2011 Vol. 38 No. 04
2011, 38(04): 365-368.
DOI: 10.3971/j.issn.1000-8578.2011.04.001
Abstract：
ObjectiveTo investigate expression of Soluble Interleutin-2 Receptor(sIL-2R), vascular endothelial growth factor(VEGF) and CA125 in nude mice bearing human ovarian carcinoma induced by adverse psychological stress. Methods All nude mice were randomly divided into 4 groups(each n=6)： normal control group(Ⅰ),simple stress group(Ⅱ),tumor group(Ⅲ),and tumor plus stress group(Ⅳ).Adverse psychological stress model and human ovarian carcinoma xenografts model were established in correlated groups.The growth of tumors was observed in nude mice.The weight of subcutaneous tumor was measured，the concentrations of sIL-2R,VEGF and CA125 in serum were detected by enzyme-linked immunoabsorbent assay (ELISA). Results Compared with tumor group, human ovarian carcinoma xenografts growed faster in tumor plus stress group,the tumor growth rate was 66.33%（P<0.05）.The level of sIL-2R,VEGF in simple tress group was significantly higher than those in normal control group（P<0.05）.Expression of CA125 level in simple stress group was not significant difference with that innormal control group（P>0.05）.Tumor group and normal control group, tumor plus stress group and normal control group were significantly different compared with correlated group(P<0.05),the concentrations of sIL-2R,VEGF and CA125 in serum were significantly higher than that in correlated control group（P<0.05）. Conclusion Adverse psychological stress can suppress the immune function of tumor-bearing nude mice, and result in rapid growth of tumors.
ObjectiveTo investigate expression of Soluble Interleutin-2 Receptor(sIL-2R), vascular endothelial growth factor(VEGF) and CA125 in nude mice bearing human ovarian carcinoma induced by adverse psychological stress. Methods All nude mice were randomly divided into 4 groups(each n=6)： normal control group(Ⅰ),simple stress group(Ⅱ),tumor group(Ⅲ),and tumor plus stress group(Ⅳ).Adverse psychological stress model and human ovarian carcinoma xenografts model were established in correlated groups.The growth of tumors was observed in nude mice.The weight of subcutaneous tumor was measured，the concentrations of sIL-2R,VEGF and CA125 in serum were detected by enzyme-linked immunoabsorbent assay (ELISA). Results Compared with tumor group, human ovarian carcinoma xenografts growed faster in tumor plus stress group,the tumor growth rate was 66.33%（P<0.05）.The level of sIL-2R,VEGF in simple tress group was significantly higher than those in normal control group（P<0.05）.Expression of CA125 level in simple stress group was not significant difference with that innormal control group（P>0.05）.Tumor group and normal control group, tumor plus stress group and normal control group were significantly different compared with correlated group(P<0.05),the concentrations of sIL-2R,VEGF and CA125 in serum were significantly higher than that in correlated control group（P<0.05）. Conclusion Adverse psychological stress can suppress the immune function of tumor-bearing nude mice, and result in rapid growth of tumors.
2011, 38(04): 369-372.
DOI: 10.3971/j.issn.1000-8578.2011.04.002
Abstract：
ObjectiveTo explore the inhibitory effect of arsenic trioxide(ATO, As2O3) on the tumor growth of cervical cancer cell line HeLa subcutaneously implanted in nude mice and its mechanism. Methods Human cervical cancer xenografted model was established in nude mice.The tumor-bearing nude mice were randomly divided into the experimental groups： ATO low dose group ［2mg/（kg·d)］,ATO high dose group ［5mg/（kg·d)］,DDP positive control group ［3mg/（kg·d)］,saline negative control group(0.9%NaCl 0.2ml/d).The drugs were administered intraperitoneally for 10 consecutive days.To observe the tumor inhibition rate and effects of drugs.Ultramicrostructure feature of tumor was observed under electron microscope.Immunohistochemistry was used to measure the expression of p-P38 and Caspase-3. Results Inhibited tumor volume of ATO low and high dose groups and DDP positive control group was 22.95%,54.86% and 54.48%,respectively.The inhibited effect of ATO 5mg/kg/d group was similar with DDP 3mg/kg/d group, but the toxic effect of DDP was higher than ATO.The expression of p-P38 and Caspase-3 was higher than negative control group (P<0.05). Conclusion ATO can inhibit the growth of cervical cancer cells in vivo through enhancing apoptosis of tumor cells.
ObjectiveTo explore the inhibitory effect of arsenic trioxide(ATO, As2O3) on the tumor growth of cervical cancer cell line HeLa subcutaneously implanted in nude mice and its mechanism. Methods Human cervical cancer xenografted model was established in nude mice.The tumor-bearing nude mice were randomly divided into the experimental groups： ATO low dose group ［2mg/（kg·d)］,ATO high dose group ［5mg/（kg·d)］,DDP positive control group ［3mg/（kg·d)］,saline negative control group(0.9%NaCl 0.2ml/d).The drugs were administered intraperitoneally for 10 consecutive days.To observe the tumor inhibition rate and effects of drugs.Ultramicrostructure feature of tumor was observed under electron microscope.Immunohistochemistry was used to measure the expression of p-P38 and Caspase-3. Results Inhibited tumor volume of ATO low and high dose groups and DDP positive control group was 22.95%,54.86% and 54.48%,respectively.The inhibited effect of ATO 5mg/kg/d group was similar with DDP 3mg/kg/d group, but the toxic effect of DDP was higher than ATO.The expression of p-P38 and Caspase-3 was higher than negative control group (P<0.05). Conclusion ATO can inhibit the growth of cervical cancer cells in vivo through enhancing apoptosis of tumor cells.
2011, 38(04): 373-376.
DOI: 10.3971/j.issn.1000-8578.2011.04.003
Abstract：
ObjectiveTo explore the effects of Cetuximab combined with radiation on radiosensitivity, apoptosis and cell cycle progression of nasopharyngeal carcinoma (NPC). MethodsHuman NPC cell line CNE2 was treated with cetuximab, radiation or their combination.Using clonogenic survival, radiosensitivity was investigated.The Sigmaplot 10.0 software was applied for fitting the cell survival curve by single-hit multi-target model and linear-quadratic model. Apoptosis and cell cycle were measured by flow-cytometer. Results The radiosensitivity of CNE2 was enhanced by cetuximab and the sensitization enhancement ratio (SER) was 1.157.Radiation plus cetuximab significantly enhanced the radiation-induced apoptotic rates in CNE2 cell (P<0.05).By flow-cytometric analyses, G2/M arrest was induced in the NPC cells exposed to cetuximab alone.Furthermore, combined treatment resulted in synergistic effect on G2/M arrest in NPC cells. Conclusion Radiosensitivity of NPC cell lines was enhanced after exposure to cetuximab through promoting radiation-induced apoptosis and inducing cell cycle arrest.
ObjectiveTo explore the effects of Cetuximab combined with radiation on radiosensitivity, apoptosis and cell cycle progression of nasopharyngeal carcinoma (NPC). MethodsHuman NPC cell line CNE2 was treated with cetuximab, radiation or their combination.Using clonogenic survival, radiosensitivity was investigated.The Sigmaplot 10.0 software was applied for fitting the cell survival curve by single-hit multi-target model and linear-quadratic model. Apoptosis and cell cycle were measured by flow-cytometer. Results The radiosensitivity of CNE2 was enhanced by cetuximab and the sensitization enhancement ratio (SER) was 1.157.Radiation plus cetuximab significantly enhanced the radiation-induced apoptotic rates in CNE2 cell (P<0.05).By flow-cytometric analyses, G2/M arrest was induced in the NPC cells exposed to cetuximab alone.Furthermore, combined treatment resulted in synergistic effect on G2/M arrest in NPC cells. Conclusion Radiosensitivity of NPC cell lines was enhanced after exposure to cetuximab through promoting radiation-induced apoptosis and inducing cell cycle arrest.
