2012 Vol. 39 No. 05
2012, 39(05): 493-496.
DOI: 10.3971/j.issn.1000-8578.2012.05.001
Abstract：
Objective To investigate the inhibitory effect of small interfering RNA on the expression of CD147 and invasion in human thyroid carcinoma cell line K1.And to screen the effective siRNA sequence. Methods Three pairs sequence of CD147-siRNA small interfering RNA designed and synthesized and were transfected into K1 cells to knockdown the CD147 expression.The mRNA and protein levels of CD147 were detected by RT-PCR and ELISA.The mRNA and protein levels of MMP7 were detected by RT-PCR and Western blot.Transwell chambers were used to detect the invasiveness ability of K1 cells in vitro. Results Compared with the normal group and control group,mRNA and protein level of CD147 in S1 group had no significant difference(P>0.05).In contrast,mRNA and protein level of CD147 decreased significantly(P<0.05) in the S2 and S3 groups,in which mRNA level decreased to 67.81% and 72.48%, respectively;and protein level decreased to 31.65% and 35.47%,respectively.Meanwhile,compared with the normal group and control group,mRNA and protein level of MMP7 in the S2 and S3 groups also decreased significantly，in which mRNA level decreased to 50.25% and 53.40%, respectively,and protein level decreased to 41.58% and 40.49%,respectively.In transwell chamber assay,after transfection 72 h,the invasion inhibition rate of the cells went through membrane in the S2 and S3 groups was 35.87% and 30.16%,respectively,which was less than thatin the S1 group.While there were no significant difference(P>0.05) among the S1,normal and control groups in the results from Transwell assay mentioned above. Conclusion CD147-siRNA in the S2 and S3 groups can decrease the expression of CD147，resulting in the suppression of invasiveness activity of tumor in vitro.The use of CD147 specific siRNA deserves further investigation as a novel approach to cancer therapy in the future.
Objective To investigate the inhibitory effect of small interfering RNA on the expression of CD147 and invasion in human thyroid carcinoma cell line K1.And to screen the effective siRNA sequence. Methods Three pairs sequence of CD147-siRNA small interfering RNA designed and synthesized and were transfected into K1 cells to knockdown the CD147 expression.The mRNA and protein levels of CD147 were detected by RT-PCR and ELISA.The mRNA and protein levels of MMP7 were detected by RT-PCR and Western blot.Transwell chambers were used to detect the invasiveness ability of K1 cells in vitro. Results Compared with the normal group and control group,mRNA and protein level of CD147 in S1 group had no significant difference(P>0.05).In contrast,mRNA and protein level of CD147 decreased significantly(P<0.05) in the S2 and S3 groups,in which mRNA level decreased to 67.81% and 72.48%, respectively;and protein level decreased to 31.65% and 35.47%,respectively.Meanwhile,compared with the normal group and control group,mRNA and protein level of MMP7 in the S2 and S3 groups also decreased significantly，in which mRNA level decreased to 50.25% and 53.40%, respectively,and protein level decreased to 41.58% and 40.49%,respectively.In transwell chamber assay,after transfection 72 h,the invasion inhibition rate of the cells went through membrane in the S2 and S3 groups was 35.87% and 30.16%,respectively,which was less than thatin the S1 group.While there were no significant difference(P>0.05) among the S1,normal and control groups in the results from Transwell assay mentioned above. Conclusion CD147-siRNA in the S2 and S3 groups can decrease the expression of CD147，resulting in the suppression of invasiveness activity of tumor in vitro.The use of CD147 specific siRNA deserves further investigation as a novel approach to cancer therapy in the future.
2012, 39(05): 497-501.
DOI: 10.3971/j.issn.1000-8578.2012.05.002
Abstract：
Objective To study the effects of Xiaoaiping(XAP) with different concentrations on the tumor angiogenesis in mice and to discuss its possible mechanism in the inhibition of hepatic cellular cancer growth. Methods Seventy-five female KM mice whose weight is about(20±2)g were injected H22 liver tumor cells.They were randomly divided into five groups as following:D1(5-fluorouracil(5-Fu) was peritoneal injected,as a positive control),D2(NS was peritoneal injected,as a negative control),groups X1,X2 and X3 were peritoneal injected with XAP of 10 g/(kg·d),20 g/(kg·d) and 40 g/(kg ·d) respectively.The tumor′s volume was calculated to draw the tumor growth curve.The tissue of xenografted tumors was taken after 3 week′s treatment.The xenografted tumors were weighted its inhibitory rate was calculated.The pathological changes were observed by light microscope.The microvessel density(MVD) marked by CD34 in the xenografted tumor tissue was detected by immunohistochemisty.The concentration of serum VEGF was measured by ELISA. Results The inhibition rate in X3 and D1 group was similar(P>0.05).The doses of serum VEGF in X1,X2 and X3 group were all lower than that in D2 group(P<0.05),especially in X3group.To detect MVD,the conditions were the same as the dose of serum VEGF. Conclusion Different doses of XAP can inhibit tumor growth of H22 liver cancer in mice and the medium dose of XAP has the best inhibition.XAP may reduce VEGF and decrease MVD in tumor to make tumor ischemia and necrosis in order to inhibit tumor growth.
Objective To study the effects of Xiaoaiping(XAP) with different concentrations on the tumor angiogenesis in mice and to discuss its possible mechanism in the inhibition of hepatic cellular cancer growth. Methods Seventy-five female KM mice whose weight is about(20±2)g were injected H22 liver tumor cells.They were randomly divided into five groups as following:D1(5-fluorouracil(5-Fu) was peritoneal injected,as a positive control),D2(NS was peritoneal injected,as a negative control),groups X1,X2 and X3 were peritoneal injected with XAP of 10 g/(kg·d),20 g/(kg·d) and 40 g/(kg ·d) respectively.The tumor′s volume was calculated to draw the tumor growth curve.The tissue of xenografted tumors was taken after 3 week′s treatment.The xenografted tumors were weighted its inhibitory rate was calculated.The pathological changes were observed by light microscope.The microvessel density(MVD) marked by CD34 in the xenografted tumor tissue was detected by immunohistochemisty.The concentration of serum VEGF was measured by ELISA. Results The inhibition rate in X3 and D1 group was similar(P>0.05).The doses of serum VEGF in X1,X2 and X3 group were all lower than that in D2 group(P<0.05),especially in X3group.To detect MVD,the conditions were the same as the dose of serum VEGF. Conclusion Different doses of XAP can inhibit tumor growth of H22 liver cancer in mice and the medium dose of XAP has the best inhibition.XAP may reduce VEGF and decrease MVD in tumor to make tumor ischemia and necrosis in order to inhibit tumor growth.
2012, 39(05): 502-505.
