2012 Vol. 39 No. 07
2012, 39(07): 765-768.
DOI: 10.3971/j.issn.1000-8578.2012.07.001
Abstract:
Objective To explore the effects of Cisplatin(DDP) and 5-fluorouracil(5-Fu) on the expression of NKG2D ligands of human esophagus carcinoma cell EC9706 and cytokine-induced killer (CIK) cells cytotoxicity. Methods The IC50 of DDP,5-Fu against EC9706 cells were measured by MTT assay.Expressions of signal pathway molecules involved in DNA damage and repair system were detected by RT-PCR.The expression of NKG2D ligands (MICA,MICB,ULBP1,ULBP2,ULBP3) were analyzed by flow cytometery.Cytotoxicities of CIK cells against EC9706 cells before and after cultured by 1/2 IC50 DDP or 5-Fu were analyzed by LDH releasing assay at effector-to-target cell ratio(E∶T) of 20∶1. Results DDP,5-Fu could decrease the proliferation and survival rate of EC9706 cells,the IC50 was 5 μg/ml and 10 μg/ml,respectively,which increased mRNA expressions of signal pathway molecules involved in DNA damage and repair system.MICA,MICB,ULBP2,ULBP3 on EC9706 cells were over expressed after 72 h cultured with 1/2 IC50 DDP,while expression of MICA,ULBP2,ULBP3 were higher after treated by 1/2 IC50 5-Fu.Cytotoxicity of CIK cells against EC9706 cells cultured by 1/2 IC50 DDP,1/2 IC50 5-Fu were(37.08±0.62)%,(59.33±2.10)%,(52.44±0.97)%,respectively.Cytotoxicity of CIK cells against EC9706 cells cultured by either 1/2 IC50 DDP or 1/2 IC50 5-Fu was significantly enhanced. Conclusion The results indicate that DDP or 5-Fu can enhance the susceptibility of EC9706 cells to CIK cells-mediated killing effect by upregulating the expressions of NKG2D ligands through activating signal pathway molecules involved in DNA damage and repair system.
Objective To explore the effects of Cisplatin(DDP) and 5-fluorouracil(5-Fu) on the expression of NKG2D ligands of human esophagus carcinoma cell EC9706 and cytokine-induced killer (CIK) cells cytotoxicity. Methods The IC50 of DDP,5-Fu against EC9706 cells were measured by MTT assay.Expressions of signal pathway molecules involved in DNA damage and repair system were detected by RT-PCR.The expression of NKG2D ligands (MICA,MICB,ULBP1,ULBP2,ULBP3) were analyzed by flow cytometery.Cytotoxicities of CIK cells against EC9706 cells before and after cultured by 1/2 IC50 DDP or 5-Fu were analyzed by LDH releasing assay at effector-to-target cell ratio(E∶T) of 20∶1. Results DDP,5-Fu could decrease the proliferation and survival rate of EC9706 cells,the IC50 was 5 μg/ml and 10 μg/ml,respectively,which increased mRNA expressions of signal pathway molecules involved in DNA damage and repair system.MICA,MICB,ULBP2,ULBP3 on EC9706 cells were over expressed after 72 h cultured with 1/2 IC50 DDP,while expression of MICA,ULBP2,ULBP3 were higher after treated by 1/2 IC50 5-Fu.Cytotoxicity of CIK cells against EC9706 cells cultured by 1/2 IC50 DDP,1/2 IC50 5-Fu were(37.08±0.62)%,(59.33±2.10)%,(52.44±0.97)%,respectively.Cytotoxicity of CIK cells against EC9706 cells cultured by either 1/2 IC50 DDP or 1/2 IC50 5-Fu was significantly enhanced. Conclusion The results indicate that DDP or 5-Fu can enhance the susceptibility of EC9706 cells to CIK cells-mediated killing effect by upregulating the expressions of NKG2D ligands through activating signal pathway molecules involved in DNA damage and repair system.
2012, 39(07): 769-772.
DOI: 10.3971/j.issn.1000-8578.2012.07.002
Abstract:
Objective To observe metastatic effect of Hui medicine Aikangfang in C57 mice Lewis lung cancer cells and test bcl-2 expression in each mice tumor tissue. Methods Fifty-five tumor-bearing mice were randomly divided into model group,chemotherapy(CTX) group,low-dose group,low-dose with chemotherapy group and high-dose with chemotherapy group.After treatment,observing the lung surfaces metastasis and bcl-2 protein expressions of each mouse. Results (1) Lung metastases changes:compared with tumor-bearing model,the more than mice lung metastases is statistically significant difference (P<0.05).(2) bcl-2 expression:compared with tumor-bearing model,the more than bcl-2 expression is statistically significant difference (P<0.01). Conclusion Hui medicine Aikangfang can resist lung cancer metastasis in mice and reduce bcl-2 expression.
Objective To observe metastatic effect of Hui medicine Aikangfang in C57 mice Lewis lung cancer cells and test bcl-2 expression in each mice tumor tissue. Methods Fifty-five tumor-bearing mice were randomly divided into model group,chemotherapy(CTX) group,low-dose group,low-dose with chemotherapy group and high-dose with chemotherapy group.After treatment,observing the lung surfaces metastasis and bcl-2 protein expressions of each mouse. Results (1) Lung metastases changes:compared with tumor-bearing model,the more than mice lung metastases is statistically significant difference (P<0.05).(2) bcl-2 expression:compared with tumor-bearing model,the more than bcl-2 expression is statistically significant difference (P<0.01). Conclusion Hui medicine Aikangfang can resist lung cancer metastasis in mice and reduce bcl-2 expression.
2012, 39(07): 773-775.
DOI: 10.3971/j.issn.1000-8578.2012.07.003
Abstract:
Objective To detect the effect of phytoestrogen on DMBA induced breast cancer development of young female SD rats. Methods The 30 rats were randomly divided into two groups:experimental group and control group.All rats were given DMBA,the experimental group was additionally fed with soy isoflavones,and the incidence of breast cancer,the tumor diameter,the results of immunohistochemistry were compared between the two groups. Results For the young SD rats,phytoestrogens can significantly reduce the DMBA-induced breast cancer incidence,tumor size,and immunohistochemical results(P<0.05). Conclusion Phytoestrogens can reduce young female SD rats incidence of breast cancer induced by DMBA.Early childhood interventions can reduce the incidence of breast cancer and improve the prognosis.
Objective To detect the effect of phytoestrogen on DMBA induced breast cancer development of young female SD rats. Methods The 30 rats were randomly divided into two groups:experimental group and control group.All rats were given DMBA,the experimental group was additionally fed with soy isoflavones,and the incidence of breast cancer,the tumor diameter,the results of immunohistochemistry were compared between the two groups. Results For the young SD rats,phytoestrogens can significantly reduce the DMBA-induced breast cancer incidence,tumor size,and immunohistochemical results(P<0.05). Conclusion Phytoestrogens can reduce young female SD rats incidence of breast cancer induced by DMBA.Early childhood interventions can reduce the incidence of breast cancer and improve the prognosis.
2012, 39(07): 776-779.