Effects of Hepatocyte Growth Factor on Proliferation and Migration of Human Colon Cancer Cells SW620
2011, 38(04): 377-379.
DOI: 10.3971/j.issn.1000-8578.2011.04.004
Abstract：
ObjectiveTo investigate the effects of Hepatocyte growth factor (HGF) on the proliferation and migration of SW620. Methods MTT was used to assess the effect of HGF on the proliferation of SW620.The effect of HGF on the migration of cells was calculated by restoration of basal membrane；the ultrastructure of SW620 after being treated with HGF was observed by TEM；the expression of MMP-2 was determined by PCR. Results HGF promoted both the proliferation and migration of SW620 in a dose-dependent manner.Microfilaments increased significantly after being treated with HGF.HGF up-regulated the expression level of MMP-2 mRNA of SW620 cells. Conclusion HGF can promote the proliferation and migration of colon cancer cell SW620.
ObjectiveTo investigate the effects of Hepatocyte growth factor (HGF) on the proliferation and migration of SW620. Methods MTT was used to assess the effect of HGF on the proliferation of SW620.The effect of HGF on the migration of cells was calculated by restoration of basal membrane；the ultrastructure of SW620 after being treated with HGF was observed by TEM；the expression of MMP-2 was determined by PCR. Results HGF promoted both the proliferation and migration of SW620 in a dose-dependent manner.Microfilaments increased significantly after being treated with HGF.HGF up-regulated the expression level of MMP-2 mRNA of SW620 cells. Conclusion HGF can promote the proliferation and migration of colon cancer cell SW620.
2011, 38(04): 380-383.
DOI: 10.3971/j.issn.1000-8578.2011.04.005
Abstract：
ObjectiveThe levels of EBV-LMP2 special CTLs, EBV-DNA and T cell subsets in peripheral blood of NPC patients are analyzed to summarize the change patterns and characteristics. Methods Serum samples were obtained from 35 NPC patients waiting for radiotherapy, 40 NPC patients treated with radiotherapy and 30 volunteers without NPC.ELISPOT assay was used to evaluate the levels of EBV-LMP2 special CTLs.RT-PCR and flow cytometry were employed to detect EBV-DNA loads and T cell subsets contents,respectively. Results The positive percentages of EBV-LMP2-specific CTLs were obviously different among three groups；the healthy individuals appeared of highest response, the next was post-treated NPC patients.The positive percentages of EBV-DNA in NPC patients were higher than that in normal controls.Besides, the positive percentage of pre-treated patients was higher than that of post-treated patients.The positive percentage had a negative correlation between EBV-LMP2 special immune response and EBV-DNA.The percentages of CD3+， CD4+ T cells and the CD4+/CD8+ ratios in the pre-treated NPC group decreased；the percentage of CD8+ T cells and the CD4+CD25+ Tr cell level increased. Conclusion The special activity of CTLs in pre-treated NPC patients was lowest and correlated with the EBV-DNA load.Enhancing the response of CTLs may improve the effect on preventing and curing the EBV associated tumors.EBV-DNA load could reflect the tumor load and effectiveness of therapy in some way.The CD3+ and CD4+ T cells in peripheral blood of NPC patients decreased obviously；the levels of CD8+ T cells and CD4+CD25+Tr cell increased.The detection of the T cell subsets would be helpful to the treatment and prognosis judgement of NPC patients.
ObjectiveThe levels of EBV-LMP2 special CTLs, EBV-DNA and T cell subsets in peripheral blood of NPC patients are analyzed to summarize the change patterns and characteristics. Methods Serum samples were obtained from 35 NPC patients waiting for radiotherapy, 40 NPC patients treated with radiotherapy and 30 volunteers without NPC.ELISPOT assay was used to evaluate the levels of EBV-LMP2 special CTLs.RT-PCR and flow cytometry were employed to detect EBV-DNA loads and T cell subsets contents,respectively. Results The positive percentages of EBV-LMP2-specific CTLs were obviously different among three groups；the healthy individuals appeared of highest response, the next was post-treated NPC patients.The positive percentages of EBV-DNA in NPC patients were higher than that in normal controls.Besides, the positive percentage of pre-treated patients was higher than that of post-treated patients.The positive percentage had a negative correlation between EBV-LMP2 special immune response and EBV-DNA.The percentages of CD3+， CD4+ T cells and the CD4+/CD8+ ratios in the pre-treated NPC group decreased；the percentage of CD8+ T cells and the CD4+CD25+ Tr cell level increased. Conclusion The special activity of CTLs in pre-treated NPC patients was lowest and correlated with the EBV-DNA load.Enhancing the response of CTLs may improve the effect on preventing and curing the EBV associated tumors.EBV-DNA load could reflect the tumor load and effectiveness of therapy in some way.The CD3+ and CD4+ T cells in peripheral blood of NPC patients decreased obviously；the levels of CD8+ T cells and CD4+CD25+Tr cell increased.The detection of the T cell subsets would be helpful to the treatment and prognosis judgement of NPC patients.
2011, 38(04): 384-388.
DOI: 10.3971/j.issn.1000-8578.2011.04.006
Abstract：
ObjectiveTo analyze the characteristic of serum proteins in nasopharyngeal carcinoma (NPC) patients and establish serum markers pattern for the diagnosis of NPC. Methods Surface enhanced laser desorption ionization time of flight mass spectormetry(SELDI-TOF-MS) technology was applied to analyze serum samples including 102 cases of NPC patients and118 cases of healthy controls.Biomarker Pattern Software (BPS) was used to detect the protein peaks significantly different between NPC patients and healthy controls, then a diagnostic pattern was established.This pattern was further valuated by a blind test.This technology was used to analyze the differential expression of serum proteins mass spectra in patients grouped by clinical classification and EB virus infection. Results Twenty-five significantly different protein peaks were found in serum samples between NPC patients and healthy controls including twelve up-regulated protein peaks and thirteen down-regulated protein peaks.Using BPS, a diagnostic pattern was established.A blind test generated a sensitivity of 97.56％, specificity of 88.89％ and accuracy of 92.63％ respectively.M/Z values of 10286Da,7569Da and 8149Da were significantly different between ascending type and descending type of NPC （P<0.05）；M/Z value of 9354Da and 4596Da were significantly different between EB negative and EB positive respectively（P<0.05）. Conclusion SELDI-TOF-MS technology can identify the significantly different protein peaks and establish a diagnostic pattern with high sensitivity and specificity.It will provide a highly accurate approach for the diagnosis of NPC，and SELDI could be used to discover some characteristic of serum proteins relates to different clinical features.
ObjectiveTo analyze the characteristic of serum proteins in nasopharyngeal carcinoma (NPC) patients and establish serum markers pattern for the diagnosis of NPC. Methods Surface enhanced laser desorption ionization time of flight mass spectormetry(SELDI-TOF-MS) technology was applied to analyze serum samples including 102 cases of NPC patients and118 cases of healthy controls.Biomarker Pattern Software (BPS) was used to detect the protein peaks significantly different between NPC patients and healthy controls, then a diagnostic pattern was established.This pattern was further valuated by a blind test.This technology was used to analyze the differential expression of serum proteins mass spectra in patients grouped by clinical classification and EB virus infection. Results Twenty-five significantly different protein peaks were found in serum samples between NPC patients and healthy controls including twelve up-regulated protein peaks and thirteen down-regulated protein peaks.Using BPS, a diagnostic pattern was established.A blind test generated a sensitivity of 97.56％, specificity of 88.89％ and accuracy of 92.63％ respectively.M/Z values of 10286Da,7569Da and 8149Da were significantly different between ascending type and descending type of NPC （P<0.05）；M/Z value of 9354Da and 4596Da were significantly different between EB negative and EB positive respectively（P<0.05）. Conclusion SELDI-TOF-MS technology can identify the significantly different protein peaks and establish a diagnostic pattern with high sensitivity and specificity.It will provide a highly accurate approach for the diagnosis of NPC，and SELDI could be used to discover some characteristic of serum proteins relates to different clinical features.