DOI: 10.3971/j.issn.1000-8578.2012.05.003
Abstract：
Objective To study the killing effect on liver cancers by mouse macrophages stimulated by Newcastle disease virus 7793 strain in vitro and the association of TRAIL. Methods The BALB/C mouse macrophages were harvested by using peritoneal lavage.And then the mouse macrophages were stimulate in vitro by NDV7793.The concentration of TNF-α and TRAIL was determined by ELISA after NDV stimulation.Then the macrophages of mice were coincubated and activated with Novikoff cells.The cytotoxic effect of macrophages on Novikoff cells was performed by Lactate Dehydrogenase(LDH) assay after NDV stimulation.Three experiment control groups were simultaneously set up as following:IFN-β positive control group,ultraviolet ray inactivated NDV(UV-NDV) control group as well as blank control group. Results Compared with three control groups in vitro,the macrophages stimulated with NDV 7793 had been activated,and the level of TNF-α and TRAIL in culture supernatant increased.The killing ability of macrophage to Novikoff cells after NDV stimulation had increased. Conclusion The NDV 7793 can activate the mouse macrophages in vitro.The killing effect on liver cancer cells of the mouse macrophages is enhanced by NDV stimulation.And it is possible that TRAIL and TNF-α involve in this may enhance the killing effect.
Objective To study the killing effect on liver cancers by mouse macrophages stimulated by Newcastle disease virus 7793 strain in vitro and the association of TRAIL. Methods The BALB/C mouse macrophages were harvested by using peritoneal lavage.And then the mouse macrophages were stimulate in vitro by NDV7793.The concentration of TNF-α and TRAIL was determined by ELISA after NDV stimulation.Then the macrophages of mice were coincubated and activated with Novikoff cells.The cytotoxic effect of macrophages on Novikoff cells was performed by Lactate Dehydrogenase(LDH) assay after NDV stimulation.Three experiment control groups were simultaneously set up as following:IFN-β positive control group,ultraviolet ray inactivated NDV(UV-NDV) control group as well as blank control group. Results Compared with three control groups in vitro,the macrophages stimulated with NDV 7793 had been activated,and the level of TNF-α and TRAIL in culture supernatant increased.The killing ability of macrophage to Novikoff cells after NDV stimulation had increased. Conclusion The NDV 7793 can activate the mouse macrophages in vitro.The killing effect on liver cancer cells of the mouse macrophages is enhanced by NDV stimulation.And it is possible that TRAIL and TNF-α involve in this may enhance the killing effect.
2012, 39(05): 506-510.
DOI: 10.3971/j.issn.1000-8578.2012.05.004
Abstract：
Objective To investigate the effect of small interfering RNA-mediated survivin knock-down on proliferation of human Cervical cancer Cell XB1702 and their sensitivity to Gefitinib. Methods The siRNA against survivin was constructed and transfected into XB1702 cells with LipofectamineTM 2000.The expression of survivin was detected by RT-PCR and Western blot.Flow cytometry was used to detect the cell cycle.Sensitivity to Geftinib after transfection were examined by MTT and clonogenic assay. Results In XB1702 cells,the protein and mRNA levels of survivin were decreased significantly after transfection,and reduction of proliferation was related to an increase in the fraction of G0/G1 phase.The sensitivity of XB1702 cells to Gefitinib increased significantly after transfection. Conclusion The survivin special siRNA silenced surviving,decreased XB1702 cells proliferation and enhanced their sensitivity to Gefitinib.
Objective To investigate the effect of small interfering RNA-mediated survivin knock-down on proliferation of human Cervical cancer Cell XB1702 and their sensitivity to Gefitinib. Methods The siRNA against survivin was constructed and transfected into XB1702 cells with LipofectamineTM 2000.The expression of survivin was detected by RT-PCR and Western blot.Flow cytometry was used to detect the cell cycle.Sensitivity to Geftinib after transfection were examined by MTT and clonogenic assay. Results In XB1702 cells,the protein and mRNA levels of survivin were decreased significantly after transfection,and reduction of proliferation was related to an increase in the fraction of G0/G1 phase.The sensitivity of XB1702 cells to Gefitinib increased significantly after transfection. Conclusion The survivin special siRNA silenced surviving,decreased XB1702 cells proliferation and enhanced their sensitivity to Gefitinib.
2012, 39(05): 511-514.
DOI: 10.3971/j.issn.1000-8578.2012.05.005
Abstract：
Objective To study the expression and the signification of marrow TPO and its receptor c-MPL in acute myeloid leukemia relapse after chemotherapy. Methods The expression of TPO and c-MPL of newly diagnosed acute myeloid leukemia patients before chemotherapy,complete remission after chemotherapy and its relapse was detected by using ELISA and flow cytometry,respectively. Results The expression of bone marrow TPO and c-MPL before chemotherapy was （162.21±39.92）ng/ml and （6.04±4.072）%，it was lower than the （213.19±48.34）ng/ml,（11.23±5.225）% of relapse group siglificantly.The expression of bone marrow TPO and c-MPL before chemotherapy and relapse group were significantly higher than （122.14± 25.75）ng/ml,（2.84±0.724）% of control group.The expression of bone marrow TPO and c-MPL of remission group was （115.82±22.32）ng/ml and （2.81±1.306）%.The expression of marrow TPO and c-MPL between chemotherapied AML patients and normal group was no significant difference(P>0.05).The expression of bone marrow TPO was positively correlated with c-MPL. Conclusion （1）The high expression of bone marrow TPO and its receptor c-MPL may be associated with the relapse of acute myeloid leukemia after treatment.（2） The expression of bone marrow TPO and c-MPL was significantly higher in relapse group than before chemotherapy.It suggested that TPO/c-MPL signaling pathway may play an important role in acute myeloid leukemia relapse and refractory after treatment.
Objective To study the expression and the signification of marrow TPO and its receptor c-MPL in acute myeloid leukemia relapse after chemotherapy. Methods The expression of TPO and c-MPL of newly diagnosed acute myeloid leukemia patients before chemotherapy,complete remission after chemotherapy and its relapse was detected by using ELISA and flow cytometry,respectively. Results The expression of bone marrow TPO and c-MPL before chemotherapy was （162.21±39.92）ng/ml and （6.04±4.072）%，it was lower than the （213.19±48.34）ng/ml,（11.23±5.225）% of relapse group siglificantly.The expression of bone marrow TPO and c-MPL before chemotherapy and relapse group were significantly higher than （122.14± 25.75）ng/ml,（2.84±0.724）% of control group.The expression of bone marrow TPO and c-MPL of remission group was （115.82±22.32）ng/ml and （2.81±1.306）%.The expression of marrow TPO and c-MPL between chemotherapied AML patients and normal group was no significant difference(P>0.05).The expression of bone marrow TPO was positively correlated with c-MPL. Conclusion （1）The high expression of bone marrow TPO and its receptor c-MPL may be associated with the relapse of acute myeloid leukemia after treatment.（2） The expression of bone marrow TPO and c-MPL was significantly higher in relapse group than before chemotherapy.It suggested that TPO/c-MPL signaling pathway may play an important role in acute myeloid leukemia relapse and refractory after treatment.
2012, 39(05): 515-518.