DOI: 10.3971/j.issn.1000-8578.2012.07.004
Abstract:
Objective To explore the effects and molecular mechanisms of ethyl pyruvate (EP) on invasion and metastasis of human gastric cancer SGC-7901 cells. Methods MTT assay was used to evaluate IC50 of EP on human gastric cancer SGC-7901 cell line.After different concentrations of EP (0,10 mmol/L,20 mmol/L and 40mmol/L) were added into SGC-7901 cells,the expression levels of protein kinase B (Akt) and phosphorylated Akt (p-Akt) mRNA were identified by real-time PCR,and the expression levels of Akt,p-Akt,matrix metalloproteinase-2 (MMP-2) and MMP-9 protein were detected by Western blot.The invasive and metastatic activities of gastric cancer cells were evaluated by wound -healing and Transwell assay.SGC-7901 xenograft tumor model was established,and the protein expression of Akt,p -Akt,MMP-2 and MMP-9 was further validated by immunohistochemical analysis. Results The IC50 of EP on human gastric cancer SGC-7901 cells was about 36.73mmol/L.EP administration inhibited Akt mRNA expression,and downregulated Akt,p-Akt,MMP-2 and MMP-9 protein expression.Wound-healing assay indicated that the migration and exercise capability of SGC-7901 cells was reduced obviously;Transwell assay showed,in comparison with the control group (93.33±4.16),the number of invasive and metastatic cells infiltrated the matrigel in EP 10 mmol/L group(75.34±4.73),EP 20 mmol/L group (61.34±3.06) and EP 40 mmol/L group (39.00±3.00) was respectively decreased (each P<0.001).Immunohistochemical analysis further confirmed the expression levels of Akt,p-Akt,MMP-2 and MMP-9 protein shown by Western blot. Conclusion EP administration can inhibit the invasion and metastasis of human gastric cancer SGC-7901 cells via downregulation of Akt pathway,and it may play a crucial role in cancer therapy.
Objective To explore the effects and molecular mechanisms of ethyl pyruvate (EP) on invasion and metastasis of human gastric cancer SGC-7901 cells. Methods MTT assay was used to evaluate IC50 of EP on human gastric cancer SGC-7901 cell line.After different concentrations of EP (0,10 mmol/L,20 mmol/L and 40mmol/L) were added into SGC-7901 cells,the expression levels of protein kinase B (Akt) and phosphorylated Akt (p-Akt) mRNA were identified by real-time PCR,and the expression levels of Akt,p-Akt,matrix metalloproteinase-2 (MMP-2) and MMP-9 protein were detected by Western blot.The invasive and metastatic activities of gastric cancer cells were evaluated by wound -healing and Transwell assay.SGC-7901 xenograft tumor model was established,and the protein expression of Akt,p -Akt,MMP-2 and MMP-9 was further validated by immunohistochemical analysis. Results The IC50 of EP on human gastric cancer SGC-7901 cells was about 36.73mmol/L.EP administration inhibited Akt mRNA expression,and downregulated Akt,p-Akt,MMP-2 and MMP-9 protein expression.Wound-healing assay indicated that the migration and exercise capability of SGC-7901 cells was reduced obviously;Transwell assay showed,in comparison with the control group (93.33±4.16),the number of invasive and metastatic cells infiltrated the matrigel in EP 10 mmol/L group(75.34±4.73),EP 20 mmol/L group (61.34±3.06) and EP 40 mmol/L group (39.00±3.00) was respectively decreased (each P<0.001).Immunohistochemical analysis further confirmed the expression levels of Akt,p-Akt,MMP-2 and MMP-9 protein shown by Western blot. Conclusion EP administration can inhibit the invasion and metastasis of human gastric cancer SGC-7901 cells via downregulation of Akt pathway,and it may play a crucial role in cancer therapy.
2012, 39(07): 780-783.
DOI: 10.3971/j.issn.1000-8578.2012.07.005
Abstract:
Objective To investigate the sensitiziation of xiaoaiping injection to oxaliplatin in inhibitory of human ovarian cancer Caov-3 cells proliferation and explore the mechanism. Methods Caov-3 cells were treated with oxaliplatin combined with or without xiaoaiping,and the control group was treated with PBS.The inhibitory effects on cell proliferation were examined by MTT assay and oxaliplatin's IC50 was generated.The apoptosis morphological changes in Caov-3 cells were observed by fluorescent microscopy.Apoptosis peak was analyzed with FACScan flow cytometry.The expression of caspase-3 in Caov-3 cells was analyzed by Western blot. Results In Caov-3 cells,the value of IC50 of OXA combined with xiaoaiping were significantly lower than that of OXA alone.And cell survival rate of the group of OXA combined with xiaoaiping was lower than that of group treated by OXA alone,too.Besides,xiaoaiping increased OXA induced apoptosis in morphological and DNA contents' changes.In addition,the cleavage of caspase-3,a hallmark of apoptosis protein was increased in Caov-3 cells treated with oxaliplatin combined with xiaoaiping. Conclusion Xiaoaiping can sensitize the OXA in inhibition of human ovarian cancer Caov-3 cells proliferation.Activation of caspase-3 might be involved in the mechanisms of the sensitization.
Objective To investigate the sensitiziation of xiaoaiping injection to oxaliplatin in inhibitory of human ovarian cancer Caov-3 cells proliferation and explore the mechanism. Methods Caov-3 cells were treated with oxaliplatin combined with or without xiaoaiping,and the control group was treated with PBS.The inhibitory effects on cell proliferation were examined by MTT assay and oxaliplatin's IC50 was generated.The apoptosis morphological changes in Caov-3 cells were observed by fluorescent microscopy.Apoptosis peak was analyzed with FACScan flow cytometry.The expression of caspase-3 in Caov-3 cells was analyzed by Western blot. Results In Caov-3 cells,the value of IC50 of OXA combined with xiaoaiping were significantly lower than that of OXA alone.And cell survival rate of the group of OXA combined with xiaoaiping was lower than that of group treated by OXA alone,too.Besides,xiaoaiping increased OXA induced apoptosis in morphological and DNA contents' changes.In addition,the cleavage of caspase-3,a hallmark of apoptosis protein was increased in Caov-3 cells treated with oxaliplatin combined with xiaoaiping. Conclusion Xiaoaiping can sensitize the OXA in inhibition of human ovarian cancer Caov-3 cells proliferation.Activation of caspase-3 might be involved in the mechanisms of the sensitization.
2012, 39(07): 784-786.
DOI: 10.3971/j.issn.1000-8578.2012.07.006
Abstract:
Objective To investigate the inhibition of G31P on the angiogenesis of the prostate cancer PC-3 cell in vivo. Methods The effect of G31P on angiogenesis of human prostate tumor of nude mice were observed in nude mice by building a human androgen-independent prostate cancer PC-3 (GFP-labeled) orthotopic transplantation tumor cells model. Results The tumor angiogenesis of G31P treated group (1.26±0.46)was significantly reduced (0.49±0.12,P<0.05) compared with the control group.VEGF(P<0.01)and NF-κB(P<0.01)expression of G31P treated groupwas significantly reduced (immunohistochemistry) compared with the control group. Conclusion G31P could inhibit the angiogenesis of the prostate cancer PC-3 cell in vivo.
Objective To investigate the inhibition of G31P on the angiogenesis of the prostate cancer PC-3 cell in vivo. Methods The effect of G31P on angiogenesis of human prostate tumor of nude mice were observed in nude mice by building a human androgen-independent prostate cancer PC-3 (GFP-labeled) orthotopic transplantation tumor cells model. Results The tumor angiogenesis of G31P treated group (1.26±0.46)was significantly reduced (0.49±0.12,P<0.05) compared with the control group.VEGF(P<0.01)and NF-κB(P<0.01)expression of G31P treated groupwas significantly reduced (immunohistochemistry) compared with the control group. Conclusion G31P could inhibit the angiogenesis of the prostate cancer PC-3 cell in vivo.
2012, 39(07): 787-792.