2011, 38(04): 389-393.
DOI: 10.3971/j.issn.1000-8578.2011.04.007
Abstract：
ObjectiveTo study the relationship between expression of Annexin A5 and the differentiation and metastasis of nasopharyngeal carcinoma (NPC）. Methods The expression of Annexin A5 in specimens of 65 of NPC, 18 of lymph node metastasizing NPC (LMNPC) and 20 of chronic rhinitis (CR) were detected by immunohistochemistry. The correlation between the expression of Annexin A5 and clinic factors was analyzed.The expression of Annexin A5 in NPC cell lines with differential differentiation degree and metastasis potency was detected by Western blot.Observe the change of invasive capability of NPC cells after the downregulation of Annexin A5 by siRNA. Results The positive ratio of Annexin A5 in NPC, LMNPC and CR were 84.6%(55/65), 94.4%(17/18) and 10.0%(2/20), respectively.There were significant difference in the expression of Annexin A5 between NPC and LMNPC, NPC and CR (P<0.05).There were correlation among the expression of Annexin A5 and histological type, regional meastasis, distant metastasis, clinic stage and recurrence (P<0.05).And was no relationship with ages and gender.There was relationship among expression of Annexin A5 and differentiation and metastasis of NPC.Downregulation of Annexin A5 can decrease the invasive capability of 5-8F cells. Conclusion There was relationship among the expression of Annexin A5 and the differentiation and metastasis of NPC, and could serve as a biomarker of differentiation, metastasis and prognosis in NPC.
ObjectiveTo study the relationship between expression of Annexin A5 and the differentiation and metastasis of nasopharyngeal carcinoma (NPC）. Methods The expression of Annexin A5 in specimens of 65 of NPC, 18 of lymph node metastasizing NPC (LMNPC) and 20 of chronic rhinitis (CR) were detected by immunohistochemistry. The correlation between the expression of Annexin A5 and clinic factors was analyzed.The expression of Annexin A5 in NPC cell lines with differential differentiation degree and metastasis potency was detected by Western blot.Observe the change of invasive capability of NPC cells after the downregulation of Annexin A5 by siRNA. Results The positive ratio of Annexin A5 in NPC, LMNPC and CR were 84.6%(55/65), 94.4%(17/18) and 10.0%(2/20), respectively.There were significant difference in the expression of Annexin A5 between NPC and LMNPC, NPC and CR (P<0.05).There were correlation among the expression of Annexin A5 and histological type, regional meastasis, distant metastasis, clinic stage and recurrence (P<0.05).And was no relationship with ages and gender.There was relationship among expression of Annexin A5 and differentiation and metastasis of NPC.Downregulation of Annexin A5 can decrease the invasive capability of 5-8F cells. Conclusion There was relationship among the expression of Annexin A5 and the differentiation and metastasis of NPC, and could serve as a biomarker of differentiation, metastasis and prognosis in NPC.
2011, 38(04): 394-398.
DOI: 10.3971/j.issn.1000-8578.2011.04.008
Abstract：
Objective Dentritic cells (DCs) from malignant pleural effusions of patients with lung cancer were isolated and induced.Effect of DCs on proliferation and cytotoxicity of tumor infiltration lymphocytes (TILs) from origin malignant pleural effusions was studied. Methods Pleural effusion mononuclear cells were obtained from 16 patients with lung cancer.Cells were separated by density gradient and magnetic cell sorting system.DCs were induced by interleukin-4 (IL-4), tumor necrosis factor-α(TNF-α), and granulocyte-macrophage colony stimulating.DCs were observed by optical microscope, electronic microscope and flow cytometry respectively.TILs from origin malignant pleural effusions were induced by IL-2 and phytohemagglutinin.The cancer cells were separated with HEA-125 magnetic cell sorting system.The ability of stimulating the proliferation of TILs was detected with 3H-thymidine.The anti-tumor effect of TILs was measured with MTT method. Results The mature DCs from malignant pleural effusions of patients with lung cancer could be induced.The typical morphology in DCs was observed with optical and electronic microscopes.DCs expressed high-level surface phenotypes, including HLA-ABC, HLA-DR, CD86, CD54 and CD83, CD1a.DCs can increase about 1.7 times proliferation of TILs.The cytotoxicity of TILs stimulated with DCs was increased from (31.80±14.05)% to (51.89±13.27)%. Conclusion The mature DCs can be induced from malignant pleural effusions from patients with lung cancer.The DCs can stimulate TILs from the origin malignant pleural effusion and increase its proliferation ability and cytotoxicity to cancer cells.
Objective Dentritic cells (DCs) from malignant pleural effusions of patients with lung cancer were isolated and induced.Effect of DCs on proliferation and cytotoxicity of tumor infiltration lymphocytes (TILs) from origin malignant pleural effusions was studied. Methods Pleural effusion mononuclear cells were obtained from 16 patients with lung cancer.Cells were separated by density gradient and magnetic cell sorting system.DCs were induced by interleukin-4 (IL-4), tumor necrosis factor-α(TNF-α), and granulocyte-macrophage colony stimulating.DCs were observed by optical microscope, electronic microscope and flow cytometry respectively.TILs from origin malignant pleural effusions were induced by IL-2 and phytohemagglutinin.The cancer cells were separated with HEA-125 magnetic cell sorting system.The ability of stimulating the proliferation of TILs was detected with 3H-thymidine.The anti-tumor effect of TILs was measured with MTT method. Results The mature DCs from malignant pleural effusions of patients with lung cancer could be induced.The typical morphology in DCs was observed with optical and electronic microscopes.DCs expressed high-level surface phenotypes, including HLA-ABC, HLA-DR, CD86, CD54 and CD83, CD1a.DCs can increase about 1.7 times proliferation of TILs.The cytotoxicity of TILs stimulated with DCs was increased from (31.80±14.05)% to (51.89±13.27)%. Conclusion The mature DCs can be induced from malignant pleural effusions from patients with lung cancer.The DCs can stimulate TILs from the origin malignant pleural effusion and increase its proliferation ability and cytotoxicity to cancer cells.
2011, 38(04): 399-403.
DOI: 10.3971/j.issn.1000-8578.2011.04.009
Abstract：
ObjectiveTo study the methylation status of DLC1 gene promoter regions and the expression level of DLC1 protein in the development of sporadic breast cancer (SBC), and to research the epigenetics of aberrant DLC1 gene in SBC. Methods Methylation specific polymerase chain reaction (MSP) was used to study the methylation status of DLC1 promoter regions in 42 samples of SBC, 14 cases of breast atypital ductal hyperplasia (BADH), 20 cases of breast adenosis (BA) and 5 samples of peripheral lymphocytes from healthy adult women.The expression of DLC1 protein was detected by immunohistochemistry staining (EnVision method). Results We found DLC1 promoter methylation rate was 38.1% in SBC tissues.In BADH tissues，(35.7%) showed aberrant methylation of DLC1 gene promoter.While in the tissues of BA and 5 cases healthy adult women's peripheral lymphocytes， no methylation of DLC1 gene was found.In 42 cases of SBC， 26 cases (61.91%) showed loss expression of DLC1 protein and 16 cases (38.10%) showed reduction expression.In 14 BADH tissues， 6 cases (42.86%) showed negative expression of DLC1 protein, 8 cases (57.14%) showed reduced expression.No reduced or loss expression of DLC1 protein was found in BA tissues.In the 16 cases of DLC1 methylated SBC tissues, 14 cases showed no expression of DLC1 protein, another 2 cases showed weakly express DLC1 protein.In the 5 cases of DLC1 methylated BADH tissues, 3 cases negatively express, 2 cases showed reduced expression of DLC1 protein.Statistical analysis showed the methylation rates of DLC1 gene in SBC and BADH tissues were higher than that of BA.The difference in the three groups had statistical significance (P<0.01).While the methylation rates of DLC1 gene between SBC and BADH tissues were no difference.The expression rates of DLC1 protein in SBC and BADH tissues were lower than BA.And the expression rate of DLC1 protein in SBC is significantly lower than that of BADH.The positive intensities of DLC1 protein of DLC1 unmethylated group is higher than that of DLC1 methylated group(P<0.01). Conclusion The promoter methylation of DLC1 gene is an early event in the development of SBC and may contribute to the reduction or loss expression of DLC1 protein.The promoter methylation of DLC1 gene may play an important biological role in breast carcinogenesis.