DOI: 10.3971/j.issn.1000-8578.2012.05.006
Abstract：
Objective To investigate the relationship between epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI) treatment effects and serum tumor markers in lung adenocarcinoma. Methods Forty-eight patients with advanced lung adenocarcinoma were treated with EGFR-TKI.The clinical features,survival time and the level of serum tumor markers for the patients before and after treatment were retrospectively analyzed. Results After EGFR-TKI treatment,the RR was 58.3% and DCR was 65.6% in lung adenocarcinoma;and median survival time was 13.2 months.Responses correlated significantly with smoking history and level of CEA or CA19-9 in serum(P<0.05).Patients with higher level of serum CEA and CA19-9 had higher disease control rate and longer survival(P<0.05). Conclusion Serum CA19-9 or CEA level can predict the response of EGFR-TKI to lung adenocarcinoma.
Objective To investigate the relationship between epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI) treatment effects and serum tumor markers in lung adenocarcinoma. Methods Forty-eight patients with advanced lung adenocarcinoma were treated with EGFR-TKI.The clinical features,survival time and the level of serum tumor markers for the patients before and after treatment were retrospectively analyzed. Results After EGFR-TKI treatment,the RR was 58.3% and DCR was 65.6% in lung adenocarcinoma;and median survival time was 13.2 months.Responses correlated significantly with smoking history and level of CEA or CA19-9 in serum(P<0.05).Patients with higher level of serum CEA and CA19-9 had higher disease control rate and longer survival(P<0.05). Conclusion Serum CA19-9 or CEA level can predict the response of EGFR-TKI to lung adenocarcinoma.
2012, 39(05): 519-525.
DOI: 10.3971/j.issn.1000-8578.2012.05.007
Abstract：
Objective To systematically evaluate the safety and feasibility of radical trachelectomy in patients with early-stage cervical cancer who desired to maintain fertility. Methods Electronic databases including Pubmed，EMBASE，Cochrane Library and Chinese full-text database were systematically searched for all relevant published studies on radical trachelectomy(RT)and(or) radical hysterectomy(RH) in patients with early stage of cervical cancer.Meta-analysis was performed on controlled trials of RT and RH,and homogeneous merger analysis was only performed on non-controlled of RT.The primary outcome measures were survival rate and pregnancy rate. Results A total of four controlled trials of RT and RH were included in this meta-analysis and 17 non-controlled trials of RT also met the inclusion criteria.No significant differences were found.The total pregnancy rate was 47% after radical trachelectomy in patients with early-stage cervical cancer. Conclusion Radical trachelectomy appears safe when performed by sufficient surgery.It can satisfy the patients'needs to preserve the fertility.The conclusion is needed to be further assessed by rigorously designed,random and double-blind,controlled clinical trials.
Objective To systematically evaluate the safety and feasibility of radical trachelectomy in patients with early-stage cervical cancer who desired to maintain fertility. Methods Electronic databases including Pubmed，EMBASE，Cochrane Library and Chinese full-text database were systematically searched for all relevant published studies on radical trachelectomy(RT)and(or) radical hysterectomy(RH) in patients with early stage of cervical cancer.Meta-analysis was performed on controlled trials of RT and RH,and homogeneous merger analysis was only performed on non-controlled of RT.The primary outcome measures were survival rate and pregnancy rate. Results A total of four controlled trials of RT and RH were included in this meta-analysis and 17 non-controlled trials of RT also met the inclusion criteria.No significant differences were found.The total pregnancy rate was 47% after radical trachelectomy in patients with early-stage cervical cancer. Conclusion Radical trachelectomy appears safe when performed by sufficient surgery.It can satisfy the patients'needs to preserve the fertility.The conclusion is needed to be further assessed by rigorously designed,random and double-blind,controlled clinical trials.
2012, 39(05): 526-531.
DOI: 10.3971/j.issn.1000-8578.2012.05.008
Abstract：
Objective To evaluate the relationship between polymorphism of p73 4G14C-4A14T and susceptibility to colorectal cancer. Methods Studies from 2000—2010 were retrieved through Cochrane Library,Medline,Elsevier,Ovid,Highwire Press,PubMed，web of science，BIOSIS Preview，Journal Citation Reports，FMJS and EMMC database without language limitation to collect relevant papers.Odds ratio of p73 4G14C-4A14T genotype distributions in the group of patients with colorectal cancer and the group of healthy control was calculated.The Meta-analysis was applied with RevMan 5.0 software for Heterogeneity test and pooled OR calculation.Result Five case-control studies with 919 cases and 1234 controls were analyzed by fixed effects model(FEM) meta-analysis method.The pooled Odds Ratio of p734G14C-4A14T genotype GC/GC vs.GC/AT+AT/AT was 0.84(95%CI:0.71~1.00),Z statistics were 1.95,GC/GC vs.AT/AT was 0.50(95%CI:0.35~0.70),Z statistics were 4.00.AT/AT vs.GC/GC+GC/AT was 1.94 (95%CI:1.39~2.70)，Z statistics were 3.91. Conclusion We found that the variant AT/AT homozygote was associated with a significantly increase risk of colorectal cancer.There was enough evidence currently to prove that there was a relationship between p73 4G14C-4A14T genetic polymorphism and susceptibility to colorectal cancer.It was demonstrated that the genetic polymorphism of p73 4G14C-4A14T was an important risk factor for susceptibility to human colorectal cancer.
Objective To evaluate the relationship between polymorphism of p73 4G14C-4A14T and susceptibility to colorectal cancer. Methods Studies from 2000—2010 were retrieved through Cochrane Library,Medline,Elsevier,Ovid,Highwire Press,PubMed，web of science，BIOSIS Preview，Journal Citation Reports，FMJS and EMMC database without language limitation to collect relevant papers.Odds ratio of p73 4G14C-4A14T genotype distributions in the group of patients with colorectal cancer and the group of healthy control was calculated.The Meta-analysis was applied with RevMan 5.0 software for Heterogeneity test and pooled OR calculation.Result Five case-control studies with 919 cases and 1234 controls were analyzed by fixed effects model(FEM) meta-analysis method.The pooled Odds Ratio of p734G14C-4A14T genotype GC/GC vs.GC/AT+AT/AT was 0.84(95%CI:0.71~1.00),Z statistics were 1.95,GC/GC vs.AT/AT was 0.50(95%CI:0.35~0.70),Z statistics were 4.00.AT/AT vs.GC/GC+GC/AT was 1.94 (95%CI:1.39~2.70)，Z statistics were 3.91. Conclusion We found that the variant AT/AT homozygote was associated with a significantly increase risk of colorectal cancer.There was enough evidence currently to prove that there was a relationship between p73 4G14C-4A14T genetic polymorphism and susceptibility to colorectal cancer.It was demonstrated that the genetic polymorphism of p73 4G14C-4A14T was an important risk factor for susceptibility to human colorectal cancer.
2012, 39(05): 532-534.