DOI: 10.3971/j.issn.1000-8578.2012.07.007
Abstract:
Objective To investigate the change in blood-brain barrier (BBB)permeability and ultrastructure of microvessle in C6 glioma and the tight junction protein expression of claudin-5,which were whole-brain irradiated with 15 Gy,and to explore the time-domain of BBB opened. Methods Sixty tumor-bearing rats (17th day after inoculation) were whole-brain irradiated with 15 Gy,and 10 rats were severally sacrificed at 24 h,3 d,7 d,14 d,21 d and 28 d after irradiation,5 rats were using transmission electron microscopy of exogenous lanthanum nitrate(La3+)-tracing to observe the BBB permeability of the tissues within 2 mm to the border of the tumor and using immunohistochemical method to detect the expression of claudin-5,respectively. Results (1)At 24 h,3 d after exposure,La3+ obviously exuded to the basement membrane(BM),extra-vascular,the space inside the brain tissue and cells,a little remaining in the endovascular.At 7 d after exposure,La3+ obviously exuded to the basement membrane and brain tissue space less than that in the previous days.At 14d after exposure,La3+ exuded to the local basement membrane.At 21 d,28 d after exposure,La3+ exudation was disappeared.Statistical analysis showed that 24 h,3 d>7 d>14 d>21 d,28 d (P<0.05).(2)claudin-5 expression was mild at 24 h and 3 d after irradiation,showed debris-like expression.claudin-5 expression was light and moderate at 7 d after exposure,combined with deformation and continuous expression.It was moderate expression 14 d after exposure,showed vascular distortion and deformation,but was still continuous state.It was medium-intensity expression at 21 d and 28 d after irradiation,appeared to express both a row and debris.Statistical analysis showed that 24 h,3 d<7 d<14 d<21 d,28 d(P<0.05). Conclusion BBB is opened and gradually resuming 2 weeks after irradiated with 15Gy in C6 glioma which is in time-dependent manner.
Objective To investigate the change in blood-brain barrier (BBB)permeability and ultrastructure of microvessle in C6 glioma and the tight junction protein expression of claudin-5,which were whole-brain irradiated with 15 Gy,and to explore the time-domain of BBB opened. Methods Sixty tumor-bearing rats (17th day after inoculation) were whole-brain irradiated with 15 Gy,and 10 rats were severally sacrificed at 24 h,3 d,7 d,14 d,21 d and 28 d after irradiation,5 rats were using transmission electron microscopy of exogenous lanthanum nitrate(La3+)-tracing to observe the BBB permeability of the tissues within 2 mm to the border of the tumor and using immunohistochemical method to detect the expression of claudin-5,respectively. Results (1)At 24 h,3 d after exposure,La3+ obviously exuded to the basement membrane(BM),extra-vascular,the space inside the brain tissue and cells,a little remaining in the endovascular.At 7 d after exposure,La3+ obviously exuded to the basement membrane and brain tissue space less than that in the previous days.At 14d after exposure,La3+ exuded to the local basement membrane.At 21 d,28 d after exposure,La3+ exudation was disappeared.Statistical analysis showed that 24 h,3 d>7 d>14 d>21 d,28 d (P<0.05).(2)claudin-5 expression was mild at 24 h and 3 d after irradiation,showed debris-like expression.claudin-5 expression was light and moderate at 7 d after exposure,combined with deformation and continuous expression.It was moderate expression 14 d after exposure,showed vascular distortion and deformation,but was still continuous state.It was medium-intensity expression at 21 d and 28 d after irradiation,appeared to express both a row and debris.Statistical analysis showed that 24 h,3 d<7 d<14 d<21 d,28 d(P<0.05). Conclusion BBB is opened and gradually resuming 2 weeks after irradiated with 15Gy in C6 glioma which is in time-dependent manner.
2012, 39(07): 793-797.
DOI: 10.3971/j.issn.1000-8578.2012.07.008
Abstract:
Objective To construct recombinant adenovirus expressing both simulated peptide of blood group B antigen P1/Fas and CCL19 by pAdEasy-1 packaging system,and test their expression. Methods We constructed pShuttle-CMV-P1/Fas-CCL19 by adenovirus Shuttle plasmid pShuttle-CMV and pAdEasy-1.Then,we transfected pAd-CMV-P1/Fas-CCL19 to HEK293A cells and obtained recombinant Ad-P1/Fas-CCL19 particles which can infect 4T1 cells.At last we detected mRNA and protein expression of P1/Fas-CCL19 in 4T1 cells.4T1 tumor-bearing mice model immuned by the B red blood cell was established successfully,and injected with recombinant adenovirus Ad-P1/Fas-CCL19,empty adenovirus,saline into the tumors,respectively.Then,tumor changes and survival period of the three groups were compared. Results P1/Fas-CCL19 can be effectively expressed in 4T1 cells on both mRNA and protein levels.Intratumor injection of recombinant adenovirus Ad-P1/Fas-CCL19 group inhibited 4T1 tumor growth significantly compared with intratumoral injection of adenovirus,empty saline,but survival time has no obvious difference among the three groups. Conclusion P1/Fas-CCL19 recombinant adenovirus vector was successfully constructed,experiments proved its stable expression and efficacy in vivo,in vitro,which make a foundation for further clinical research.
Objective To construct recombinant adenovirus expressing both simulated peptide of blood group B antigen P1/Fas and CCL19 by pAdEasy-1 packaging system,and test their expression. Methods We constructed pShuttle-CMV-P1/Fas-CCL19 by adenovirus Shuttle plasmid pShuttle-CMV and pAdEasy-1.Then,we transfected pAd-CMV-P1/Fas-CCL19 to HEK293A cells and obtained recombinant Ad-P1/Fas-CCL19 particles which can infect 4T1 cells.At last we detected mRNA and protein expression of P1/Fas-CCL19 in 4T1 cells.4T1 tumor-bearing mice model immuned by the B red blood cell was established successfully,and injected with recombinant adenovirus Ad-P1/Fas-CCL19,empty adenovirus,saline into the tumors,respectively.Then,tumor changes and survival period of the three groups were compared. Results P1/Fas-CCL19 can be effectively expressed in 4T1 cells on both mRNA and protein levels.Intratumor injection of recombinant adenovirus Ad-P1/Fas-CCL19 group inhibited 4T1 tumor growth significantly compared with intratumoral injection of adenovirus,empty saline,but survival time has no obvious difference among the three groups. Conclusion P1/Fas-CCL19 recombinant adenovirus vector was successfully constructed,experiments proved its stable expression and efficacy in vivo,in vitro,which make a foundation for further clinical research.
2012, 39(07): 798-801.
DOI: 10.3971/j.issn.1000-8578.2012.07.009
Abstract:
Objective To investigate the expression of IL-17 and MMP-9 in non-small cell cancer of the Han and Uighur NSCLC patients,analyze the differentiation of expression and the relationship between them and the clinical significance. Methods The expresion of IL-17 and MMP-9 were detected in 30 cases in Han and 30 cases in Uighur and lung tissues as control by PV9000 immunohistochemical technique. Results The positive expression of IL-17 and MMP-9 was detected in 60 cases NSCLC was 78.3% and 80.0%,respectively.Which was significantly higher than that in adjacent non-tumor tissues (P<0.05).The positive expression of IL-17 was related with the size of the tumor,TNM staging and also related to lymph node metastasis(P<0.05).The positive expression of MMP-9 was related with the size of tumor and TNM staging,but there was not correlated to nationality,age,gender,pathologic types and differentiation.The survival rate between the Han and Uighur is no sense in this study.The survival rate was different between NSCLC tissues with positive and negative IL-17 and MMP-9 expression (P<0.05). Conclusion The higher expression of IL-17 and MMP-9 in NSCLC is not the main factor about the different ratio of Han and Uighur in our hospital;IL-17 and MMP-9 may play important roles in regulating NSCLC invasion and metastasis.Over expression of IL-17 and MMP-9 could act as a reference to evaluate unfavorable prognosis of NSCLC.