ObjectiveTo study the methylation status of DLC1 gene promoter regions and the expression level of DLC1 protein in the development of sporadic breast cancer (SBC), and to research the epigenetics of aberrant DLC1 gene in SBC. Methods Methylation specific polymerase chain reaction (MSP) was used to study the methylation status of DLC1 promoter regions in 42 samples of SBC, 14 cases of breast atypital ductal hyperplasia (BADH), 20 cases of breast adenosis (BA) and 5 samples of peripheral lymphocytes from healthy adult women.The expression of DLC1 protein was detected by immunohistochemistry staining (EnVision method). Results We found DLC1 promoter methylation rate was 38.1% in SBC tissues.In BADH tissues，(35.7%) showed aberrant methylation of DLC1 gene promoter.While in the tissues of BA and 5 cases healthy adult women's peripheral lymphocytes， no methylation of DLC1 gene was found.In 42 cases of SBC， 26 cases (61.91%) showed loss expression of DLC1 protein and 16 cases (38.10%) showed reduction expression.In 14 BADH tissues， 6 cases (42.86%) showed negative expression of DLC1 protein, 8 cases (57.14%) showed reduced expression.No reduced or loss expression of DLC1 protein was found in BA tissues.In the 16 cases of DLC1 methylated SBC tissues, 14 cases showed no expression of DLC1 protein, another 2 cases showed weakly express DLC1 protein.In the 5 cases of DLC1 methylated BADH tissues, 3 cases negatively express, 2 cases showed reduced expression of DLC1 protein.Statistical analysis showed the methylation rates of DLC1 gene in SBC and BADH tissues were higher than that of BA.The difference in the three groups had statistical significance (P<0.01).While the methylation rates of DLC1 gene between SBC and BADH tissues were no difference.The expression rates of DLC1 protein in SBC and BADH tissues were lower than BA.And the expression rate of DLC1 protein in SBC is significantly lower than that of BADH.The positive intensities of DLC1 protein of DLC1 unmethylated group is higher than that of DLC1 methylated group(P<0.01). Conclusion The promoter methylation of DLC1 gene is an early event in the development of SBC and may contribute to the reduction or loss expression of DLC1 protein.The promoter methylation of DLC1 gene may play an important biological role in breast carcinogenesis.
2011, 38(04): 404-407.
DOI: 10.3971/j.issn.1000-8578.2011.04.010
Abstract：
Abstract:ObjectiveTo study the efficacy and factors that affect peripheral blood stem cell （PBSC）mobilization of patients with breast cancer treated by paclitaxel plus recombinant human G-CSF(rhG-CSF). Methods Twenty-six patients with breast cancer were included in our study.PTX at the dose of 175 mg/m2 was continuously given for 24 hours.When the number of white blood cell reached 1.0×109/L，rhG-CSF was given until the end of leukopheresis.The mononuclear cells （MNC）and CD34+ cells collected by a continuous flow cell separator from peripheral blood were analyzed with FACS.Various factors affecting the yield of CD34+，including age, the lowest leucocyte counts after chemotherapy, the blood cell counts before leukopheresis, pathological stage, prior chemotherapy were analyzed. Results The leukopheresis was started on the median 10th day following mobilization chemotherapy.The mean values of MNC and CD34+cells were (7.89±1.45)×108/kg and (4.88±1.54)×106/kg,respectively.The age was related with CD34+ cells counts.The other factors didn't significantly affect the CD34+ cells counts （P>0.05）.No serious side effect was observed. Conclusion PTX combined with rhG-CSF was an effective and safe mobilization regimen for autologous peripheral blood stem progenitor cells of patients with breast cancer.The age affected the collected number of CD34+ cells.
Abstract:ObjectiveTo study the efficacy and factors that affect peripheral blood stem cell （PBSC）mobilization of patients with breast cancer treated by paclitaxel plus recombinant human G-CSF(rhG-CSF). Methods Twenty-six patients with breast cancer were included in our study.PTX at the dose of 175 mg/m2 was continuously given for 24 hours.When the number of white blood cell reached 1.0×109/L，rhG-CSF was given until the end of leukopheresis.The mononuclear cells （MNC）and CD34+ cells collected by a continuous flow cell separator from peripheral blood were analyzed with FACS.Various factors affecting the yield of CD34+，including age, the lowest leucocyte counts after chemotherapy, the blood cell counts before leukopheresis, pathological stage, prior chemotherapy were analyzed. Results The leukopheresis was started on the median 10th day following mobilization chemotherapy.The mean values of MNC and CD34+cells were (7.89±1.45)×108/kg and (4.88±1.54)×106/kg,respectively.The age was related with CD34+ cells counts.The other factors didn't significantly affect the CD34+ cells counts （P>0.05）.No serious side effect was observed. Conclusion PTX combined with rhG-CSF was an effective and safe mobilization regimen for autologous peripheral blood stem progenitor cells of patients with breast cancer.The age affected the collected number of CD34+ cells.
2011, 38(04): 408-410.
DOI: 10.3971/j.issn.1000-8578.2011.04.011
Abstract：
ObjectiveTo investigate the methylation status of RAR-β2 gene and its dignostical value in sporadic breast cancer. Methods Sixty-two sporadic breast cancer tissues,15 benign breast tissues and 10 normal breast tissues were collected and subjected to methylation-specific PCR(MSP) and real-time quantitive PCR. Results Methylated rates of RAR-β2 gene promoter were observed in 50%(31/62) of breast cancer tissues, and in 20%(3/15) in benign proliferative breast tissues,but not in normal breast tissues.The prevalence of RAR-β2 gene promoter methylation in malignant was significantly higher than those in benign and normal breast tissues.No association was observed between RAR-β2 gene promoter methylation and patient's clinical-biologic characteristics including age, menostasis， but association was observed between RAR-β2 gene promoter methylation and the patients of the lymph node metastasis and clinical typing. Conclusion Our results suggest that promoter hypermethylation of RAR-β2 gene may be involved in the carcinogenesis of sporadic breast cancer.There is the correlation between promoter hypermethylation of RAR-β2 gene and the patients of the lymph node metastasis and clinical typing.
ObjectiveTo investigate the methylation status of RAR-β2 gene and its dignostical value in sporadic breast cancer. Methods Sixty-two sporadic breast cancer tissues,15 benign breast tissues and 10 normal breast tissues were collected and subjected to methylation-specific PCR(MSP) and real-time quantitive PCR. Results Methylated rates of RAR-β2 gene promoter were observed in 50%(31/62) of breast cancer tissues, and in 20%(3/15) in benign proliferative breast tissues,but not in normal breast tissues.The prevalence of RAR-β2 gene promoter methylation in malignant was significantly higher than those in benign and normal breast tissues.No association was observed between RAR-β2 gene promoter methylation and patient's clinical-biologic characteristics including age, menostasis， but association was observed between RAR-β2 gene promoter methylation and the patients of the lymph node metastasis and clinical typing. Conclusion Our results suggest that promoter hypermethylation of RAR-β2 gene may be involved in the carcinogenesis of sporadic breast cancer.There is the correlation between promoter hypermethylation of RAR-β2 gene and the patients of the lymph node metastasis and clinical typing.
2011, 38(04): 411-415.