DOI: 10.3971/j.issn.1000-8578.2012.05.009
Abstract：
Objective To study the expression of Gli1 protein,a component of Hedgehog/Gli1 signal pathway,and its relationship with angiogenesis in human breast cancer. Methods The expression of Gli1 protein was investigated by immunohistohistochemical method in 79 cases of human breast cancer,68 cases of normal tissues adjacent to cancer and 42 cases of breast fibroadenoma tissues.The microvascular density(MVD) was examined by using immunohistochemical CD34 staining assay. Results The Gli1 expression was higher in breast carcinoma than that in adjacent normal tissues and in breast fibroadenoma(P<0.05).Expression of Gli1 was significant higher in TNM stage Ⅲ than that in TNM stageⅠ～Ⅱ(P<0.05) and also was higher with axillary lymphnode metastasis than without lymphnode metastasis(P<0.05).Expressions of Gli1 were positively correlated with MVD(r=0.325，P<0.05). Conclusion Gli1 protein is activated and plays an important role in development of breast cancer.Promoting angiogenesis might be one of its mechanisms.
Objective To study the expression of Gli1 protein,a component of Hedgehog/Gli1 signal pathway,and its relationship with angiogenesis in human breast cancer. Methods The expression of Gli1 protein was investigated by immunohistohistochemical method in 79 cases of human breast cancer,68 cases of normal tissues adjacent to cancer and 42 cases of breast fibroadenoma tissues.The microvascular density(MVD) was examined by using immunohistochemical CD34 staining assay. Results The Gli1 expression was higher in breast carcinoma than that in adjacent normal tissues and in breast fibroadenoma(P<0.05).Expression of Gli1 was significant higher in TNM stage Ⅲ than that in TNM stageⅠ～Ⅱ(P<0.05) and also was higher with axillary lymphnode metastasis than without lymphnode metastasis(P<0.05).Expressions of Gli1 were positively correlated with MVD(r=0.325，P<0.05). Conclusion Gli1 protein is activated and plays an important role in development of breast cancer.Promoting angiogenesis might be one of its mechanisms.
2012, 39(05): 535-537.
DOI: 10.3971/j.issn.1000-8578.2012.05.010
Abstract：
Objective To investigate the clinical pathological characteristics,survival and prognostic factors of patients with triple negative breast cancer. Methods The clinical and pathological data of 560 patients,confirmed as breast cancer in Zhongnan hospital of Wuhan university and hubei cancer hospital from Jan 2004 to Dec 2009,were collected.We analyzed the clinical characteristics,recurrence rate and survival rate of the triple-negative breast cancer(TNBC) patients by comparing the characteristics between the two groups(TNBC and non-TNBC groups). Results Compared with non-TNBC group,the patients of TNBC group were usually young,associated with large masses,high histological grade and lymph node metastasis.There were significant differences in median survival time and the 5-year disease-free rate between TNBC group and non-TNBC group.Tumor mass,lymph node metastasis and menopause were important prognostic factors. Conclusion Triple negative breast cancer is one subtype of breast cancer with poor prognosis.
Objective To investigate the clinical pathological characteristics,survival and prognostic factors of patients with triple negative breast cancer. Methods The clinical and pathological data of 560 patients,confirmed as breast cancer in Zhongnan hospital of Wuhan university and hubei cancer hospital from Jan 2004 to Dec 2009,were collected.We analyzed the clinical characteristics,recurrence rate and survival rate of the triple-negative breast cancer(TNBC) patients by comparing the characteristics between the two groups(TNBC and non-TNBC groups). Results Compared with non-TNBC group,the patients of TNBC group were usually young,associated with large masses,high histological grade and lymph node metastasis.There were significant differences in median survival time and the 5-year disease-free rate between TNBC group and non-TNBC group.Tumor mass,lymph node metastasis and menopause were important prognostic factors. Conclusion Triple negative breast cancer is one subtype of breast cancer with poor prognosis.
2012, 39(05): 538-541.
DOI: 10.3971/j.issn.1000-8578.2012.05.011
Abstract：
Objective To investigate the relationships between the expression of HER3,HER2,ER and PR,and their effects on the clinical prognosis of human breast carcinoma. Methods The expressions of HER3,HER2,ER and PR were studied in 136 cases with breast carcinoma by using SP immunohistochemistry.The endpoint was overall survival(OS). Results HER2,HER3,ER and PR expression was detected in 136 cases with positive rates of 50%,41.18%,60.9% and 27.21%,respectively.The expression intensity of HER3 in breast carcinoma had no significant correlation with the age,lymph node metastasis,pathological types,TNM stage,chemotherapy of the patients(P>0.05).Compared with those patients with HER3-negitive profiles,the patients with HER3-positive profiles had significantly lower OS(P=0.015).The patients with HER3(+)HER2(+) expression profiles had significantly lower OS(P=0.047) than other groups.There is no interaction between HER3 and PR expression(P=0.214)，but HER3 have a inverse relationship with ER expression(P=0.001).Overexpression of HER2 and HER3 was highly correlated(P=0.000). Conclusion HER3 expression is an independent predictor of breast cancer.Patients with HER3-negative have a longer OS. Key words：
Objective To investigate the relationships between the expression of HER3,HER2,ER and PR,and their effects on the clinical prognosis of human breast carcinoma. Methods The expressions of HER3,HER2,ER and PR were studied in 136 cases with breast carcinoma by using SP immunohistochemistry.The endpoint was overall survival(OS). Results HER2,HER3,ER and PR expression was detected in 136 cases with positive rates of 50%,41.18%,60.9% and 27.21%,respectively.The expression intensity of HER3 in breast carcinoma had no significant correlation with the age,lymph node metastasis,pathological types,TNM stage,chemotherapy of the patients(P>0.05).Compared with those patients with HER3-negitive profiles,the patients with HER3-positive profiles had significantly lower OS(P=0.015).The patients with HER3(+)HER2(+) expression profiles had significantly lower OS(P=0.047) than other groups.There is no interaction between HER3 and PR expression(P=0.214)，but HER3 have a inverse relationship with ER expression(P=0.001).Overexpression of HER2 and HER3 was highly correlated(P=0.000). Conclusion HER3 expression is an independent predictor of breast cancer.Patients with HER3-negative have a longer OS. Key words：
2012, 39(05): 542-546.
DOI: 10.3971/j.issn.1000-8578.2012.05.012
Abstract：
Objective To explore the effect of primary tumor location or combined with serum AFP on the prognosis and postoperative recurrence for patients with hepatocellular carcinoma. Methods A total of 256 patients,who underwent routine surgery and with long term follow-up from March 2005 to October 2009,were retrospectively analyzed in our study.Kaplan-Meier survival curve was used to analyze overall survival(OS) and time to recurrence(TTR) and Logrank test was used to calculate P values.Univariate and multivariate analysis of clinicopathological parameters in hepatocellular carcinoma(HCC) patients with postoperative recurrence were carried out by Cox-regression analysis. Results There was different in overall survival of HCC patients based on their primary tumor location(P=0.022).Furthermore,the group with left or double hepatic lobe tumor demonstrated a higher recurrence risk than that of patients in the group with right hepatic lobe tumor(P=0.002).In a stratified analysis,there was a significant difference in the postoperative recurrence rate under the subset of negative serum AFP(AFP<25 ng/ml)(P<0.001),whereas no difference in the subset of positive serum AFP(AFP≥25 ng/ml)(P=0.258).Univariate and multivariate analysis showed that metastasis,serum AFP and Portal vein tumor thrombus(PVTT) were 3 dependent recurrent risk factors,whereas primary tumor location was not. Conclusion Primary tumor location can be served as an important recurrent risk factor in HCC patients after hepatectomy.
