To explore the influences of SLC39A5 knockout on the establishment of esophageal cancer model induced by 4-nitroquinoline 1-oxide (4-NQO) in C57BL/6 mice.

Ten wild-type mice were treated as negative control group and drank the 1, 2-propylene glycol at 100 μg/ml; 140 wild-type mice and 80 knockout genotype mice were treated as experimental groups and drank the carcinogen 4-NQO stock solution dissolved in 1, 2-propylene glycol at 100μg/ml, the experimental time was 28 weeks and all three groups' mice were sacrificed.

The survival rates were 100%, 92.96% and 91.25% in negative group, wild-type experimental group and SLC39A5 knockout genotype experimental group, respectively; the rates of tumor formation were 0, 61.36% and 28.77%, and there was a statistical difference between the two experimental groups(χ²=19.98, P < 0.001).

The esophageal cancer model in C57BL/6 mice and SLC39A5 knockout mice are established successfully and the facilitated role of SLC39A5 in the occurrence and development of esophageal cancer is verified.

To detect the expression and the function of microRNA-155(miR-155) in hepatocellular carcinoma(HCC) tissuse, and to elucidate related mechanism.

miR-155 expression was examined in 30 pairs of HCC and adjacent normal tissues by quantitative reverse transcription PCR(qRT-PCR). HCC cells were treated with miR-155 mimic to upregulate miR-155 expression in vitro. CCK-8 and Transwell assays were used to analyze the changes in cell proliferation and migration. PTEN was predicted to be the direct target of miR-155 by bioinformatics analysis and luciferase reporter assay. The prognostic value of miR-155 and PTEN in patients with HCC were assessed through cBioPortal database.

miR-155 was significantly upregulated in HCC tissues compared with that in adjacent normal tissues (P < 0.0001). Overexpression of miR-155 was associated with worse disease free survival (P=0.021). Elevated expression of miR-155 promoted the proliferation and migration of hepatocellular carcinoma cells. Furthermore, miR-155 directly repressed PTEN expression, which enhanced PI3K signaling pathway. Deletion of PTEN depicted worse disease free survival in patients with HCC (P=0.0133).

The axis of miR-155/PTEN accelerates HCC progression.

To explore PDK4 mRNA expression in breast cancer tissues and its relationship with prognosis.

We analyzed the mRNA expression of PDK4 by the Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) and estimated the correlation of PDK4 mRNA expression with the prognosis of breast cancer patients by Kaplan-Meier plotter (www.kmplot.com). Real-time PCR was used to detect the mRNA expression of PDK4 in TGF-β-treated MCF-7 cell line. siRNA decreased the mRNA level of PDK4 in breast cancer cells. Transwell assay was used to detect the invasion ability of MCF-7 cells.

PDK4 was down-regulated in breast cancer tissues, and the low expression of PDK4 was positively correlated with poor prognosis of breast cancer patients. In breast cancer cell lines, TGF-β decreased PDK4 mRNA expression, and the low expression of PDK4 enhanced the invasion of MCF-7 cells.

The low mRNA level of PDK4 is an important prognosis indicator for breast cancer.

To explore a new cut-off of remnant liver volume-to-body weight ratio (RLV-BWR) and investigate the relationship between RLV-BWR and post-hepatectomy liver failure (PHLF) in patients with hepatocellular carcinoma (HCC) of cirrhotic liver.

We analyzed the clinical data of 181 patients who underwent hemihepatectomy. Total liver volume, tumor volume, remnant liver volume and resected liver volume were measured by Myrian-Liver surgical planning system before surgery. Intraoperative resected liver volume(include resected normal liver and tumor volume) were collected by drainage method. Patients were divided into PHLF group and non-PHLF group according to the "50-50"criteria. Then the risk factors of PHLF were analyzed. The cut-off of RLV-BWR and efficiency to predict PHLF were analyzed in the subgroup of cirrhotic liver. The grading of liver cirrhosis was analysed by helical CT, retrospectively.

After operation, 22 patients developed PHLF and one patient died of PHLF. Logistic regression analysis showed that preoperative total bilirubin level and RLV-BWR were identified as independent predictors of PHLF. According the postoperative pathological data, 102 patients with cirrhotic liver were selected; 18 patients developed PHLF and one patient died of PHLF in the subgroup. ROC curve analysis showed that the cut-off of RLV-BWR was 0.94%(the areas under the curve was 0.853, P < 0.01, sensitivity and specialty rates were 94.4% and 72.3%) in patients with HCC in cirrhotic liver. By analyzing helical CT, 84 cases were grade Ⅰ or Ⅱ and 18 cases were grade Ⅲ.

The risk of PHLF would increase in the HCC in cirrhotic liver patients with RLV-BWR ≤0.94% after hemihepatectomy.

To observe the states of hepatitis B virus infection and the expression levels of HBsAg and HBsAb in patients with colon cancer, and to analyze their correlation with the staging and prognosis of colon cancer.

