression of miR451 could down-regulate MRP expression, which reduces the resistance of tumor cells to 5-Fu.

This study aimed to explore the status quo, hot topics, and future prospects in the field of tumor hypoxia by scientometric analysis.

The literatures about tumor hypoxia were downloaded from the Web of Science Core Collection from inceptions to Dec.31^st. 2018. We used CiteSpace 5.4.R1, VOSviewer 1.6.10 and Excel 2016 to analyze literature information, such as country, institution, author, keywords and references.

A total of 4399 papers about tumor hypoxia were identified, involving 20157 authors from 3604 institutions in 84 countries. Vaupel P, Harris AL and Dewhirst MW published the most literatures. United States, China and United Kingdom contributed the most publications. The three most contributed institutions are the University of Toronto, Stanford University and Oxford University. International Journal of Radiation Oncology Biology Physics (n=175, IF₂₀₁₇=5.554), Cancer Research (n=144, IF₂₀₁₇=9.13) and Radiotherapy Oncology (n=114, IF₂₀₁₇=4.942) are the most productive journals. The main hot topics in tumor hypoxia field are tumor hypoxic cells and cytokines, tumor hypoxia therapy, tumor hypoxia diagnosis, tumor hypoxia prognosis.

Developed countries in Europe and America are dominant in the field of tumor hypoxia research. The diagnosis and treatment of tumor hypoxic is still a difficult problem, especially how to overcome the drug resistance caused by tumor hypoxic.

To investigate the effect of Da Vinci robot radical gastrectomy on gastrointestinal motility and hormones of gastric cancer patients.

We selected 217 patients who underwent radical gastrectomy. According to different surgical methods, the patients were divided into Da Vinci operation group and open operation group.The recovery time of bowel sounds and the time of first anal exhaust after operation were recorded. The levels of gastrointestinal hormones, surgical-related factors and inflammatory factors were measured before and after operation.

The recovery time of bowel sounds and the first time of anal exhaust in the Da Vinci operation group were shorter than those in the open operation group(both P < 0.05). The motilin, gastrin, somatostatin levels at 24h after operation were significantly lower than those at 12h before operation in both groups(all P < 0.05), the vasoactive intestinal peptide levels were significantly increased(P < 0.05). At 24h after operation, the motilin and gastrin levels in the Da Vinci operation group were higher than those in the open operation group(both P < 0.05); the levels of vasoactive intestinal peptide in the Da Vinci operation group were lower than those in the open operation group(P < 0.05). In all patients, the recovery time of bowel sounds and the time of first anal exhaust were negatively correlated with the motilin and gastrin levels(P < 0.05), while they were positively correlated with the levels of vasoactive intestinal peptide at 24h after operation(all P < 0.05). There were significant differences in operative time, operative blood loss, drainage tube removal time, VAS pain grade and inflammatory factors between the two groups(all P < 0.05).

The recovery of gastrointestinal motility of gastric cancer patients treated with Da Vinci surgery is significantly faster than that with traditional laparotomy. The inflammation and stress reaction caused by trauma may be the important factors affecting gastrointestinal hormone secretion.

To compare the dosimetric characteristics of single-arc volumetric modulated arc therapy(VMAT) and 5-field dynamic intensity-modulated radiotherapy(dIMRT) for early-stage peripheral lung adenocarcinoma patients with spherical or spheroidal tumor lesions, and explore the appropriate curvature of single-arc VMAT.

We collected 32 early-stage peripheral lung adenocarcinoma patients with spherical or spheroidal tumor lesions and designed three treatment plans for selected cases: single-arc 180° and single-arc 360° VMAT and 5-field dIMRT. The volume of each parameter was compared.

The three types of plans could meet all the dosimetric requirements of the clinical target area. There was no significant statistical difference in maximum dose(D_max), minimum dose(D_min), mean dose(D_mean), homogeneity index(HI) and conformity index(CI) of PTV among three groups (P > 0.05). The mean lung dose (MLD), V₅, V₁₀ and V₂₀ of the bilateral-lungs and affected-lung in the dIMRT plan group were lower than those of the other two single-arc VMAT plans, and those of single-arc 360° VMAT plan were slightly higher than those of the single-arc 180° VMAT plan (P < 0.05). D_mean of spinal cord was the lowest in dIMRT group and the highest in single-arc 360° VMAT group (P < 0.05). D_mean of esophagus in dIMRT group was less than those in the single-arc VMAT groups (P < 0.05). MUs was the lowest in dIMRT group and the highest in single-arc 360° VMAT group (P < 0.05).