Objective To explore the effect of primary tumor location or combined with serum AFP on the prognosis and postoperative recurrence for patients with hepatocellular carcinoma. Methods A total of 256 patients,who underwent routine surgery and with long term follow-up from March 2005 to October 2009,were retrospectively analyzed in our study.Kaplan-Meier survival curve was used to analyze overall survival(OS) and time to recurrence(TTR) and Logrank test was used to calculate P values.Univariate and multivariate analysis of clinicopathological parameters in hepatocellular carcinoma(HCC) patients with postoperative recurrence were carried out by Cox-regression analysis. Results There was different in overall survival of HCC patients based on their primary tumor location(P=0.022).Furthermore,the group with left or double hepatic lobe tumor demonstrated a higher recurrence risk than that of patients in the group with right hepatic lobe tumor(P=0.002).In a stratified analysis,there was a significant difference in the postoperative recurrence rate under the subset of negative serum AFP(AFP<25 ng/ml)(P<0.001),whereas no difference in the subset of positive serum AFP(AFP≥25 ng/ml)(P=0.258).Univariate and multivariate analysis showed that metastasis,serum AFP and Portal vein tumor thrombus(PVTT) were 3 dependent recurrent risk factors,whereas primary tumor location was not. Conclusion Primary tumor location can be served as an important recurrent risk factor in HCC patients after hepatectomy.
2012, 39(05): 547-550.
DOI: 10.3971/j.issn.1000-8578.2012.05.013
Abstract：
Objective To explore the relationship of CD34,8-OHdG and Ki67 expression with clinical characteristics and post-operational survival hepatitis B virus related hepatocellular carcinoma. Methods Follow-up was performed every three months for 5 years.The expression level of 8-OHdG,CD34 and Ki67 were quantified by immunohistochemical staining. Results The frequency of positive staining for 8-OHdG,CD34 and Ki67 in HBV-HCC tissues was 100%,100% and 96.74%,respectively.Furthermore,the expression level of CD34 was higher in HBV-HCC tissue than that in normal tissue.The expression of CD34,8-OHdG and Ki67 showed a positive correlation each other.Multivariate Cox regression analysis indicated that CD34,8-OHdG and Ki67 were the independent predictors of post-operational survival for HBV-HCC patients. Conclusion CD34,8-OHdG and Ki67 is independent predictors for the prognosis of HCC.
Objective To explore the relationship of CD34,8-OHdG and Ki67 expression with clinical characteristics and post-operational survival hepatitis B virus related hepatocellular carcinoma. Methods Follow-up was performed every three months for 5 years.The expression level of 8-OHdG,CD34 and Ki67 were quantified by immunohistochemical staining. Results The frequency of positive staining for 8-OHdG,CD34 and Ki67 in HBV-HCC tissues was 100%,100% and 96.74%,respectively.Furthermore,the expression level of CD34 was higher in HBV-HCC tissue than that in normal tissue.The expression of CD34,8-OHdG and Ki67 showed a positive correlation each other.Multivariate Cox regression analysis indicated that CD34,8-OHdG and Ki67 were the independent predictors of post-operational survival for HBV-HCC patients. Conclusion CD34,8-OHdG and Ki67 is independent predictors for the prognosis of HCC.
2012, 39(05): 551-554.
DOI: 10.3971/j.issn.1000-8578.2012.05.014
Abstract：
Objective To research the expressions of Snail and N-cadherin in osteosarcoma and to explore their roles in the carcinogenesis and progression of osteosarcoma. Methods Immunohistochemical assay(SP method) was used to detect the expressions of Snail and N-cadherin in 38 cases osteosarcoma tissues and 10 cases osteochondroma tissues. Results (1) Expression of Snail and N-cadherin in osteosarcoma was higher than that in osteochondroma,the comparative differences were significant(χ2=27.375，P<0.01,χ2 =21.849，P<0.01).(2) Expression of Snail in osteosarcoma were concerned with soft tissue involvement,Ennecking stage,and lung metastases;and the comparative differences were significant (P<0.05). However,the expression of Snail in osteosarcoma were irrelevant with gender,age and mass site (P>0.05).The expression of N- cadherin was positively correlated with that of Snail(r=0.421，P<0.05).(3) The survival rate of the patients with positive express of Snail and N-cadherin was significant lower than those with negative express of snail and N-cadherin protein in osteosarcoma;and the comparative differences were significant(P<0.05). Conclusion The overexpression of Snail and N-cadherin in osteosarcoma means that they play synergetic roles in the process and metastases of osteosarcoma and poor prognosis.
Objective To research the expressions of Snail and N-cadherin in osteosarcoma and to explore their roles in the carcinogenesis and progression of osteosarcoma. Methods Immunohistochemical assay(SP method) was used to detect the expressions of Snail and N-cadherin in 38 cases osteosarcoma tissues and 10 cases osteochondroma tissues. Results (1) Expression of Snail and N-cadherin in osteosarcoma was higher than that in osteochondroma,the comparative differences were significant(χ2=27.375，P<0.01,χ2 =21.849，P<0.01).(2) Expression of Snail in osteosarcoma were concerned with soft tissue involvement,Ennecking stage,and lung metastases;and the comparative differences were significant (P<0.05). However,the expression of Snail in osteosarcoma were irrelevant with gender,age and mass site (P>0.05).The expression of N- cadherin was positively correlated with that of Snail(r=0.421，P<0.05).(3) The survival rate of the patients with positive express of Snail and N-cadherin was significant lower than those with negative express of snail and N-cadherin protein in osteosarcoma;and the comparative differences were significant(P<0.05). Conclusion The overexpression of Snail and N-cadherin in osteosarcoma means that they play synergetic roles in the process and metastases of osteosarcoma and poor prognosis.
2012, 39(05): 555-557.
DOI: 10.3971/j.issn.1000-8578.2012.05.015
Abstract：
Objective To look for the methods of diagnosis and differential diagnosis of pulmonary sclerosing hemangioma (PSH). Methods The clinicopathological immunohistonchemical and frozen section diagnosis features were reviewed in 20 cases of PSH. Results In 20 cases,female,right lung,double lung,left lung was 18,11,1 and 8,respectively. The average age is 55 and ranged from 32 to 68 years.Nineteen cases were isolated lung nodules,and 1 case was double lung nodules.Based on CT results,17 cases were diagnosed as lung cancer,2 as esophageal cancer and pulmonary metastasis,and 1 as pulmonary hamartoma. Based on the results from 10 intraoperative frozen section,2 cases were diagnosed as carcinoid tumor,2 bronchioloalveolar carcinoma,1 descriptive PSH,and 5 inflammatory pseudo tumor.11 cases showed a mixture of the following four histological patterns:solid,papillary,sclerotic and hemangioma-like.There were two types cells of tumor cells,cuboidal cells covered the papillary surface,and lined fissure-cavity in the solid and vascular areas,and polygonal cells with consistent size and morphology in the solid areas and papillary axis.Immunohistochemical staining revealed that the polygonal cells expressed EMA,TTF-1,Vimentin,but didn't expreesed AE1/AE3.The surface of the cubical cells expressed AE1/AE3 and TTF-1,but didn't express Vimentin. Conclusion The PSH is particularly prevalent in isolation with lung nodules in the middle-aged and old women. It was usually misdiagnosed as lung cancer by CT.It was also misdiag nosed by frozen section assay.Although tle frozen section assay could provide higher accuracy in properties judgment,most PSH were hard diagnosed exactly.Postoperative histological section with immunohistochemical inspection is an effective method among the differential diagnosis ways.