We collected 85 cases of colon cancer treated in Fujian Provincial Hospital. SPSS 16.0 software was used to retrospectively study the possible influence of hepatitis B virus infection on the infiltration depth, regional lymph node metastasis and distant metastasis in patients with colon cancer.

There was no statistically significant difference between the states of hepatitis B virus infection and the depth (T), the regional lymph node metastasis (N) of colon cancer (P=0.331, P=0.098). However, the metastatic disease of regional lymph node in subgroup of hepatitis-B-positive colon cancer was significantly lower than that in hepatitis-B-negative colon cancer(8.1% vs. 17.8%). The level of serum HbsAb in patients without regional lymph node metastasis was higher than that with regional lymph node metastasis (240.111 mIU/ml vs. 178.161 mIU/ml). There was statistically significant difference between the states of hepatitis B virus infection and the distant metastasis (M), liver metastasis (P < 0.001). Meanwhile, both subgroups of positive hepatitis B with infection and vaccine showed significant statistical differences when comparing with hepatitis B negative in the event of the probability of liver metastases (P=0.039 and P < 0.001).

The high expression of serum HBsAb might have a potential inhibitory effect on region lymph node metastases in patients with colon cancer. The state of positive hepatitis B expression shows a significantly negative correlation with distant metastases, especially liver metastases. The states of hepatitis B virus infection and the levels with serum HbsAg/HbsAb influence the staging and prognosis of patients with colon cancer significantly.

To analyze the cardiac safety of trastuzumab on HER2 positive breast cancer patients.

Trastuzumab was administrated every three weeks in 185 patients with HER2 positive breast cancer. The initial dose was 8 mg/kg, and the subsequent dose was 6 mg/kg, for 4-36 cycles. The cardiotoxicity of these patients was analyzed.

The median treatment was 17 cycles in Her-2 positive breast cancer patients who received trastuzumab. Left ventricular ejection fraction (LVEF) level dropped in 88 cases (47.6%) after trastuzumab treatment; 74 patients (40.0%) occurred valvular regurgitation (new+ aggravation); 16 patients (8.0%) occurred the reduction of diastolic function of left ventricle; 88 patients (47.6%) experienced cardiotoxicity, among which 83 patients experienced gradeⅠcardiotoxicity and five patients experienced grade Ⅱ cardiotoxicity, no patient experienced grade Ⅲ cardiotoxicity.

The treatment of trastuzumab on Chinese patients with HER2 positive breast cancer shows a satisfactory cardiac safety. However, the potential cardiotoxicity of trastuzumab should be carefully monitored during therapy.

To compare the minimally invasive effect of Da Vinci robotic surgery with video-assisted thoracoscopic surgery(VATS) in the lobectomy.

We analyzed 35 patients that underwent Da Vinci robotic surgery and 66 patients that underwent VATS. We collected blood loss, operative time, volume and duration of drainage, the number of resected lymph nodes and lymph node stations and postoperation hospitalization time. And postoperative complications were also included.

Blood loss, operative time, number of resected lymph node stations, number of resected lymph nodes, volume of drainage, time of drainage, postoperative hospitalization time and postoperative complications(hoarseness and chylothorax) showed no significant statistical difference between two surgeries(P > 0.05).

Da Vinci robotic surgery is safe and feasible. Although the robotic surgery is slightly superior to VATS in operative time, blood loss, volume and duration of drainage, post-operation hospitalization time and postoperative complications, there is no statistically significant difference.

To analyse breast cancer incidence and mortality based on the data of Hubei cancer registries in 2012.

According to the breast cancer data collected from seven Hubei cancer registries, descriptive analysis included Hubei breast cancer incidence, distribution and mortality in 2012 stratified by areas, gender, age and cancer site.

A total of 1830 cases of newly diagnosed breast cancers were reported in seven Hubei registries, including 1809(98.85%) female patients, accounting for 16.75% of female cancers; 443 cases breast cancer deaths, including 433(97.74%) cases of female breast cancer deaths, accounting for 8.31% of female cancer deaths. The crude incidence of female breast cancer was 44.81 per 10 million, among which, urban areas was 62.53 per 10 million and rural areas was 27.46 per 10 million; mortality was 10.73 per 10 million, among which, urban areas was 14.57 per 10 million and rural areas was 6.96 per million. Breast cancer incidence was the highest among female cancers, and the breast cancer mortality ranked as the fifth among female cancers. Female breast cancer incidence was bimodal distribution of 60-64 and 75-79 year-old groups; mortality increased within 0-59 years old and decreased slowly after 59 years old, then rapidly rose after 74 years old.

There is a significant difference of the breast cancer incidence and mortality between the annual Hubei Province's data and annual national data, providing a reliable basis according to which the government and the relational department could formulate prevention and control strategies and planning.