DOI: 10.3971/j.issn.1000-8578.2011.04.012
Abstract：
ObjectiveTo investigate the expression level of hepatocyte growth factor（HGF) and its receptor, the proto-oncogene c-Met, and vascular endothelial growth factor-C(VEGF-C) in cervical carcinoma. Methods The mRNA levels of HGF,c-Met and VEGF-C were detected by Real Time fluorescence Quantitative PCR(FQ RT-PCR) method in 43 cervical carcinoma tissues，30 cervical intraepithelial neoplasm Ⅲ（CINⅢ）tissues and 27 normal cervical,respectively. Results The positive expression of HGF mRNA,c-Met mRNA and VEGF-C mRNA increased remarkably from normal tissue to CINⅢ and then to cervical carcinoma (P<0.05)．In cervical carcinoma, the expression of these three genes was positive correlated with the clinical stage,tumor size,the invasion of deep muscular layer and the lymph node metastasis (P<0.05).These three genes were significant positive correlation (P<0.01). Conclusion Over expression of HGF, c-Met and VEGF-C together may participate in the invasion and metastasis of cervical carcinoma.To investigate them together would be facilitated to the treatment and prognosis of cervical carcinoma.
ObjectiveTo investigate the expression level of hepatocyte growth factor（HGF) and its receptor, the proto-oncogene c-Met, and vascular endothelial growth factor-C(VEGF-C) in cervical carcinoma. Methods The mRNA levels of HGF,c-Met and VEGF-C were detected by Real Time fluorescence Quantitative PCR(FQ RT-PCR) method in 43 cervical carcinoma tissues，30 cervical intraepithelial neoplasm Ⅲ（CINⅢ）tissues and 27 normal cervical,respectively. Results The positive expression of HGF mRNA,c-Met mRNA and VEGF-C mRNA increased remarkably from normal tissue to CINⅢ and then to cervical carcinoma (P<0.05)．In cervical carcinoma, the expression of these three genes was positive correlated with the clinical stage,tumor size,the invasion of deep muscular layer and the lymph node metastasis (P<0.05).These three genes were significant positive correlation (P<0.01). Conclusion Over expression of HGF, c-Met and VEGF-C together may participate in the invasion and metastasis of cervical carcinoma.To investigate them together would be facilitated to the treatment and prognosis of cervical carcinoma.
2011, 38(04): 416-419.
DOI: 10.3971/j.issn.1000-8578.2011.04.013
Abstract：
ObjectiveTo investigate the influence of two different prone positions with belly-board on target motions and their dose distributions in pelvic 3-Dimension intensity-modulated radiation radiotherapy of cervical cancer. Methods The motion ranges of GTV under two different prone positions with belly-board (The first prone position： the lower end of belly-board hole opening near the upper end of lumbosacral junction；the second prone position： the lower end of belly-board hole opening near the lower end of the sacroiliac joints) and the dose-volume histograms of GTV, CTV and PTVwere measured for all 10 cervical cancer patients received pelvic radiotherapy, and the relationship of them was compared. ResultsThe target motions under two different prone positions with belly-board are existed in the abdomen-back' directions (1~1.5cm),and have statistical significance (P<0.05),while there is no statistical significance in the head-feet' directions and left-right' directions(P>0.05)；But the dose-volume histogram changes of GTV, CTV and PTV under two different prone positions with belly-board are no Statistical significance(P>0.05). Conclusion The target motions under two different prone positions with belly-board are existed， but they have no influence on dose -volume histograms of themselves.
ObjectiveTo investigate the influence of two different prone positions with belly-board on target motions and their dose distributions in pelvic 3-Dimension intensity-modulated radiation radiotherapy of cervical cancer. Methods The motion ranges of GTV under two different prone positions with belly-board (The first prone position： the lower end of belly-board hole opening near the upper end of lumbosacral junction；the second prone position： the lower end of belly-board hole opening near the lower end of the sacroiliac joints) and the dose-volume histograms of GTV, CTV and PTVwere measured for all 10 cervical cancer patients received pelvic radiotherapy, and the relationship of them was compared. ResultsThe target motions under two different prone positions with belly-board are existed in the abdomen-back' directions (1~1.5cm),and have statistical significance (P<0.05),while there is no statistical significance in the head-feet' directions and left-right' directions(P>0.05)；But the dose-volume histogram changes of GTV, CTV and PTV under two different prone positions with belly-board are no Statistical significance(P>0.05). Conclusion The target motions under two different prone positions with belly-board are existed， but they have no influence on dose -volume histograms of themselves.
2011, 38(04): 420-422.
DOI: 10.3971/j.issn.1000-8578.2011.04.014
Abstract：
ObjectiveTo explore the circumstance of hTERC gene amplification in the differ cervix disease and the forecasting significance between hTERC gene amplification and developmental tendency of cervix. Methods Using fluorescence microscope to observe the crossing effection of centromeric probe for 3 chromosome and the probe of hTERC gene. The circumstance of hTERC gene amplification is detected in the differ cervix disease.Meanwhile,the cases which have hTERC gene amplification are followed up. Results Amplification of hTERC gene is enhanced along with the development of cervix disease which has statistical significance. Conclusion There is close correlation between the hTERC gene amplification and the development of cervix disease,Using FISH to detect hTERC gene amplification may be the dependable means to forecast the developmental tendency of cervix disease.
ObjectiveTo explore the circumstance of hTERC gene amplification in the differ cervix disease and the forecasting significance between hTERC gene amplification and developmental tendency of cervix. Methods Using fluorescence microscope to observe the crossing effection of centromeric probe for 3 chromosome and the probe of hTERC gene. The circumstance of hTERC gene amplification is detected in the differ cervix disease.Meanwhile,the cases which have hTERC gene amplification are followed up. Results Amplification of hTERC gene is enhanced along with the development of cervix disease which has statistical significance. Conclusion There is close correlation between the hTERC gene amplification and the development of cervix disease,Using FISH to detect hTERC gene amplification may be the dependable means to forecast the developmental tendency of cervix disease.
2011, 38(04): 423-426.
DOI: 10.3971/j.issn.1000-8578.2011.04.015
Abstract：
ObjectiveTo investigate the expression of S100P on RNA and protein levels in relation to clinicopathological features with gastric cancer patients. Methods Expression of S100P in primary gastric cancer (n=93) was analyzed using immunohistochemistry.Then, immunoblotting and RT-PCR were conducted on part of immunohistochemical-positively stained gastric cancer tissues and matched gastric mucosas. Results The immunohistochemical staining for S100P was predominantly nuclear with some cytoplasmic staining in normal gastric mucosa.As a result, 52.7% (49/93) of primary tumors and almost all the normal gastric mucosa were stained positively and S100P showed down-regulated expression in gastric cancer than in normal gastric mucosa.Further analysis with clinicopathologic data showed that the relative higher S100P expression strongly correlated with tumor infiltration depth (P=0.006) and tumor size (P=0.001).Meanwhile, the expression of S100P between RNA and protein level (results from immunohistochemistry) was closely correlated (P=0.024).But it can not be used as a dependent prognosis factor. Conclusion S100P was down-regulated in gastric cancer；S100P expression correlated with tumor infiltration depth and tumor size；Down-regulated expression of S100P can be used as a supporting marker for prognosis prediction.
ObjectiveTo investigate the expression of S100P on RNA and protein levels in relation to clinicopathological features with gastric cancer patients. Methods Expression of S100P in primary gastric cancer (n=93) was analyzed using immunohistochemistry.Then, immunoblotting and RT-PCR were conducted on part of immunohistochemical-positively stained gastric cancer tissues and matched gastric mucosas. Results The immunohistochemical staining for S100P was predominantly nuclear with some cytoplasmic staining in normal gastric mucosa.As a result, 52.7% (49/93) of primary tumors and almost all the normal gastric mucosa were stained positively and S100P showed down-regulated expression in gastric cancer than in normal gastric mucosa.Further analysis with clinicopathologic data showed that the relative higher S100P expression strongly correlated with tumor infiltration depth (P=0.006) and tumor size (P=0.001).Meanwhile, the expression of S100P between RNA and protein level (results from immunohistochemistry) was closely correlated (P=0.024).But it can not be used as a dependent prognosis factor. Conclusion S100P was down-regulated in gastric cancer；S100P expression correlated with tumor infiltration depth and tumor size；Down-regulated expression of S100P can be used as a supporting marker for prognosis prediction.