Objective To look for the methods of diagnosis and differential diagnosis of pulmonary sclerosing hemangioma (PSH). Methods The clinicopathological immunohistonchemical and frozen section diagnosis features were reviewed in 20 cases of PSH. Results In 20 cases,female,right lung,double lung,left lung was 18,11,1 and 8,respectively. The average age is 55 and ranged from 32 to 68 years.Nineteen cases were isolated lung nodules,and 1 case was double lung nodules.Based on CT results,17 cases were diagnosed as lung cancer,2 as esophageal cancer and pulmonary metastasis,and 1 as pulmonary hamartoma. Based on the results from 10 intraoperative frozen section,2 cases were diagnosed as carcinoid tumor,2 bronchioloalveolar carcinoma,1 descriptive PSH,and 5 inflammatory pseudo tumor.11 cases showed a mixture of the following four histological patterns:solid,papillary,sclerotic and hemangioma-like.There were two types cells of tumor cells,cuboidal cells covered the papillary surface,and lined fissure-cavity in the solid and vascular areas,and polygonal cells with consistent size and morphology in the solid areas and papillary axis.Immunohistochemical staining revealed that the polygonal cells expressed EMA,TTF-1,Vimentin,but didn't expreesed AE1/AE3.The surface of the cubical cells expressed AE1/AE3 and TTF-1,but didn't express Vimentin. Conclusion The PSH is particularly prevalent in isolation with lung nodules in the middle-aged and old women. It was usually misdiagnosed as lung cancer by CT.It was also misdiag nosed by frozen section assay.Although tle frozen section assay could provide higher accuracy in properties judgment,most PSH were hard diagnosed exactly.Postoperative histological section with immunohistochemical inspection is an effective method among the differential diagnosis ways.
2012, 39(05): 558-562.
DOI: 10.3971/j.issn.1000-8578.2012.05.016
Abstract：
Objective To explore the application of individuation skin flap in tongue reconstruction, which may make a patient's swallowing and language function recover soon, increase a patient's compliance to surgery, and reduce a patient's mental trauma to the most degree.MethodSixty-seven cases of patients with tongue squamous cell carcinoma were given radical surgery and adopted by implementing individuation skin flap shift and transplantation for tongue reconstruction.The results of postoperative recovery situation,the occurrence of complications,surgical compliance,and the possible mental trauma of the patients were judged and analyzed. Results Sixty-seven cases were healed up during I period of surface wound.Thrombosis occurred in 1 patient,while he was rescued successfully.The other patients had no complications.Survival rate of skip flap is 100%.Most of the recreated tongue's shapes were good.Swallowing function returned to normal,and language functional recovery was excellent or good.Patient's psychological situation was good. Conclusion The surgery of individuation skin flap in tongue reconstruction showed high success rate with low incidence of complications, satisfactory functional recovery,and high compliance and minimal trauma for patients.
Objective To explore the application of individuation skin flap in tongue reconstruction, which may make a patient's swallowing and language function recover soon, increase a patient's compliance to surgery, and reduce a patient's mental trauma to the most degree.MethodSixty-seven cases of patients with tongue squamous cell carcinoma were given radical surgery and adopted by implementing individuation skin flap shift and transplantation for tongue reconstruction.The results of postoperative recovery situation,the occurrence of complications,surgical compliance,and the possible mental trauma of the patients were judged and analyzed. Results Sixty-seven cases were healed up during I period of surface wound.Thrombosis occurred in 1 patient,while he was rescued successfully.The other patients had no complications.Survival rate of skip flap is 100%.Most of the recreated tongue's shapes were good.Swallowing function returned to normal,and language functional recovery was excellent or good.Patient's psychological situation was good. Conclusion The surgery of individuation skin flap in tongue reconstruction showed high success rate with low incidence of complications, satisfactory functional recovery,and high compliance and minimal trauma for patients.
2012, 39(05): 563-566.
DOI: 10.3971/j.issn.1000-8578.2012.05.017
Abstract：
Objective To explore the early diagnosis,reoperation and the clinical effect of the cervical lymphatic metastatic recurrence in the esophagectomy. Methods Seventy-six cases with lymphatic metastasis recurrence were retrospectively analyzed in the post 15 years in our chest surgery.They were monitored by the cervical ultrasound and were diagnosed in time.All the patients with esophagectomy were reoperated by modified neck dissection.The perioperative situation and living conditions of these patients were observed. Results All the 76 cases were tolerably able to the modified neck dissection.The perioperative death didn't occur and the life quality were good.The postoperative pathologic outcome showed that the cervical lymph nodes metastatic recurrence in the postseophagectomy were mostly in the Ⅵ,Ⅴ and Ⅳ areas.But there were no specific transfer regularity.The postoperative complications were observed in 17.1% of respiratory infection.14.5% of recurrent laryngeal nerve damage,and 3.9% of chylous fistula.After the reoperation,1-,3- and 5-year survival rates was 76.3%(58/76),35.5%(27/76),27.6%(21/76),respectively. Conclusion The timely follow up to the patients with postesophagectomy is very necessary.The neck ultrasound examination and fine-needle biopsy play an important roles in discovering the cervical lymph nodes metastatic recurrence.Taking reoperation can obtain better clinical effect.The living quality could be improved and the survival term could be prolonged for the only cervical lymphatic metastatic recurrence with postesophagectomy.There is superior to the general radiotherapy.Reoperation is one of the main choices for the patients in cervical lymphatic relapse with postesophagectomy which is valuable to be recommended in clinic.
Objective To explore the early diagnosis,reoperation and the clinical effect of the cervical lymphatic metastatic recurrence in the esophagectomy. Methods Seventy-six cases with lymphatic metastasis recurrence were retrospectively analyzed in the post 15 years in our chest surgery.They were monitored by the cervical ultrasound and were diagnosed in time.All the patients with esophagectomy were reoperated by modified neck dissection.The perioperative situation and living conditions of these patients were observed. Results All the 76 cases were tolerably able to the modified neck dissection.The perioperative death didn't occur and the life quality were good.The postoperative pathologic outcome showed that the cervical lymph nodes metastatic recurrence in the postseophagectomy were mostly in the Ⅵ,Ⅴ and Ⅳ areas.But there were no specific transfer regularity.The postoperative complications were observed in 17.1% of respiratory infection.14.5% of recurrent laryngeal nerve damage,and 3.9% of chylous fistula.After the reoperation,1-,3- and 5-year survival rates was 76.3%(58/76),35.5%(27/76),27.6%(21/76),respectively. Conclusion The timely follow up to the patients with postesophagectomy is very necessary.The neck ultrasound examination and fine-needle biopsy play an important roles in discovering the cervical lymph nodes metastatic recurrence.Taking reoperation can obtain better clinical effect.The living quality could be improved and the survival term could be prolonged for the only cervical lymphatic metastatic recurrence with postesophagectomy.There is superior to the general radiotherapy.Reoperation is one of the main choices for the patients in cervical lymphatic relapse with postesophagectomy which is valuable to be recommended in clinic.