Objective To investigate the expression of IL-17 and MMP-9 in non-small cell cancer of the Han and Uighur NSCLC patients,analyze the differentiation of expression and the relationship between them and the clinical significance. Methods The expresion of IL-17 and MMP-9 were detected in 30 cases in Han and 30 cases in Uighur and lung tissues as control by PV9000 immunohistochemical technique. Results The positive expression of IL-17 and MMP-9 was detected in 60 cases NSCLC was 78.3% and 80.0%,respectively.Which was significantly higher than that in adjacent non-tumor tissues (P<0.05).The positive expression of IL-17 was related with the size of the tumor,TNM staging and also related to lymph node metastasis(P<0.05).The positive expression of MMP-9 was related with the size of tumor and TNM staging,but there was not correlated to nationality,age,gender,pathologic types and differentiation.The survival rate between the Han and Uighur is no sense in this study.The survival rate was different between NSCLC tissues with positive and negative IL-17 and MMP-9 expression (P<0.05). Conclusion The higher expression of IL-17 and MMP-9 in NSCLC is not the main factor about the different ratio of Han and Uighur in our hospital;IL-17 and MMP-9 may play important roles in regulating NSCLC invasion and metastasis.Over expression of IL-17 and MMP-9 could act as a reference to evaluate unfavorable prognosis of NSCLC.
2012, 39(07): 802-806.
DOI: 10.3971/j.issn.1000-8578.2012.07.010
Abstract:
Objective To explore miRNAs that relative to tumorigenesis as biomarkers for early detecting nonsmoking female lung cancer of Xuanwei County. Methods (1) Detecting miRNAs expression in 37 cancer tissues in nonsmoking female lung cancer of Xuanwei County and 32 cancer tissues in individual cases by microarray hybridization.To validate the microarray analysis result by RT-qPCR.(2) Evaluating plasma expressions of the miRNAs in 16 stage Ⅰnonsmoking female lung cancer of Xuanwei County patients and 14 counterpart benign cases.Evaluating diagnostic sensitivity and specificity of the miRNA in plasma for nonsmoking female lung cancer of Xuanwei County by Receiver-Operator Characteristic curve(ROC). Results There were 31 miRNAs differentially expressed in all tumor tissues compared to their corresponding normal lung cancer.The over expressions of the genes in tumors were confirmed by RT-qPCR.The miR-21,miR-10a,miR-494,miR-22,miR-141 and miR-200b expression levels between two cancer tissues were different(P<0.05).The six miRNAs were stably present and reliably detectable in plasma.miR-494,miR-22 and miR -200b expression levels were significant different between two plasmas(P<0.01).The use of the three genes produced 85.26% sensitivity and 94.45% specificity in distinguishing nonsmoking female lung cancer of Xuanwei County patients from the female crowd. Conclusion From express stability,sensitivity and specificity that the identified three miRNAs provide potential biomarkers for early detection of nonsmoking female lung cancer of Xuanwei County.
Objective To explore miRNAs that relative to tumorigenesis as biomarkers for early detecting nonsmoking female lung cancer of Xuanwei County. Methods (1) Detecting miRNAs expression in 37 cancer tissues in nonsmoking female lung cancer of Xuanwei County and 32 cancer tissues in individual cases by microarray hybridization.To validate the microarray analysis result by RT-qPCR.(2) Evaluating plasma expressions of the miRNAs in 16 stage Ⅰnonsmoking female lung cancer of Xuanwei County patients and 14 counterpart benign cases.Evaluating diagnostic sensitivity and specificity of the miRNA in plasma for nonsmoking female lung cancer of Xuanwei County by Receiver-Operator Characteristic curve(ROC). Results There were 31 miRNAs differentially expressed in all tumor tissues compared to their corresponding normal lung cancer.The over expressions of the genes in tumors were confirmed by RT-qPCR.The miR-21,miR-10a,miR-494,miR-22,miR-141 and miR-200b expression levels between two cancer tissues were different(P<0.05).The six miRNAs were stably present and reliably detectable in plasma.miR-494,miR-22 and miR -200b expression levels were significant different between two plasmas(P<0.01).The use of the three genes produced 85.26% sensitivity and 94.45% specificity in distinguishing nonsmoking female lung cancer of Xuanwei County patients from the female crowd. Conclusion From express stability,sensitivity and specificity that the identified three miRNAs provide potential biomarkers for early detection of nonsmoking female lung cancer of Xuanwei County.
2012, 39(07): 807-810.
DOI: 10.3971/j.issn.1000-8578.2012.07.011
Abstract:
Objective To explore the relationship of abnormal expression of Skp2 and p27kip1 between intraductal proliferation lesions and breast cancer. Methods The positive expression ratio of Skp2 protein and p27kip1 protein in 30 cases of normal control group,120 cases of intraductal proliferation lesions including 30 cases of usual ductal hyperplasia (UDH),30 cases flat epithelial atypia(FEA),30 cases of atypical ductal hyperplasia(ADH) and 30 cases of ductal carcinoma in situ(DCIS) were detected using immunohistochemical method. Results The positive expression ratio of Skp2 in four subtypes were all higher than that in normal control (P<0.05).The positive expression ratio of Skp2 protein of DCIS,FEA and ADH were higher than that of UDH.There were significant difference between DCIS,FEA and ADH(P<0.05).The positive expression ratio of p27Kip1 in four subtypes were all lower than that in normal control (P<0.05).Remarkable difference of expression ratio of p27kip1 existed between UDH and DCIS (P<0.05),FEA and DCIS (P<0.05),ADH and DCIS (P<0.05).Skp2 protein level was reversely correlated with p27kip1 protein level overall(r=-0.411,P=0.000).The same results existed in UDH and DCIS,respectively(r=-0.406,P=0.026;r=-0.544,P=0.002). Conclusion The increase of expression level of Skp2 protein and decrease of expression level of p27kip1 protein have closed association with dysplasia of breast ductal epithelial and development of breast cancer.
Objective To explore the relationship of abnormal expression of Skp2 and p27kip1 between intraductal proliferation lesions and breast cancer. Methods The positive expression ratio of Skp2 protein and p27kip1 protein in 30 cases of normal control group,120 cases of intraductal proliferation lesions including 30 cases of usual ductal hyperplasia (UDH),30 cases flat epithelial atypia(FEA),30 cases of atypical ductal hyperplasia(ADH) and 30 cases of ductal carcinoma in situ(DCIS) were detected using immunohistochemical method. Results The positive expression ratio of Skp2 in four subtypes were all higher than that in normal control (P<0.05).The positive expression ratio of Skp2 protein of DCIS,FEA and ADH were higher than that of UDH.There were significant difference between DCIS,FEA and ADH(P<0.05).The positive expression ratio of p27Kip1 in four subtypes were all lower than that in normal control (P<0.05).Remarkable difference of expression ratio of p27kip1 existed between UDH and DCIS (P<0.05),FEA and DCIS (P<0.05),ADH and DCIS (P<0.05).Skp2 protein level was reversely correlated with p27kip1 protein level overall(r=-0.411,P=0.000).The same results existed in UDH and DCIS,respectively(r=-0.406,P=0.026;r=-0.544,P=0.002). Conclusion The increase of expression level of Skp2 protein and decrease of expression level of p27kip1 protein have closed association with dysplasia of breast ductal epithelial and development of breast cancer.
2012, 39(07): 811-817.