For early-stage peripheral lung adenocarcinoma patients with spherical or spheroidal tumor lesions, 5-field dIMRT is preferred; for single-arc VMAT, 180° single-arc is better.

To explore the relation between WNT7A methylation and clinical factors of NSCLC patients.

We selected 120 NSCLC patients as NSCLC group and 120 healthy people as control group. The detection rate of WNT7A methylation in two groups was compared by methylation specific polymerase chain reaction, and the relation between WNT7A methylation and clinical factors of NSCLC patients was compared.

The methylation rate of WNT7A in peripheral blood of NSCLC group was significantly higher than that in control group (P < 0.05). In peripheral blood of NSCLC group, there was no significant correlation between WNT7A gene methylation and gender, pathological type, age, smoking history(P > 0.05). The methylation rate of WNT7A gene was significantly correlated with tumor size, distant metastasis, TNM stage and pleural effusion(P < 0.05). The detection rate of WNT7A gene methylation in peripheral blood of NSCLC patients with pleural effusion, tumor size > 5cm and TNM stage Ⅲ-Ⅳ was significantly higher than that of patients without pleural effusion, tumor size ≤5cm and stage Ⅰ-Ⅱ (P < 0.05).

The detection rate of WNT7A gene methylation is high in NSCLC patients, which can be used as an effective diagnostic basis for NSCLC.

To investigate the relation between PKM2 and related pathological features of osteosarcoma, to further explore the markers for predicting the prognosis of osteosarcoma patients.

We collected 88 cases of paraffin-embedded primary osteosarcoma specimens, and the specimens were constructed by tissue microarray. Serial sections were used to detect the expression of PKM2 in the above tissues by immunohistochemistry. Kaplan-Meier analysis was used to demonstrate the prognostic value. Correlation analysis between parameters and patient's prognosis was performed using Cox regression model.

M2 macrophage-specific marker CD163 was elevated in osteosarcoma matrix, suggesting that M2 macrophages were densely infiltrated in osteosarcoma. PKM2 overexpression is positively correlated with M2 macrophage infiltration in osteosarcoma.

PKM2 overexpression and M2 macrophage infiltration were influence factors for patients' poor prognosis.

To analyze the characteristics of laboratory biomarkers associated with hypercoagulability in patients undergoing lung cancer surgery during the perioperative period and its diagnostic value for deep venous thrombosis (DVT).

We enrolled 143 patients treated with lung cancer surgery based on eligibility criteria. The lower extremity deep vein was examined perioperatively by compressed ultrasonic (CUS) to determine the DVT. The laboratory biomarkers associated with hypercoagulability were measured. We analyzed the area under curve (AUC) of ROC and cutoff in DVT (+) and DVT (-) groups with significant different biomarkers.

Thirty-three patients with DVT were first diagnosed from the postoperative 3rd day (POD3) to 7th day (POD7). The fibrinogen degradation product (FDP), D-Dimer, brain natriuretic peptide and C reactive protein were significantly increased in the postoperative POD1-POD7 (P < 0.05). There were significant differences in the change levels of FDP and D-D during the perioperative period between the DVT (+) and DVT (-) groups (P < 0.05). The AUC and diagnostic cutoff values of POD1-FDP, POD3-FDP, POD3-D-D were 0.726 and 4.65μg/ml, 0.811 and 4.85 μg/ml, 0.772 and 1.55 μg/ml, respectively. The sensitivity and specificity of serial test were 46.5% and 94.9% for the combined diagnosis.

The value of increased levels of POD1-FDP, POD3-FDP and POD3-D-Dimer for DVT diagnosis is moderate. The combined diagnosis with serial test could improve the specificity of diagnosis.

To retrospectively compare the efficacy between definitive external-beam radiotherapy (ExRT) and radical prostatectomy(RP) on patients with localized high-risk prostate cancer (PCa).

We retrospectively studied 150 patients with high-risk PCa(T2b-T4N0M0) who underwent definitive ExRT or RP. The inclusion criteria for high-risk PCa were PSA≥20 ng/ml or above cT3 or GS≥8. The primary end point was biochemical failure free survival(BFFS), and the secondary end point was distant metastasis free survival(DMFS), cancer-specific survival(CSS) and overall survival(OS).