2012, 39(05): 567-569.
DOI: 10.3971/j.issn.1000-8578.2012.05.018
Abstract：
Objective To investigate the effects of Jinmi decoction on the serum vascular endothelial growth factor(VEGF) in non-small cell lung cancer and its anti-tumor mechanism. Methods Sixty cases of non-small cell lung cancer were randomly divided into combination therapy group(Jinmi decoction + chemotherapy) and chemotherapy group.The serum VEGF concentration was detected by ELISA in the two groups and in 20 normal controls. Results The serum VEGF concentration in non-small cell lung cancer was(389.45± 80.23)pg/ml,higher than that in normal controls(P<0.01).The concentration of serum VEGF after therapy in combination therapy group was significantly lower than that in chemotherapy group(231.52 ±83.91)pg/ml vs..(295.17±76.43)pg/ml,(P<0.05). Conclusion Jinmi decoction could obviously reduce the concentration of serum VEGF and inhibit tumor angiogenesis in non-small cell lung cancer.
Objective To investigate the effects of Jinmi decoction on the serum vascular endothelial growth factor(VEGF) in non-small cell lung cancer and its anti-tumor mechanism. Methods Sixty cases of non-small cell lung cancer were randomly divided into combination therapy group(Jinmi decoction + chemotherapy) and chemotherapy group.The serum VEGF concentration was detected by ELISA in the two groups and in 20 normal controls. Results The serum VEGF concentration in non-small cell lung cancer was(389.45± 80.23)pg/ml,higher than that in normal controls(P<0.01).The concentration of serum VEGF after therapy in combination therapy group was significantly lower than that in chemotherapy group(231.52 ±83.91)pg/ml vs..(295.17±76.43)pg/ml,(P<0.05). Conclusion Jinmi decoction could obviously reduce the concentration of serum VEGF and inhibit tumor angiogenesis in non-small cell lung cancer.
2012, 39(05): 570-572.
DOI: 10.3971/j.issn.1000-8578.2012.05.019
Abstract：
Objective To assess the influencing factors,treatment and prognosis of malignant tumor patients with venous thomboembolic events(VIE). Methods The clinical data, including the site of thrombosis,platelet,D-dimer and fibrinogen level,of 33 patients with malignant tumor and VTE were retrospectively analyzed. Results One case complicated with pulmonary embolism(PTE) died within 4 h.Among the other 32 cases with deep venous thrombosis(DVT),11 cases obtained complete response,8 partial response,and 14 progress proceed.D-dimer and fibrinogen increased when VTE occurred.The median survival time of patients with high level D-dimer was 80 d which was lower than those patients with low levels(420 d).They showed significant difference(P=0.026). Conclusion The main treatments of anti-VTE are anticoagulation and anti-assembly.D-dimer,Fibrinogen in the diagnosis and prognosis determination of the patients with VTE are important prognostic factors.
Objective To assess the influencing factors,treatment and prognosis of malignant tumor patients with venous thomboembolic events(VIE). Methods The clinical data, including the site of thrombosis,platelet,D-dimer and fibrinogen level,of 33 patients with malignant tumor and VTE were retrospectively analyzed. Results One case complicated with pulmonary embolism(PTE) died within 4 h.Among the other 32 cases with deep venous thrombosis(DVT),11 cases obtained complete response,8 partial response,and 14 progress proceed.D-dimer and fibrinogen increased when VTE occurred.The median survival time of patients with high level D-dimer was 80 d which was lower than those patients with low levels(420 d).They showed significant difference(P=0.026). Conclusion The main treatments of anti-VTE are anticoagulation and anti-assembly.D-dimer,Fibrinogen in the diagnosis and prognosis determination of the patients with VTE are important prognostic factors.
Evaluation of Two Administration Way on Curative Effects of Epirubicin in Treatment of Breast Cancer
2012, 39(05): 573-576.
DOI: 10.3971/j.issn.1000-8578.2012.05.020
Abstract：
Objective To evaluate the curative effects of Epirubicin(EPI) administered by means of micro-pumping and intravenous dripping in patients with breast cancer of the Ⅲ and the Ⅳ phases. Methods A total of 67 cases that were pathologically diagnosed as the Ⅲ- and Ⅳ-phase breast cancer were recruited in this study.Patients were randomly divided into two groups and received the same dosage of Epirubicin(70 mg/m2),which was administered by micro-pumping(MP group,n=33) and intravenous dripping(ID group,n=34),respectively.Chemotherapy repeated on a regular three-week cycle and observations of the curative effect were conducted in line with the WHO standard. Results Data from a total of 214 courses of chemotherapy were collected from the two groups of patients.All cases are assessable of their curative effects.In the MP group,10 cases of Ⅲ phase were specified as 2 cases of CR,6 PR,2 SD,but none of PD.The average remedial ratio(RR) reached 80.0%(8/10) and their disease control ratio(DCR) was 100.0%(10/10).Of the 23 cases of the Ⅳ phase(in which 7 cases was found with liver metastasis,15 with pulmonary metastasis,and 5 with brain metastasis),there was 2 cases of CR,13 PR,6 SD,and 2 PD.The average RR was 65.2%(15/23) and the DCR was 91.3%(21/23).In the ID group,9 cases of Ⅲ phase were specified as 1 case of CR,4 PR,3 SD,and 1 PD.The average RR was 55.6%(5/9) and the DCR was 88.9%(8/9).Of the 25 cases of the Ⅳ phase(in which 6 cases were found with liver metastasis,16 with pulmonary metastasis,and 4 with brain metastasis),there were 9 cases of PR,12 SD,4 PD,but none of CR.The average RR was 36.0%(9/25) and the DCR was 84.0%(21/25).Compared with the ID group,the curative effect of Epirubicin in the MP group turned out to be more prominent(χ2=5.509，P=0.027). Conclusion Administration of EPI by micro-pumping had better curative effect than by using intravenous dripping.Further study with large sample size should be carried out to validate our current observation.