DOI: 10.3971/j.issn.1000-8578.2012.07.012
Abstract:
Objective To establish the overall diagnostic accuracy of the measurements of serum squamous cell carcinoma antigen (SCC-Ag) for lymph node metastasis(LNM) in squamous cell cervical cancer. Methods After a systematic review of current studies,we pooled the sensitivity,specificity of serum SCC-Ag in diagnosis LNM cases from cervical cancer patient using random effects models.Qualified studies were retrieved from The Cochrane Library,Cochrane Central Register of Controlled Trials,Pubmed,Embase,CNKI,WanFang,and CBM database.Two reviewers independently assessed the methodological quality of each study with the tool of QUADAS.We drew the Summary Receiver Operating Characteristic (SROC) curve、funnel figure of the included studies and made Meta-analyses using Meta-Disc 1.4 and Stata 11.0 software. Results Ten studies met the inclusion criteria for the analysis.After testing the heterogeneity of the included studies,a random effect model was selected to calculate the pool weighted sensitivity and specificity with 95% confidence interval:the sensitivity was 60%[95%CI(0.54~0.65)],the specificity was76%[95%CI(0.73~0.78)],the DOR was 5.38[95%CI(3.27~8.87)],and the AUC of SROC was 0.7126(unsatisfied).The sources of heterogeneity maybe according to the cutoff. Conclusion SCC-Ag has certain diagnosis value in LNM cases,but it is not a perfect index to identify LNM cases from cervical cancer patients.
Objective To establish the overall diagnostic accuracy of the measurements of serum squamous cell carcinoma antigen (SCC-Ag) for lymph node metastasis(LNM) in squamous cell cervical cancer. Methods After a systematic review of current studies,we pooled the sensitivity,specificity of serum SCC-Ag in diagnosis LNM cases from cervical cancer patient using random effects models.Qualified studies were retrieved from The Cochrane Library,Cochrane Central Register of Controlled Trials,Pubmed,Embase,CNKI,WanFang,and CBM database.Two reviewers independently assessed the methodological quality of each study with the tool of QUADAS.We drew the Summary Receiver Operating Characteristic (SROC) curve、funnel figure of the included studies and made Meta-analyses using Meta-Disc 1.4 and Stata 11.0 software. Results Ten studies met the inclusion criteria for the analysis.After testing the heterogeneity of the included studies,a random effect model was selected to calculate the pool weighted sensitivity and specificity with 95% confidence interval:the sensitivity was 60%[95%CI(0.54~0.65)],the specificity was76%[95%CI(0.73~0.78)],the DOR was 5.38[95%CI(3.27~8.87)],and the AUC of SROC was 0.7126(unsatisfied).The sources of heterogeneity maybe according to the cutoff. Conclusion SCC-Ag has certain diagnosis value in LNM cases,but it is not a perfect index to identify LNM cases from cervical cancer patients.
2012, 39(07): 818-821.
DOI: 10.3971/j.issn.1000-8578.2012.07.013
Abstract:
Objective To investigate tumor regression in patients with intact cervical cancer. Methods We analyzed 20 patients undergoing intensity-modulated radiation therapy,scanning CT image before and after radiotherapy to study tumor regression. Results Data show that CTV average regression is 17.3%,GTVtumor average regression is 48.2%,and there is influence on adjacent organs. Conclusion Obvious regression of CTV and GTV tumor in radiotherapy is observed.Individual patients with large GTV tumor,should be closely observe its regression and impact to adjacent organs,if necessary,should re-create the treatment plan.
Objective To investigate tumor regression in patients with intact cervical cancer. Methods We analyzed 20 patients undergoing intensity-modulated radiation therapy,scanning CT image before and after radiotherapy to study tumor regression. Results Data show that CTV average regression is 17.3%,GTVtumor average regression is 48.2%,and there is influence on adjacent organs. Conclusion Obvious regression of CTV and GTV tumor in radiotherapy is observed.Individual patients with large GTV tumor,should be closely observe its regression and impact to adjacent organs,if necessary,should re-create the treatment plan.
Association of Variant Allele of DICER and Decreased Risk of Esophageal Cancer Lymph Node Metastasis
2012, 39(07): 822-825.
DOI: 10.3971/j.issn.1000-8578.2012.07.014
Abstract:
Objective To investigate the relationship between DICER polymorphism and the susceptibility to lymph node metastasis in esophageal cancer in Chinese Han population. Methods Genotypes were determined by matrix-assisted laser desorption /ionization time-of-flight mass spectrometry (MALDI-TOF MS) method in 85 lymph node metastasis positive patients and 270 lymph node metastasis negative patients. Results The DICER rs3742330 A>G genotype frequencies were 31.76%(AA),61.18%(AG),7.06%(GG) in the lymph node positive group and 34.44%(AA),48.15%(AG),17.41%(GG) in the lymph node negative group,respectively.Logistic regression analyses revealed that the risk associated with DICER rs3742330 GG genotype was 0.36 (95%CI=0.15~0.88) which was much lower than DICER rs3742330 AA+AG genotype. Conclusion DICER rs3742330 A>G polymorphism may be a protective factor of lymph node metastasis.
Objective To investigate the relationship between DICER polymorphism and the susceptibility to lymph node metastasis in esophageal cancer in Chinese Han population. Methods Genotypes were determined by matrix-assisted laser desorption /ionization time-of-flight mass spectrometry (MALDI-TOF MS) method in 85 lymph node metastasis positive patients and 270 lymph node metastasis negative patients. Results The DICER rs3742330 A>G genotype frequencies were 31.76%(AA),61.18%(AG),7.06%(GG) in the lymph node positive group and 34.44%(AA),48.15%(AG),17.41%(GG) in the lymph node negative group,respectively.Logistic regression analyses revealed that the risk associated with DICER rs3742330 GG genotype was 0.36 (95%CI=0.15~0.88) which was much lower than DICER rs3742330 AA+AG genotype. Conclusion DICER rs3742330 A>G polymorphism may be a protective factor of lymph node metastasis.
2012, 39(07): 826-828.
DOI: 10.3971/j.issn.1000-8578.2012.07.015
Abstract:
Objective To analyze the clinicopathological characteristics of patients with SRC in comparison with non- signet ring cell carcinoma(NSRC) of the stomach. Methods Retrospective study on the clinicopathological characteristics of 110 patients with SRC and 326 patients with NSRC. Results In this study,the proportion of men and women in SRC (1.4∶1) is lower than in the NSRC group (2.5∶1)(P<0.01);among all the patients younger than 60 years old,the morbidity of SRC(43.6%) is higher than that of NSRC(30.9%) (P<0.05);the proportionality of SRC is 31.4% of all the patients with gastric cancer in stage Ⅰ,and 23.3% in the other stages(P<0.05);the proportion of the tumor which infiltrate into submucosa is lower in the SRC group(70%)than the NSRC group(86.9%)(P<0.001);but no significant differences in tumor location or lymph node metastasis between two groups(SRC and NSRC). Conclusion Signet ring cell carcinoma happens more commom in young females and the incidence rate is higher in early carcinoma and infiltrates more slowly in submucosa than mucosa.
Objective To analyze the clinicopathological characteristics of patients with SRC in comparison with non- signet ring cell carcinoma(NSRC) of the stomach. Methods Retrospective study on the clinicopathological characteristics of 110 patients with SRC and 326 patients with NSRC. Results In this study,the proportion of men and women in SRC (1.4∶1) is lower than in the NSRC group (2.5∶1)(P<0.01);among all the patients younger than 60 years old,the morbidity of SRC(43.6%) is higher than that of NSRC(30.9%) (P<0.05);the proportionality of SRC is 31.4% of all the patients with gastric cancer in stage Ⅰ,and 23.3% in the other stages(P<0.05);the proportion of the tumor which infiltrate into submucosa is lower in the SRC group(70%)than the NSRC group(86.9%)(P<0.001);but no significant differences in tumor location or lymph node metastasis between two groups(SRC and NSRC). Conclusion Signet ring cell carcinoma happens more commom in young females and the incidence rate is higher in early carcinoma and infiltrates more slowly in submucosa than mucosa.
2012, 39(07): 829-832.