A total of 88 patients underwent definitive ExRT and androgen deprivation therapy(ADT), and the remaining 62 patients underwent RP and pelvic lymphadenectomy(PLND). The median age and follow-up time were statistically significant between ExRT and RP groups(P < 0.05). The biochemical failure(BF) rate in ExRT group was significantly lower than that in RP group(P < 0.001), while BFFS was significantly increased(P < 0.001). The DMFS, CSS and OS were not significantly different between the two groups.

BF rate after definitive ExRT in patients with localized high-risk PCa are significantly lower than those undergoing RP, while BFFS is significantly increased.

To observe the therapeutic effect of elemene emulsion injection combined with chemoradiotherapy and its effect on immune function in nasopharyngeal carcinoma (NPC) patients.

Random number method was used to divide the patients into control group (IMRT+fluorouracil+cisplatin) and observation group (IMRT+fluorouracil+cisplatin+elemene injection 500 mg/d). We evaluated the short-term efficacy, the changes of T lymphocyte subsets in peripheral blood and adverse reactions.

All patients successfully completed the treatment. There was statistically significant difference between control group and observation group in the total effective rate (71.74% vs. 82.61%, P < 0.05), CD8⁺ level in peripheral blood lymphocyte subsets (31.35±2.39 vs. 34.37±1.97, P < 0.05), the ratio of CD4⁺/CD8⁺ (1.37±0.03 vs. 0.98±0.18, P < 0.05), and the quality of life (57.2±7.4 vs. 60.3±5.3, P < 0.05). Compared with control group, the observation group had lower hematological toxicity and grade Ⅲ-Ⅳ skin reactions, and the incidence of grade Ⅲ-Ⅳ gastrointestinal reactions (nausea, vomiting) was significantly lower (P < 0.05).

Elemene combined with chemoradiotherapy could improve the quality of life of nasopharyngeal carcinoma patients, reduce the incidence of adverse reactions and obtain a better prognosis.

To conduct a meta-analysis on the data of S-1 and capecitabine or fluorouracil in the treatment of advanced colorectal cancer, to demonstrate the feasibility of S-1 replacing capecitabine or fluorouracil.

We searched published literature in the PubMed, Cochrane Library, Embase and CNKI databases, and searched for controlled trials at the Clinical Trials Center (https://www.clinicaltrials.gov/). The data extraction and quality evaluation were conducted according to Cochrane Handbook 5.1.0, and RevMan 5.3 software was used for the meta-analysis of relevant case-control studies.

The data of 3462 patients from 11 randomized controlled trials were included. Compared with the capecitabine/fluorouracil regimen, OS was improved in the S-1 treatment group, but there was no statistically significant difference in PFS, DCR or ORR. The incidence of diarrhea, renal insufficiency and hand-foot syndrome in the S-1 group was lower than that in the control group. But the incidence of platelet reduction in the S-1 group was higher than that in the control group at a reasonable contrast dose.

S-1 has a certain therapeutic advantage after replacing capecitabine or fluorouracil in conventional chemotherapy on advanced colorectal cancer.

To evaluate adverse reactions of the hyperthermic intraperitoneal chemotherapy (HIPEC) combined with intravenous chemotherapy in the treatment of ovarian cancer(OC) after the cytoreductive surgery (CRS), and to analyse the effect of adverse reactions on the survival time of patients.

We selected 163 patients with ovarian cancer. All patients got CRS for OC, and then were divided into two groups according to the different treatment modes: observation group (n=47, HIPEC in combination of intravenous chemotherapy) and control group (n=116, intravenous chemotherapy). The adverse reactions of the two groups were observed. All patients were followed up after operation.

The incidence of hypoproteinemia in the observation group was higher than that in the control group, while the incidence of leukopenia, thrombocytopenia, abdominal distention and abdominal pain were lower than those in the control group (P < 0.05), and the other adverse reactions were not statistically different. The incidence of D-Dimer increase, hypocalcemia, hyperglycemia as well as nausea and vomiting in patients with > 2 cycles of HIPEC were increased (P < 0.05). There was no significant difference in overall survival rate between the two groups. The survival of patients without anemia or mild anemia in the observation group was better than that of patients with severe anemia (P < 0.05). The occurrence of other adverse reactions had no significant effect on the overall survival rate of the patients.

HIPEC combined with intravenous chemotherapy after CRS would not increase adverse reactions, especially severe adverse reactions in OC patients, but there is a high incidence of hypoproteinemia.