Objective To evaluate the curative effects of Epirubicin(EPI) administered by means of micro-pumping and intravenous dripping in patients with breast cancer of the Ⅲ and the Ⅳ phases. Methods A total of 67 cases that were pathologically diagnosed as the Ⅲ- and Ⅳ-phase breast cancer were recruited in this study.Patients were randomly divided into two groups and received the same dosage of Epirubicin(70 mg/m2),which was administered by micro-pumping(MP group,n=33) and intravenous dripping(ID group,n=34),respectively.Chemotherapy repeated on a regular three-week cycle and observations of the curative effect were conducted in line with the WHO standard. Results Data from a total of 214 courses of chemotherapy were collected from the two groups of patients.All cases are assessable of their curative effects.In the MP group,10 cases of Ⅲ phase were specified as 2 cases of CR,6 PR,2 SD,but none of PD.The average remedial ratio(RR) reached 80.0%(8/10) and their disease control ratio(DCR) was 100.0%(10/10).Of the 23 cases of the Ⅳ phase(in which 7 cases was found with liver metastasis,15 with pulmonary metastasis,and 5 with brain metastasis),there was 2 cases of CR,13 PR,6 SD,and 2 PD.The average RR was 65.2%(15/23) and the DCR was 91.3%(21/23).In the ID group,9 cases of Ⅲ phase were specified as 1 case of CR,4 PR,3 SD,and 1 PD.The average RR was 55.6%(5/9) and the DCR was 88.9%(8/9).Of the 25 cases of the Ⅳ phase(in which 6 cases were found with liver metastasis,16 with pulmonary metastasis,and 4 with brain metastasis),there were 9 cases of PR,12 SD,4 PD,but none of CR.The average RR was 36.0%(9/25) and the DCR was 84.0%(21/25).Compared with the ID group,the curative effect of Epirubicin in the MP group turned out to be more prominent(χ2=5.509，P=0.027). Conclusion Administration of EPI by micro-pumping had better curative effect than by using intravenous dripping.Further study with large sample size should be carried out to validate our current observation.
2012, 39(05): 577-581.
DOI: 10.3971/j.issn.1000-8578.2012.05.021
Abstract：
Objective To compare the long term survival and side effects of the high-dose radiotherapy with the convention radiotherapy for locally advanced non-small cell lung cancer(NSCLC),and to analysis the prognostic factors. Methods From Jan,2000 to Jan,2004,75 patients underwent high-dose radiotherapy(HRT) and 83 patients received conventional radiotherapy(CRT) in non-small cell lung cancer were analyzed.The clinical evaluation of the patients in the recent 3 months after radiotherapy were carried out according RECIST criteria.The toxicity reaction to the patients were graded.SPSS11.5 software was used for statistical analysis. Results The two groups had the same variable.For all patients,the overall response rate(CR + PR) was 80.4%,and the 1-,3- and 5- year survival rates were 44.9 %,19.0% and 11.7%,respectively.There were no statistics significance between HRT group and CRT group.For all patients,the 1-,3- and 5-years local control rate was 76.8%,48.1% and 40.5%,respectively.There were statistics significance between HRT group and CRT group(χ2=3.80，P=0.041).The multivariate analysis showed that the maximum diameter of tumor,chemotherapy,treatment time and short-term curative effect were independent prognostic factors.Compared HRT group with CRT group,the treatment time was shorter(χ2=3.93，P=0.047),and the short-term curative effect was better(χ2=9.649，P=0.022).But,there were no statistics significance between the two groups in the rate of radiation injury. Conclusion The high-dose radiotherapy has a good long effective in the treatment of NSCLC.The treatment time was shorter and the side effects were not more.
Objective To compare the long term survival and side effects of the high-dose radiotherapy with the convention radiotherapy for locally advanced non-small cell lung cancer(NSCLC),and to analysis the prognostic factors. Methods From Jan,2000 to Jan,2004,75 patients underwent high-dose radiotherapy(HRT) and 83 patients received conventional radiotherapy(CRT) in non-small cell lung cancer were analyzed.The clinical evaluation of the patients in the recent 3 months after radiotherapy were carried out according RECIST criteria.The toxicity reaction to the patients were graded.SPSS11.5 software was used for statistical analysis. Results The two groups had the same variable.For all patients,the overall response rate(CR + PR) was 80.4%,and the 1-,3- and 5- year survival rates were 44.9 %,19.0% and 11.7%,respectively.There were no statistics significance between HRT group and CRT group.For all patients,the 1-,3- and 5-years local control rate was 76.8%,48.1% and 40.5%,respectively.There were statistics significance between HRT group and CRT group(χ2=3.80，P=0.041).The multivariate analysis showed that the maximum diameter of tumor,chemotherapy,treatment time and short-term curative effect were independent prognostic factors.Compared HRT group with CRT group,the treatment time was shorter(χ2=3.93，P=0.047),and the short-term curative effect was better(χ2=9.649，P=0.022).But,there were no statistics significance between the two groups in the rate of radiation injury. Conclusion The high-dose radiotherapy has a good long effective in the treatment of NSCLC.The treatment time was shorter and the side effects were not more.
2012, 39(05): 582-585.
DOI: 10.3971/j.issn.1000-8578.2012.05.022
Abstract：
Objective To explore the effects of service system for women and children health care on breast cancer screening. Methods A random cluster sampling was taken in the central districts of Wuhan, and 33 019 women within 35~59 years old were chosen to be study samples.Servise system for women and children health care was utilized to organize and manage breast cancer screening.Breast cancer screening method was carried out by combination of clinic breast examination with molybdenum target X-ray and high-frequency ultrasound examination.All lesions and prognosis were determined by pathology.Screening rates,review rates,breast cancer detection rate were used to evaluate the effect of service system for women and children health care on breast cancer screening. Results 30 478 of 33 019 women participated the breast cancer screening.The screen rate was 92.30%.The rates of screening molybdenum target and ultrasound examination was 92.47%.All patients who were diagnosed as breast cancer or suspected cancer through combination of clinical examination,molybdenum target X-ray and ultrasound underwent surgery.25 of women were diagnosed as breast cancer by pathology.The detection rate of the breast cancer screening was 82.03/100 000. Conclusion Depending on the breast cancer screening process organized by health service system for women and children care,the adherence to screening in women was increased and the medical technology was integrated better,which greatly enhanced the quality of breast cancer screening.
Objective To explore the effects of service system for women and children health care on breast cancer screening. Methods A random cluster sampling was taken in the central districts of Wuhan, and 33 019 women within 35~59 years old were chosen to be study samples.Servise system for women and children health care was utilized to organize and manage breast cancer screening.Breast cancer screening method was carried out by combination of clinic breast examination with molybdenum target X-ray and high-frequency ultrasound examination.All lesions and prognosis were determined by pathology.Screening rates,review rates,breast cancer detection rate were used to evaluate the effect of service system for women and children health care on breast cancer screening. Results 30 478 of 33 019 women participated the breast cancer screening.The screen rate was 92.30%.The rates of screening molybdenum target and ultrasound examination was 92.47%.All patients who were diagnosed as breast cancer or suspected cancer through combination of clinical examination,molybdenum target X-ray and ultrasound underwent surgery.25 of women were diagnosed as breast cancer by pathology.The detection rate of the breast cancer screening was 82.03/100 000. Conclusion Depending on the breast cancer screening process organized by health service system for women and children care,the adherence to screening in women was increased and the medical technology was integrated better,which greatly enhanced the quality of breast cancer screening.