DOI: 10.3971/j.issn.1000-8578.2012.07.016
Abstract:
Objective To discuss the clinical features and outcome of malignant melanoma of the skin on foot. Methods From 1998 to 2010,61 patients with melanoma on foot were retrospectively analyzed in our department.The main treatments were surgery,chemotherapy and immunotherapy.All patients undergo operation,all patients were treated by toe resection,lesion resection and skin graft,or lesions resection with flap transfer.while 32 cases had groin lymph node reaction at the same time.The survival rate was statistics.Factors that might be related to survival,included tumor depth,ulcer and lymph node metastasis.Analyzed by Kaplan-Meier and Log-rank method. Results Fifty-three patients were followed,and 8 of them were lost to follow up 2 years later.The follow up period ranged from 1 to 10 years,4.5 years on average.Survival rates in 1,3 and 5 years were 77.0%(47/61),58.5%(31/53) and 26.4%(14/53),respectively.The appearance and function of foot recovered well,and the local recurrence didn't appear.Among them,27 patients died from systemic metastasis,and regional groin lymph node recurrence appeared in twelve patients. Conclusion The rate of metastasis in patients with melanoma on foot was higher.Factors related to survival,included tumor ulcer and depth,and lymph node metastasis,are analyzed by Kaplan-Meier and Log-rank method,the tumor depth may play a more important role in operation.Combination therapy included surgery,chemotherapy,and immunotherapy is the main treatment.
Objective To discuss the clinical features and outcome of malignant melanoma of the skin on foot. Methods From 1998 to 2010,61 patients with melanoma on foot were retrospectively analyzed in our department.The main treatments were surgery,chemotherapy and immunotherapy.All patients undergo operation,all patients were treated by toe resection,lesion resection and skin graft,or lesions resection with flap transfer.while 32 cases had groin lymph node reaction at the same time.The survival rate was statistics.Factors that might be related to survival,included tumor depth,ulcer and lymph node metastasis.Analyzed by Kaplan-Meier and Log-rank method. Results Fifty-three patients were followed,and 8 of them were lost to follow up 2 years later.The follow up period ranged from 1 to 10 years,4.5 years on average.Survival rates in 1,3 and 5 years were 77.0%(47/61),58.5%(31/53) and 26.4%(14/53),respectively.The appearance and function of foot recovered well,and the local recurrence didn't appear.Among them,27 patients died from systemic metastasis,and regional groin lymph node recurrence appeared in twelve patients. Conclusion The rate of metastasis in patients with melanoma on foot was higher.Factors related to survival,included tumor ulcer and depth,and lymph node metastasis,are analyzed by Kaplan-Meier and Log-rank method,the tumor depth may play a more important role in operation.Combination therapy included surgery,chemotherapy,and immunotherapy is the main treatment.
2012, 39(07): 833-837.
DOI: 10.3971/j.issn.1000-8578.2012.07.017
Abstract:
Objective To detect overexpression of ER,PR,HER-2 by TLC in breast cancer,compare,the results of immunocytochemistry (ICC) with that of immunohistochemistry (IHC),and observe their available in advanced breast cancer. Methods The methods of ICC and IHC were used in breast cancer specimens. Results The satisfied slides for ER,PR and HER-2 were 195,194 and 202 cases in all 205 cases,respectirely.There were 2 exfoliative slides.They occupied 1.0%.One hundred and seventy cases have histopathology results.The positive expression rate of ER was 60.0% in IHC,58.5% in ICC (P=0.765),PR was 70.6% in IHC,59.3% in ICC(P<0.05),and HER-2 was 57.5% in IHC,38.1% in ICC (P<0.05).The patients were mostly in the age group 51~61years old (59 cases,32.3%).50.8% patients had lymph node metastasis in this group.47.1% patients had lymph node metastasis in 40~50 years old age group.14 out of 16 cases had lymph node metastasis in 29~39 years old age group.They occupied 87.5%.ER+/PR+/HER-2- overexpression were the highest in 73 years old age group,and the lowest in 29~39 years group.This was consistant with clinical feature and cytologic morphology.The results of assessment of FNA specimens agreed with those of the histological sections in 150 of the 160 cases in ER.Their sensitivity,specificity,positivity predictive value,negative predictive value,false positive rate,false negative rate were 94.1%,93.2%,96.0%,90.2%,2.5% and 3.8%,respectively.The results of assessment of FNA specimens agreed with those of the histological sections in 140 of the 159 cases in PR.Their sensitivity,specificity,positivity predictive value,negative predictive value,false positive rate,false negative rate were 88.5%,87.0%,94.3%,75.5%,3.8%,and 8.2%,respectively.The results of assessment of FNA specimens agreed with those of the histological sections in 134 of the 167 cases in HER-2.Their sensitivity,specificity,positivity predictive value,negative predictive value,false positive rate,false negative rate were 67.7%,97.2%,97.0%,69.0%,1.2% and 18.6%,respectively. Conclusion The results of assessment of ICC have a good correlation with that of IHC.When only cytologic material might be available,it would be useful to be able to identify ER,PR status by using FNA specimens.The intratumoral heterogeneity of positive cells was the main reason for discordant results between cytological and histologic specimens.HER-2 diagnosis standard of ICC should not be the same as that of IHC in HER-2 test.Her-2 overexpression can be tested in FNA specimens,whereas positive HER-2 staining on TLC would require further validation by FISH.
Objective To detect overexpression of ER,PR,HER-2 by TLC in breast cancer,compare,the results of immunocytochemistry (ICC) with that of immunohistochemistry (IHC),and observe their available in advanced breast cancer. Methods The methods of ICC and IHC were used in breast cancer specimens. Results The satisfied slides for ER,PR and HER-2 were 195,194 and 202 cases in all 205 cases,respectirely.There were 2 exfoliative slides.They occupied 1.0%.One hundred and seventy cases have histopathology results.The positive expression rate of ER was 60.0% in IHC,58.5% in ICC (P=0.765),PR was 70.6% in IHC,59.3% in ICC(P<0.05),and HER-2 was 57.5% in IHC,38.1% in ICC (P<0.05).The patients were mostly in the age group 51~61years old (59 cases,32.3%).50.8% patients had lymph node metastasis in this group.47.1% patients had lymph node metastasis in 40~50 years old age group.14 out of 16 cases had lymph node metastasis in 29~39 years old age group.They occupied 87.5%.ER+/PR+/HER-2- overexpression were the highest in 73 years old age group,and the lowest in 29~39 years group.This was consistant with clinical feature and cytologic morphology.The results of assessment of FNA specimens agreed with those of the histological sections in 150 of the 160 cases in ER.Their sensitivity,specificity,positivity predictive value,negative predictive value,false positive rate,false negative rate were 94.1%,93.2%,96.0%,90.2%,2.5% and 3.8%,respectively.The results of assessment of FNA specimens agreed with those of the histological sections in 140 of the 159 cases in PR.Their sensitivity,specificity,positivity predictive value,negative predictive value,false positive rate,false negative rate were 88.5%,87.0%,94.3%,75.5%,3.8%,and 8.2%,respectively.The results of assessment of FNA specimens agreed with those of the histological sections in 134 of the 167 cases in HER-2.Their sensitivity,specificity,positivity predictive value,negative predictive value,false positive rate,false negative rate were 67.7%,97.2%,97.0%,69.0%,1.2% and 18.6%,respectively. Conclusion The results of assessment of ICC have a good correlation with that of IHC.When only cytologic material might be available,it would be useful to be able to identify ER,PR status by using FNA specimens.The intratumoral heterogeneity of positive cells was the main reason for discordant results between cytological and histologic specimens.HER-2 diagnosis standard of ICC should not be the same as that of IHC in HER-2 test.Her-2 overexpression can be tested in FNA specimens,whereas positive HER-2 staining on TLC would require further validation by FISH.
2012, 39(07): 838-840.