2011, 38(04): 427-430.
DOI: 10.3971/j.issn.1000-8578.2011.04.016
Abstract：
ObjectiveTo study the pathological relative risk factors of postoperative radiation in gastric cancer after radical gastrectomy for clinic. Methods From Feb 2004 to Jan 2006, a total of 110 patients with newly diagnosed stomach carcinoma were enrolled in this study.All patients underwent gastrectomy with D1/D2 lymph node dissection and had complete postoperative pathologic analysis.Pathologically, all patients were confirmed with R0 disease.Univariate analysis was applied on the pathologic information and multivariate analysis was applied based on the univariate analysis. Results (1)Univariate analysis revealed that tumor diameter，histology，vascular invasion，lymphatic vessel invasion and neural invasion were correlated with T/N classification.Multivariate analysis revealed that vascular invasion and lymphatic vessel invasion were associated with T classification and lymphatic vessel invasion was associated with N classification.(2) For T and N classifications, lymphatic vessel invasion was strongly relevant factor. Conclusion For T and N classifications, lymphatic vessel invasion was strongly relevant factor.For patients with pathologically confirmed lymphatic vessel invasion, postoperative adjuvant radiotherapy is recommended.
ObjectiveTo study the pathological relative risk factors of postoperative radiation in gastric cancer after radical gastrectomy for clinic. Methods From Feb 2004 to Jan 2006, a total of 110 patients with newly diagnosed stomach carcinoma were enrolled in this study.All patients underwent gastrectomy with D1/D2 lymph node dissection and had complete postoperative pathologic analysis.Pathologically, all patients were confirmed with R0 disease.Univariate analysis was applied on the pathologic information and multivariate analysis was applied based on the univariate analysis. Results (1)Univariate analysis revealed that tumor diameter，histology，vascular invasion，lymphatic vessel invasion and neural invasion were correlated with T/N classification.Multivariate analysis revealed that vascular invasion and lymphatic vessel invasion were associated with T classification and lymphatic vessel invasion was associated with N classification.(2) For T and N classifications, lymphatic vessel invasion was strongly relevant factor. Conclusion For T and N classifications, lymphatic vessel invasion was strongly relevant factor.For patients with pathologically confirmed lymphatic vessel invasion, postoperative adjuvant radiotherapy is recommended.
2011, 38(04): 431-433.
DOI: 10.3971/j.issn.1000-8578.2011.04.017
Abstract：
ObjectiveTo explore the relationship of angiopoietins (Angs) with multiple myeloma(MM) and its clinical significance. Methods The serum concentration of Angs in different periods was detected by double-antibody sandwich enzyme-linked immunosorbent assays technique in 60 cases of MM patients and 20 controls.The associations between the serum cytokines levels with MM stages(Ⅱ～ Ⅲ), tumor load and the curative effect were observed,respectively. Results Pretreatment MM had significantly higher serum Ang-2 levels than controls.Serum Ang-1 levels in pretreatment MM had no significant difference compared with controls.Pretreatment serum Ang -2 levels were significantly lower in stage Ⅱ than in stage Ⅲ of MM.There weas significant difference between the different tumor load.In the patients responding to chemotherapy,Ang-2 serum levels was significantly decreased, while serum Ang-1 levels was no significant difference. In the patients who did not achieve a remission, there was no significantly change in serum Ang-1 levels and serum Ang-2 levels. Conclusion Serum levels of Ang-2 detection in MM patients is a certain extent significance to study of the pathogenesis of MM, the condition evaluation, efficacy monitoring, and outcome of prediction.
ObjectiveTo explore the relationship of angiopoietins (Angs) with multiple myeloma(MM) and its clinical significance. Methods The serum concentration of Angs in different periods was detected by double-antibody sandwich enzyme-linked immunosorbent assays technique in 60 cases of MM patients and 20 controls.The associations between the serum cytokines levels with MM stages(Ⅱ～ Ⅲ), tumor load and the curative effect were observed,respectively. Results Pretreatment MM had significantly higher serum Ang-2 levels than controls.Serum Ang-1 levels in pretreatment MM had no significant difference compared with controls.Pretreatment serum Ang -2 levels were significantly lower in stage Ⅱ than in stage Ⅲ of MM.There weas significant difference between the different tumor load.In the patients responding to chemotherapy,Ang-2 serum levels was significantly decreased, while serum Ang-1 levels was no significant difference. In the patients who did not achieve a remission, there was no significantly change in serum Ang-1 levels and serum Ang-2 levels. Conclusion Serum levels of Ang-2 detection in MM patients is a certain extent significance to study of the pathogenesis of MM, the condition evaluation, efficacy monitoring, and outcome of prediction.
2011, 38(04): 434-436.
DOI: 10.3971/j.issn.1000-8578.2011.04.018
Abstract：
ObjectiveTo investigate the diagnosis value of tumor markers (CYFRA21-1, CEA, NSE and CA153) in malignant pleural effusions. Methods 108 cases with malignant pleural effusions and 90 cases with benign pleural effusions were detected by radio immunoassay and ELISA, and then the results were compared. Results The four item of cancerous pleural effusion group were markedly higher than those of the benign ones(P<0.01).The sensitivity, specificity and accuracy rating of the combined detection of targets for diagnostician malignant pleural effusion were markedly higher. Conclusion The combined detection of CYFRA21 -1，CEA，NSE and CA153 has specific value in the diagnosis for malignant pleural effusions.
ObjectiveTo investigate the diagnosis value of tumor markers (CYFRA21-1, CEA, NSE and CA153) in malignant pleural effusions. Methods 108 cases with malignant pleural effusions and 90 cases with benign pleural effusions were detected by radio immunoassay and ELISA, and then the results were compared. Results The four item of cancerous pleural effusion group were markedly higher than those of the benign ones(P<0.01).The sensitivity, specificity and accuracy rating of the combined detection of targets for diagnostician malignant pleural effusion were markedly higher. Conclusion The combined detection of CYFRA21 -1，CEA，NSE and CA153 has specific value in the diagnosis for malignant pleural effusions.
2011, 38(04): 437-439.
DOI: 10.3971/j.issn.1000-8578.2011.04.019
Abstract：
ObjectiveTo assess the role of kilovoltage cone-beam CT (kVCBCT) in determining individual ITV for lung cancer patient. Methods Fourteen patients pathologically confirmed peripheral non-small cell lung cancer were entered into this study. One conventional planning CT and three kVCBCT scans were performed during quiet respiration for each patient.kVCBCT were all aligned to the conventional planning CT.After contouring GTV/IGTV, 5 mm margin was performed to generate CTV/ITV.The volume of ITV-p (generated from conventional planning CT) and ITV-c (generated from kVCBCT) were compared.Overlap volume of ITV-p and ITV-c were documented.The volume of CTV and ITV-c were compared with each other.The reproducibility of the kVCBCT was evaluated using the ratio of COM/SUM. Results The volume of ITV-p and ITV-c had statistics significant difference (P<0.0001).The overlap volume of ITV-p and ITV-c was 67.1 cc.The volume of CTV and ITV-c had statistics significant difference (P=0.005).The COM/SUM ratio of three ITVs which were defined from different kVCBCT scans was 63.0%±8.2%. Conclusion KVCBCT plays an important role in capturing the tumor movement and determine the individual ITV for lung cancer patient.