DOI: 10.3971/j.issn.1000-8578.2012.07.018
Abstract:
Objective To evaluate the safety and efficacy of neoadjuvant chemoradiation for elderly patients (age≥70) with local advanced esophageal squamous carcinomas. Methods A total 27 elder patients staged cT3N0~1(group A) were analyzed retrospectively compared with 62 cases of younger patients(group B),all of them were given 2 cycles cisplatin 75 mg/m2 plus fluorouracil 600 mg/m2 d1~5 and 40 Gy of radiation followed by surgery 3~4 weeks later in past two years. Results All patients accomplished planned neoadjuvant chemoradiations.Neoadjuvant chemoradiation caused vomiting,neutropenia and esophagitis in two arms similarly,only G3/4 neutropenia occurred in 25.9%(7/27) of group A versus 8.1%(5/62) of group B (P=0.023).The effective rate of neoadjuvant chemoradiation was 92.6%(25/27) in group A vs.95.2% in group B (P=0.621).88.9% patients (24/27)underwent complete resection of group A and 87.1%(54/62) of group B (P=0.563).No mortality was found.The overall complication rate were similar in two groups (32.0% vs.22.0%).Arrhythmia,pneumonia and injury of recurrent laryngeal nerve were the common complications after surgery.The incidence of arrhythmia in group A occurs was 32.0% contrast to 8.5% in group B (P=0.015).There was no difference of pCR between two groups (25.9% vs.22.6%). Conclusion There was no significant difference between elderly patients and younger patients with local advanced esophageal squamous carcinomas treated by neoadjuvant chemoradiation followed by surgery,though increased G3/4 neutropenias and arrhythmias.The neoadjuvant chemoradiation was safety and effective for elderly patients.
Objective To evaluate the safety and efficacy of neoadjuvant chemoradiation for elderly patients (age≥70) with local advanced esophageal squamous carcinomas. Methods A total 27 elder patients staged cT3N0~1(group A) were analyzed retrospectively compared with 62 cases of younger patients(group B),all of them were given 2 cycles cisplatin 75 mg/m2 plus fluorouracil 600 mg/m2 d1~5 and 40 Gy of radiation followed by surgery 3~4 weeks later in past two years. Results All patients accomplished planned neoadjuvant chemoradiations.Neoadjuvant chemoradiation caused vomiting,neutropenia and esophagitis in two arms similarly,only G3/4 neutropenia occurred in 25.9%(7/27) of group A versus 8.1%(5/62) of group B (P=0.023).The effective rate of neoadjuvant chemoradiation was 92.6%(25/27) in group A vs.95.2% in group B (P=0.621).88.9% patients (24/27)underwent complete resection of group A and 87.1%(54/62) of group B (P=0.563).No mortality was found.The overall complication rate were similar in two groups (32.0% vs.22.0%).Arrhythmia,pneumonia and injury of recurrent laryngeal nerve were the common complications after surgery.The incidence of arrhythmia in group A occurs was 32.0% contrast to 8.5% in group B (P=0.015).There was no difference of pCR between two groups (25.9% vs.22.6%). Conclusion There was no significant difference between elderly patients and younger patients with local advanced esophageal squamous carcinomas treated by neoadjuvant chemoradiation followed by surgery,though increased G3/4 neutropenias and arrhythmias.The neoadjuvant chemoradiation was safety and effective for elderly patients.
2012, 39(07): 841-843.
DOI: 10.3971/j.issn.1000-8578.2012.07.019
Abstract:
Objective To assess the effects of angioplasty double gastric emptying barrier on stomach esophagus, the anastomosis, narrow and the flowing esophagitis. Methods Patients cured by radical resection of cardiaoesophagus carcinoma was used for control group.The treatment group: patients were treated by radical resection of cardiaoesophagus carcinoma combined with double angioplasty. Results In treatment group, one patient appears gastric emptying obstacles, one is flowing esophagitis, one is anastomotic fistula, and the percentage was 0.43%, repectively.Nine cases appear anastomotic stenosis, about 3.90%; In control group, gastric emptying obstacles is 8 cases, about 3.57%, the flowing esophagitis is 7 patients, about 3.13%, anastomosis (9 cases, about 4.02%, anastomotic stenosis is 10 cases, about 4.46%. Two groups of comparisons gastric emptying disorders, the flowing esophagitis and anastomosis likely to have a significant difference (P<0.05), anastomotic stenosis no significant difference of chance (P>0.05). ConclusionDouble angioplasty can effectively prevent esophageal angioplasty after stomach stapling gastric emptying, reduce barriers esophageal stomach anastomosis and the flowing esophagitis happening, decrease the incidence of anastomosis stricture.
Objective To assess the effects of angioplasty double gastric emptying barrier on stomach esophagus, the anastomosis, narrow and the flowing esophagitis. Methods Patients cured by radical resection of cardiaoesophagus carcinoma was used for control group.The treatment group: patients were treated by radical resection of cardiaoesophagus carcinoma combined with double angioplasty. Results In treatment group, one patient appears gastric emptying obstacles, one is flowing esophagitis, one is anastomotic fistula, and the percentage was 0.43%, repectively.Nine cases appear anastomotic stenosis, about 3.90%; In control group, gastric emptying obstacles is 8 cases, about 3.57%, the flowing esophagitis is 7 patients, about 3.13%, anastomosis (9 cases, about 4.02%, anastomotic stenosis is 10 cases, about 4.46%. Two groups of comparisons gastric emptying disorders, the flowing esophagitis and anastomosis likely to have a significant difference (P<0.05), anastomotic stenosis no significant difference of chance (P>0.05). ConclusionDouble angioplasty can effectively prevent esophageal angioplasty after stomach stapling gastric emptying, reduce barriers esophageal stomach anastomosis and the flowing esophagitis happening, decrease the incidence of anastomosis stricture.
2012, 39(07): 844-846.
DOI: 10.3971/j.issn.1000-8578.2012.07.020
Abstract:
Objective To study the clinical effect of Yanshu on primary hepatic carcinoma treated with interventional therapy. Methods A total of 82 patients with primary hepatic carcinoma were randomly divided into the treatment group(42 cases) and control group(40 cases). All patients concurrently received TACE,and the treatment group was additionally treated with Yanshu injection. The curative effects, survival period, clinical symptoms and toxic reactions were observed. Results There was no significant difference between two groups in total effective rates(P<0.05). However, there were significant differences in 24-months-survival-rates, clinical symptoms and toxic reactions between two groups(P<0.05). Conclusion TACE in combination with Yanshu can protect liver function, improve clinical symptoms, reduce toxic effects and prolong survival of patients.
Objective To study the clinical effect of Yanshu on primary hepatic carcinoma treated with interventional therapy. Methods A total of 82 patients with primary hepatic carcinoma were randomly divided into the treatment group(42 cases) and control group(40 cases). All patients concurrently received TACE,and the treatment group was additionally treated with Yanshu injection. The curative effects, survival period, clinical symptoms and toxic reactions were observed. Results There was no significant difference between two groups in total effective rates(P<0.05). However, there were significant differences in 24-months-survival-rates, clinical symptoms and toxic reactions between two groups(P<0.05). Conclusion TACE in combination with Yanshu can protect liver function, improve clinical symptoms, reduce toxic effects and prolong survival of patients.
2012, 39(07): 847-850.
DOI: 10.3971/j.issn.1000-8578.2012.07.021
Abstract:
Objective To compare the clinical efficacy and toxicity of paclitaxel versus 5-fluorouracil (5-Fu) combined with carboplatin for the treatment in patients with advanced penile carcinoma associated with AIDS. Methods Fifty-eight patients were enrolled in this study,these patients with advanced penile carcinoma and infected by HIV which were confirmed by serologic biopsy and cytology evidence.Thirty patients in the paclitaxel and carboplatin group(TC group) administered paclitaxel at a dose of 175 mg/m2 given intravenously on day one,carboplatin at a dose of AUC=5 given intravenously on day one every 21 days cycle.Twenty-eight patients in 5-Fu and carboplatin group(FC group) administered 5-Fu at a dose of 1 000 mg/m2 given intravenously on four consecutive days,carboplatin at a dose of AUC=5 given intravenously on day one every 21 days cycle. Results All the 58 patients were assessable for response.The objective overall response rate was 56.7% in paclitaxel and carboplatin group and 17.9% in 5-Fu and carboplatin group,respectively(χ2=9.265,P=0.002).The median time to disease progression was 11.15 months vs.7.22 months (χ2=6.737,P=0.009).The median duration of response was 17.36 months vs.9.11 months (χ2=10.646,P=0.001).The most common toxicity was myelosuppression,nausea,vomit.The rates of grade Ⅲ~Ⅳ leucopenia were 46.4% and 40.0% (χ2=0.222,P=0.637).The rates of grade Ⅲ~Ⅴ nausea and vomit were 36.0% and 34.78% (χ2=0.008,P=0.930). Conclusion sCombination chemotherapy with paclitaxel and carboplatin is an effective and safe regimen in the treatment for patients with advanced penile carcinoma associated with AIDS.
Objective To compare the clinical efficacy and toxicity of paclitaxel versus 5-fluorouracil (5-Fu) combined with carboplatin for the treatment in patients with advanced penile carcinoma associated with AIDS. Methods Fifty-eight patients were enrolled in this study,these patients with advanced penile carcinoma and infected by HIV which were confirmed by serologic biopsy and cytology evidence.Thirty patients in the paclitaxel and carboplatin group(TC group) administered paclitaxel at a dose of 175 mg/m2 given intravenously on day one,carboplatin at a dose of AUC=5 given intravenously on day one every 21 days cycle.Twenty-eight patients in 5-Fu and carboplatin group(FC group) administered 5-Fu at a dose of 1 000 mg/m2 given intravenously on four consecutive days,carboplatin at a dose of AUC=5 given intravenously on day one every 21 days cycle. Results All the 58 patients were assessable for response.The objective overall response rate was 56.7% in paclitaxel and carboplatin group and 17.9% in 5-Fu and carboplatin group,respectively(χ2=9.265,P=0.002).The median time to disease progression was 11.15 months vs.7.22 months (χ2=6.737,P=0.009).The median duration of response was 17.36 months vs.9.11 months (χ2=10.646,P=0.001).The most common toxicity was myelosuppression,nausea,vomit.The rates of grade Ⅲ~Ⅳ leucopenia were 46.4% and 40.0% (χ2=0.222,P=0.637).The rates of grade Ⅲ~Ⅴ nausea and vomit were 36.0% and 34.78% (χ2=0.008,P=0.930). Conclusion sCombination chemotherapy with paclitaxel and carboplatin is an effective and safe regimen in the treatment for patients with advanced penile carcinoma associated with AIDS.
2012, 39(07): 851-854.
DOI: 10.3971/j.issn.1000-8578.2012.07.022
Abstract:
Objective To evaluate the clinical efficacy and safety of paclitaxel plus cisplatin chemotherapy combining concurrent radiotherapy on esophageal carcinoma. Methods We searched PubMed,EMBASE,Cochrane Library,CNKI,and other databases to collect randomized controlled trials (RCTs).According to the statistical analysis of RevMan5.0 statistical software. ResultsThe results were included in six randomized controlled trials,including 559 cases of patients.Meta analysis showed that in the treatment of advanced esophageal carcinoma,when compared with radiotherapy alone,the concurrent radiotherapy and chemotherapy can improve the efficiency,the RR value and 95% confidence interval were 1.28(1.17,1.40) and 1,2 or 3-year survival rate of RR values and 95% confidence interval are 1.25 (1.10,1.42),1.44(1.18,1.76),1.46(1.15,1.86),respectively.However,the toxicities also increased,such as the incidence of radioactive esophagitis and gastrointestinal adverse reactions were 2.49(1.02,6.08),25.00 (3.49,179.25),respectively. Conclusion The efficacy of pacli taxol plus cisplatin combined with concurrent radiotherapy on esophageal carcinoma is more effective than that of concurrent radiotherapy alone.Although the adverse reactions are increased at the same time,most of them can be tolerated.
Objective To evaluate the clinical efficacy and safety of paclitaxel plus cisplatin chemotherapy combining concurrent radiotherapy on esophageal carcinoma. Methods We searched PubMed,EMBASE,Cochrane Library,CNKI,and other databases to collect randomized controlled trials (RCTs).According to the statistical analysis of RevMan5.0 statistical software. ResultsThe results were included in six randomized controlled trials,including 559 cases of patients.Meta analysis showed that in the treatment of advanced esophageal carcinoma,when compared with radiotherapy alone,the concurrent radiotherapy and chemotherapy can improve the efficiency,the RR value and 95% confidence interval were 1.28(1.17,1.40) and 1,2 or 3-year survival rate of RR values and 95% confidence interval are 1.25 (1.10,1.42),1.44(1.18,1.76),1.46(1.15,1.86),respectively.However,the toxicities also increased,such as the incidence of radioactive esophagitis and gastrointestinal adverse reactions were 2.49(1.02,6.08),25.00 (3.49,179.25),respectively. Conclusion The efficacy of pacli taxol plus cisplatin combined with concurrent radiotherapy on esophageal carcinoma is more effective than that of concurrent radiotherapy alone.Although the adverse reactions are increased at the same time,most of them can be tolerated.
2012, 39(07): 855-859.
DOI: 10.3971/j.issn.1000-8578.2012.07.023
Abstract:
Objective To explore the effect of the informed status at different levels on the quality of life in lung cancer patients by survey. Methods The information was collected by questionnaire in lung cancer patients from June 2007 to December 2007.The FACT-L scale which was specialized for the evaluation of quality of life for lung cancer patients was used in this study. Results One hundred and fifteen patients were enrolled in this study,of those patients,the totally informed,partially informed and not informed patients account for 31.3%,49.6% and 19.1% respectively.The result indicated that different informed status significantly,affect the social/familial status and performance status(P value is 0.001 and 0.042 individually),and totally informed patients have obviously better social/familial status than partially informed and not informed patients.Further analysis showed that not informed patients have less influence on the social/familial life than that on the partially informed and totally informed patients (P value is 0.027 and 0.001 individually),the evaluation score in totally informed patients is higher than partially informed patients,but it failed to reach statistical significance (P=0.266). Conclusion At present,the percentage of informed hospitalized lung cancer patients is only 31.3% now days.Better informed status has positive influence on social/familial status.Thus,it is better to inform the patients of their disease at a suitable situation.
Objective To explore the effect of the informed status at different levels on the quality of life in lung cancer patients by survey. Methods The information was collected by questionnaire in lung cancer patients from June 2007 to December 2007.The FACT-L scale which was specialized for the evaluation of quality of life for lung cancer patients was used in this study. Results One hundred and fifteen patients were enrolled in this study,of those patients,the totally informed,partially informed and not informed patients account for 31.3%,49.6% and 19.1% respectively.The result indicated that different informed status significantly,affect the social/familial status and performance status(P value is 0.001 and 0.042 individually),and totally informed patients have obviously better social/familial status than partially informed and not informed patients.Further analysis showed that not informed patients have less influence on the social/familial life than that on the partially informed and totally informed patients (P value is 0.027 and 0.001 individually),the evaluation score in totally informed patients is higher than partially informed patients,but it failed to reach statistical significance (P=0.266). Conclusion At present,the percentage of informed hospitalized lung cancer patients is only 31.3% now days.Better informed status has positive influence on social/familial status.Thus,it is better to inform the patients of their disease at a suitable situation.