ObjectiveTo assess the role of kilovoltage cone-beam CT (kVCBCT) in determining individual ITV for lung cancer patient. Methods Fourteen patients pathologically confirmed peripheral non-small cell lung cancer were entered into this study. One conventional planning CT and three kVCBCT scans were performed during quiet respiration for each patient.kVCBCT were all aligned to the conventional planning CT.After contouring GTV/IGTV, 5 mm margin was performed to generate CTV/ITV.The volume of ITV-p (generated from conventional planning CT) and ITV-c (generated from kVCBCT) were compared.Overlap volume of ITV-p and ITV-c were documented.The volume of CTV and ITV-c were compared with each other.The reproducibility of the kVCBCT was evaluated using the ratio of COM/SUM. Results The volume of ITV-p and ITV-c had statistics significant difference (P<0.0001).The overlap volume of ITV-p and ITV-c was 67.1 cc.The volume of CTV and ITV-c had statistics significant difference (P=0.005).The COM/SUM ratio of three ITVs which were defined from different kVCBCT scans was 63.0%±8.2%. Conclusion KVCBCT plays an important role in capturing the tumor movement and determine the individual ITV for lung cancer patient.
2011, 38(04): 440-443.
DOI: 10.3971/j.issn.1000-8578.2011.04.020
Abstract：
ObjectiveTo explore the effect and complications of all course three dimensional conformal radiotherapy(3DCRT) combined with intracavitary brachytherapy in the treatment of cervical cancer．MethodsSixty cervical cancer patients were randomly divided randomly into two groups. First, all patients accepted 3D conformal radiotherapy in the 3DCRT group， when whole pelvic received 40Gy，a planning CT scan of each patient was obtained.And second 3DCRT therapy plans were taken.Then continued to irradiate with 50Gy.Finally, 3DCRT boost local involved volumes to the total dose of 60Gy.When 3DCRT combined with intracavitary branchtherapy，the dose of branchytherapy to point A was 30Gy/5fraction.In the conventional group after a total tumour dose of 40Gy was delivered by whole pelvic irradiation, the four-field technique was used to irradiate the parametrial tissue and regional nodes (median dose of 10Gy), the invovlved volumes were boosted to 60Gy and combined with intracavitary branchtherapy，(the dose of branchytherapy to point A was (30~36)Gy/(5~6)fraction).In two groups two cycle chemotherapy were given concurrently with radiotherapy in the first week and the last week, respectively. ResultsThe 1,2,3-year survival rate for 3DCRT group and conventional group were 96.7%,93.3％,90.0% and 86.6％,76.7％,and 70% respectively(P=0.04,P=0.02 and P=0.02).There was significant difference.The toxic effects were compared in two group, except the Ⅰ～Ⅱ grade rectal and bladder reaction and pelvic fibrosis which was lower in the 3DCRT group (P=0.007,P=0.006 and P=0.015), the side effects were similar and well tolerated in two groups. ConclusionThe all course three dimensional conformal radiotherapy combined with intracavitary brachytherapy can be considered as an effective and feasible approach to cervical cancer and may significantly improve the survival rate and reduce the late toxicity.
ObjectiveTo explore the effect and complications of all course three dimensional conformal radiotherapy(3DCRT) combined with intracavitary brachytherapy in the treatment of cervical cancer．MethodsSixty cervical cancer patients were randomly divided randomly into two groups. First, all patients accepted 3D conformal radiotherapy in the 3DCRT group， when whole pelvic received 40Gy，a planning CT scan of each patient was obtained.And second 3DCRT therapy plans were taken.Then continued to irradiate with 50Gy.Finally, 3DCRT boost local involved volumes to the total dose of 60Gy.When 3DCRT combined with intracavitary branchtherapy，the dose of branchytherapy to point A was 30Gy/5fraction.In the conventional group after a total tumour dose of 40Gy was delivered by whole pelvic irradiation, the four-field technique was used to irradiate the parametrial tissue and regional nodes (median dose of 10Gy), the invovlved volumes were boosted to 60Gy and combined with intracavitary branchtherapy，(the dose of branchytherapy to point A was (30~36)Gy/(5~6)fraction).In two groups two cycle chemotherapy were given concurrently with radiotherapy in the first week and the last week, respectively. ResultsThe 1,2,3-year survival rate for 3DCRT group and conventional group were 96.7%,93.3％,90.0% and 86.6％,76.7％,and 70% respectively(P=0.04,P=0.02 and P=0.02).There was significant difference.The toxic effects were compared in two group, except the Ⅰ～Ⅱ grade rectal and bladder reaction and pelvic fibrosis which was lower in the 3DCRT group (P=0.007,P=0.006 and P=0.015), the side effects were similar and well tolerated in two groups. ConclusionThe all course three dimensional conformal radiotherapy combined with intracavitary brachytherapy can be considered as an effective and feasible approach to cervical cancer and may significantly improve the survival rate and reduce the late toxicity.
2011, 38(04): 444-446.
DOI: 10.3971/j.issn.1000-8578.2011.04.021
Abstract：
ObjectiveTo evaluate the efficacy of using gefitinib as maintenance therapy after chemotherapy. Methods Patients achieved partial response or disease stabilization following the last chemotherapy was devided into maintenance group.Gefitinib was administered at the dose of 250 mg/d. Results From Jan. 2006 to Jun. 2008, we enrolled 40 patients.Response rate to gefitinibwas 52.38% (11/21) in maintenance group and 21.05% (4/19) in control group(P<0.05),respectively.In comparison with control group, patients in maintenance group had longer time to survival (median OS ： 16.19 months vs.7.90 months, (P<0.05).The toxicity profile was mild and there was no significant difference between two groups. Conclusion Maintenance therapy with gefitinib was feasible and safe, which produced significantly longer OS of elderly patients with advanced lung adenocarcinoma.
ObjectiveTo evaluate the efficacy of using gefitinib as maintenance therapy after chemotherapy. Methods Patients achieved partial response or disease stabilization following the last chemotherapy was devided into maintenance group.Gefitinib was administered at the dose of 250 mg/d. Results From Jan. 2006 to Jun. 2008, we enrolled 40 patients.Response rate to gefitinibwas 52.38% (11/21) in maintenance group and 21.05% (4/19) in control group(P<0.05),respectively.In comparison with control group, patients in maintenance group had longer time to survival (median OS ： 16.19 months vs.7.90 months, (P<0.05).The toxicity profile was mild and there was no significant difference between two groups. Conclusion Maintenance therapy with gefitinib was feasible and safe, which produced significantly longer OS of elderly patients with advanced lung adenocarcinoma.
2011, 38(04): 447-451.
DOI: 10.3971/j.issn.1000-8578.2011.04.022
Abstract：
ObjectiveTo compare the efficacy and side effects of high-intensity focused ultrasound (HIFU) with anhydrous alcohol injection guided by ultrasound on patients with portal vein tumor thrombosis (PVTT). Methods 86 patients with primary liver cancer (PLC) combined with PVTT in our hospital were retrospectively analyzed.Liver lesions were basically treated with transcatheter arterial chemoembolization (TACE), and HIFU or percutaneous anhydrous alcohol injection were sued as complimentary treatment to 52 cases (group A) and 34 cases (group B),respectively.Response rate (RR), clinical beneficial response (CBR), overall survival (OS) and side effects were evaluated between two groups. Results RR in group A and B were 40.38% vs. 41.18% (P=0.1493), CBR were 48.08% vs. 23.53%(P=0.0102)， median OS were 12.3 months (range：3.6~23.5) vs. 11.5 months (range： 4.2~21.3) (P=0.1504).Main side effects occurred in group A was upper gastrointestinal(GI) hemorrhage and liver area pain, while fever and hemorrhage under liver capsule occurred in group B.The occurance rates of side effects in group B were much higher than those in group A (P=0.0051). Conclusion HIFU and percutaneous anhydrous alcohol injection guided by ultrasound are both safe and effective approaches for therapy of PVTT.The short-term and long-term response was similar in two groups, while HIFU was superior to anhydrous alcohol injection on CBR and side effects.HIFU maybe a good choice patients with disordered liver function, ascites and intolerable to other methods.
ObjectiveTo compare the efficacy and side effects of high-intensity focused ultrasound (HIFU) with anhydrous alcohol injection guided by ultrasound on patients with portal vein tumor thrombosis (PVTT). Methods 86 patients with primary liver cancer (PLC) combined with PVTT in our hospital were retrospectively analyzed.Liver lesions were basically treated with transcatheter arterial chemoembolization (TACE), and HIFU or percutaneous anhydrous alcohol injection were sued as complimentary treatment to 52 cases (group A) and 34 cases (group B),respectively.Response rate (RR), clinical beneficial response (CBR), overall survival (OS) and side effects were evaluated between two groups. Results RR in group A and B were 40.38% vs. 41.18% (P=0.1493), CBR were 48.08% vs. 23.53%(P=0.0102)， median OS were 12.3 months (range：3.6~23.5) vs. 11.5 months (range： 4.2~21.3) (P=0.1504).Main side effects occurred in group A was upper gastrointestinal(GI) hemorrhage and liver area pain, while fever and hemorrhage under liver capsule occurred in group B.The occurance rates of side effects in group B were much higher than those in group A (P=0.0051). Conclusion HIFU and percutaneous anhydrous alcohol injection guided by ultrasound are both safe and effective approaches for therapy of PVTT.The short-term and long-term response was similar in two groups, while HIFU was superior to anhydrous alcohol injection on CBR and side effects.HIFU maybe a good choice patients with disordered liver function, ascites and intolerable to other methods.
2011, 38(04): 452-454.
DOI: 10.3971/j.issn.1000-8578.2011.04.023
Abstract：
ObjectiveTo evaluate the therapeutic efficacy and safety of transjugular intrahepatic portosystemic shunt (TIPS) for the treatment on variceal bleeding due to portal hypertension（PHT）of patients with hepatocellular carcinoma(HCC)，and to evaluate factors for case selection for TIPS. Methods 48 patients with HCC complicated variceal bleeding due to portal hypertension performed TIPS were retrospected.Of the 48 patients, the average age was 50.8 years.Related information after TIPS, such as treatment success, complications, causes of death and so on, were assessed.The data of survival time of the 48 patients after treatment was collected by follow-up.The Kaplan-Meier method was used to conduct the survival analyses. Results TIPS was successful in 48 cases, the mean portal vein pressure was reduced about 10.6 mmHg (1mmHg=0.133kPa).After TIPS, variceal bleeding was controlled, the ascites and the clinical symptoms were relieved.According to the follow-up, incidence rate of hepatic encephalopathy complicated with TIPS was 18.8% and rebleeding was 27.0%.The median survival time of the patients was about 5.0 months, and it shortened from Child-Pugh class A to C. Conclusion TIPS is one of effective and safe therapeutic methods to control variceal bleeding due to portal hypertension of patients with HCC.However, liver function is an important factor for case selection for TIPS.
ObjectiveTo evaluate the therapeutic efficacy and safety of transjugular intrahepatic portosystemic shunt (TIPS) for the treatment on variceal bleeding due to portal hypertension（PHT）of patients with hepatocellular carcinoma(HCC)，and to evaluate factors for case selection for TIPS. Methods 48 patients with HCC complicated variceal bleeding due to portal hypertension performed TIPS were retrospected.Of the 48 patients, the average age was 50.8 years.Related information after TIPS, such as treatment success, complications, causes of death and so on, were assessed.The data of survival time of the 48 patients after treatment was collected by follow-up.The Kaplan-Meier method was used to conduct the survival analyses. Results TIPS was successful in 48 cases, the mean portal vein pressure was reduced about 10.6 mmHg (1mmHg=0.133kPa).After TIPS, variceal bleeding was controlled, the ascites and the clinical symptoms were relieved.According to the follow-up, incidence rate of hepatic encephalopathy complicated with TIPS was 18.8% and rebleeding was 27.0%.The median survival time of the patients was about 5.0 months, and it shortened from Child-Pugh class A to C. Conclusion TIPS is one of effective and safe therapeutic methods to control variceal bleeding due to portal hypertension of patients with HCC.However, liver function is an important factor for case selection for TIPS.
2011, 38(04): 455-457.
DOI: 10.3971/j.issn.1000-8578.2011.04.024
Abstract：
ObjectiveTo investigate the therapeutic efficacy of 99Tc-MDP combining with 89Sr in alleviating pain of patients with osseous metastasis. Methods 89Sr 3mci was intravenously injected into 120 patients with osseous metastasis, after a week, 99Tc-MDP, 22mg/d, was intravenously injected for 10 days.One course of treatment was one month and was carried on 3 to 6 months；89Sr was used in every 3 to 4 months, three times at most. Results The pain was significant relieved in 107 patients, relieved in 8 patients,and inefficient in 5 patients.The effective power was 96% (relieve in 2 or 3 weeks)；The time extended more than a year and a half.Myelosuppression was occured in few patient;Some patients with destruction of bone were obviously recovered. Conclusion There are conspicuous effect of 99Tc-MDP combining with 89Sr in therapying patients with osseous metastasis and conspicuous recovery for destruction of bone induced by osseous metastasis；Patients with osseous metastasis should be treated with 99Tc-MDP combining with 89Sr at an early date.
ObjectiveTo investigate the therapeutic efficacy of 99Tc-MDP combining with 89Sr in alleviating pain of patients with osseous metastasis. Methods 89Sr 3mci was intravenously injected into 120 patients with osseous metastasis, after a week, 99Tc-MDP, 22mg/d, was intravenously injected for 10 days.One course of treatment was one month and was carried on 3 to 6 months；89Sr was used in every 3 to 4 months, three times at most. Results The pain was significant relieved in 107 patients, relieved in 8 patients,and inefficient in 5 patients.The effective power was 96% (relieve in 2 or 3 weeks)；The time extended more than a year and a half.Myelosuppression was occured in few patient;Some patients with destruction of bone were obviously recovered. Conclusion There are conspicuous effect of 99Tc-MDP combining with 89Sr in therapying patients with osseous metastasis and conspicuous recovery for destruction of bone induced by osseous metastasis；Patients with osseous metastasis should be treated with 99Tc-MDP combining with 89Sr at an early date.
2011, 38(04): 458-459.
DOI: 10.3971/j.issn.1000-8578.2011.04.025
Abstract：
ObjectiveTo study the 21 genotypes of human papilloma virus(HPV) and its clinical significance. Methods 1 540 females with cervical disease were carried through HPV typing in all 21 genotypes in our hospital,then comparing the different genotypes and clincal disease feature. Results The total positive rate was 31.8%(489/1 540),21 isoforms were checked out， HPV16,58,31 were seen mostly in high risk and HPV6,11 were seen mostly in low risk.Among the positive rates in different stage of years,the less than 40 years old group was the highest.Positive for HPV infection in cervical lesions and cervical carcinoma prevalence was significantly higher than that in the negative group. Conclusion Infection of HPV genotypes are related closely with cervical different-grade lesions,which may apply reasonable evidences for screening,prevention and cure of cervical carcinoma.
ObjectiveTo study the 21 genotypes of human papilloma virus(HPV) and its clinical significance. Methods 1 540 females with cervical disease were carried through HPV typing in all 21 genotypes in our hospital,then comparing the different genotypes and clincal disease feature. Results The total positive rate was 31.8%(489/1 540),21 isoforms were checked out， HPV16,58,31 were seen mostly in high risk and HPV6,11 were seen mostly in low risk.Among the positive rates in different stage of years,the less than 40 years old group was the highest.Positive for HPV infection in cervical lesions and cervical carcinoma prevalence was significantly higher than that in the negative group. Conclusion Infection of HPV genotypes are related closely with cervical different-grade lesions,which may apply reasonable evidences for screening,prevention and cure of cervical carcinoma.
2011, 38(04): 460-461.
DOI: 10.3971/j.issn.1000-8578.2011.04.026
2011, 38(04): 474-475.
DOI: 10.3971/j.issn.1000-8578.2011.04.032
2011, 38(04): 476-478.
DOI: 10.3971/j.issn.1000-8578.2011.04.033
