2007 Vol. 34 No. 06
2007, 34(06): 395-398.
DOI: 10.3971/j.issn.1000-8578.695
Abstract：
Objective Monoclonal antibodies which inhibit lung cancer may be candidate drugs for the antimalignancy therapy. Methods Malignant cells were separated form lung carcinoma tissues to immunize Balb/c mouse. The spleen cells of the immunized mouse were fused with SP2/0 cells and culture on the methyl cellulose. Antibodies of each clone were screened by immunofluorescence, immunohistochemistry, ELISA, cell proliferation assay, tumor cell invasion assay and adhesion assay. Functional antibodies were also studied by tumor treatment experiments in vivo. Results One thousand five hundred and seventy-three monoclonal antibody clones were obtained by the fuse of spleen cells with SP2/0 cells. Forty-one clones could significantly suppress the lung cancer cell proliferation, 24 monoclonal antibody could inhibit tumor cell invasion in Mat rigel, and 15 monoclonal antibody could decrease the adherence between lung cancer cells and Collagen I. I n vivo assay further demonst rated that two of these functional antibodies could significantly inhibit lung tumor growth in mice. Conclusion By using functional antibody library, we successfully obtained functional monoclonal antibodies which suppressed the lung cancer cells in vitro and in vivo. These antibodies may be applied in anti-tumor therapy of lung carcinoma.
Objective Monoclonal antibodies which inhibit lung cancer may be candidate drugs for the antimalignancy therapy. Methods Malignant cells were separated form lung carcinoma tissues to immunize Balb/c mouse. The spleen cells of the immunized mouse were fused with SP2/0 cells and culture on the methyl cellulose. Antibodies of each clone were screened by immunofluorescence, immunohistochemistry, ELISA, cell proliferation assay, tumor cell invasion assay and adhesion assay. Functional antibodies were also studied by tumor treatment experiments in vivo. Results One thousand five hundred and seventy-three monoclonal antibody clones were obtained by the fuse of spleen cells with SP2/0 cells. Forty-one clones could significantly suppress the lung cancer cell proliferation, 24 monoclonal antibody could inhibit tumor cell invasion in Mat rigel, and 15 monoclonal antibody could decrease the adherence between lung cancer cells and Collagen I. I n vivo assay further demonst rated that two of these functional antibodies could significantly inhibit lung tumor growth in mice. Conclusion By using functional antibody library, we successfully obtained functional monoclonal antibodies which suppressed the lung cancer cells in vitro and in vivo. These antibodies may be applied in anti-tumor therapy of lung carcinoma.
2007, 34(06): 399-401.
DOI: 10.3971/j.issn.1000-8578.684
Abstract：
Objective To study the localization and expression levels of MTA1 protein in esophageal squamous cell carcinoma and the relationship between MTA1 expression and the development and metastasis of esophageal squamous cell carcinoma. Methods Immunohistochemistry staining was adopted in tissue array analysis. Results MTA1 was located mainly in the nucleus in the primary esophageal squamous cell carcinomas, but in the cells from metastatic carcinoma, the cytoplasmic staining of MTA1 protein located in mucleus and cytoplasm. The difference between normal esophageal squamous cells and dysphasia cells, invasive carcinoma cells was significant ( P < 0. 01) . With the development of esophageal squamous cell carcinoma, the MTA1 expression increased with ectopic location. Conclusion There is a st rong relationship between MTA1 overexpression and invasion metastasis of esophageal squamous cell carcinoma, indicating an important role of MTA1 in the development of esophageal squamous cell carcinoma. The MTA1 deregulation may be an accessory indicator for assessing the invasive and metastatic potential of esophageal squamous cell carcinomas.
Objective To study the localization and expression levels of MTA1 protein in esophageal squamous cell carcinoma and the relationship between MTA1 expression and the development and metastasis of esophageal squamous cell carcinoma. Methods Immunohistochemistry staining was adopted in tissue array analysis. Results MTA1 was located mainly in the nucleus in the primary esophageal squamous cell carcinomas, but in the cells from metastatic carcinoma, the cytoplasmic staining of MTA1 protein located in mucleus and cytoplasm. The difference between normal esophageal squamous cells and dysphasia cells, invasive carcinoma cells was significant ( P < 0. 01) . With the development of esophageal squamous cell carcinoma, the MTA1 expression increased with ectopic location. Conclusion There is a st rong relationship between MTA1 overexpression and invasion metastasis of esophageal squamous cell carcinoma, indicating an important role of MTA1 in the development of esophageal squamous cell carcinoma. The MTA1 deregulation may be an accessory indicator for assessing the invasive and metastatic potential of esophageal squamous cell carcinomas.
2007, 34(06): 402-404.
DOI: 10.3971/j.issn.1000-8578.3252
Abstract：
Objective In order to seek for a therapeutic approach to the diseases caused by HPV or cervix cancer, we const ructed antisense eukaryotic fluorescent expression vector of human papillomavirus ( HPV) 18 E6 E7 and detected their expression in HeLa cervical carcinoma cells. Methods HPV18 E6E7 716bp was amplified the by PCR with the full2length HPV-18 cDNA clone vector as the template. Then the PCR product was inserted into eukaryotic fluorescent expressing vector pEGFP in antisense orientation by DNA recombinant technology and further transfected into HeLa cervical carcinoma cells. Expression of HPV18 E6/E7 in t ransfectant s was examined by the fluorescence microscope, RT-PCR and Western blot . Results The fluorescence eukaryotic expression vector carrying antisense HPV18 E6 E7 gene fragment was successfully const ructed. At 48h af ter t ransfection of recombinant plasmid, bright green fluorescence was visible and the E6/E7 mRNA and proteins were obviously down regulated in the HeLa cervical carcinoma cells. Conclusion Delivery of antisense HPV18 E6 E7 can effectively suppress the expression of E6/E7 oncogenes and is potentially a new approach to t reat HPV infection and cervix cancer.
Objective In order to seek for a therapeutic approach to the diseases caused by HPV or cervix cancer, we const ructed antisense eukaryotic fluorescent expression vector of human papillomavirus ( HPV) 18 E6 E7 and detected their expression in HeLa cervical carcinoma cells. Methods HPV18 E6E7 716bp was amplified the by PCR with the full2length HPV-18 cDNA clone vector as the template. Then the PCR product was inserted into eukaryotic fluorescent expressing vector pEGFP in antisense orientation by DNA recombinant technology and further transfected into HeLa cervical carcinoma cells. Expression of HPV18 E6/E7 in t ransfectant s was examined by the fluorescence microscope, RT-PCR and Western blot . Results The fluorescence eukaryotic expression vector carrying antisense HPV18 E6 E7 gene fragment was successfully const ructed. At 48h af ter t ransfection of recombinant plasmid, bright green fluorescence was visible and the E6/E7 mRNA and proteins were obviously down regulated in the HeLa cervical carcinoma cells. Conclusion Delivery of antisense HPV18 E6 E7 can effectively suppress the expression of E6/E7 oncogenes and is potentially a new approach to t reat HPV infection and cervix cancer.
2007, 34(06): 405-408.
DOI: 10.3971/j.issn.1000-8578.2750
Abstract：
Objective To construct prokaryotic recombinant expression plasmid pGEX-4 T-1-MA GE-3 and analysis of it s expression in BL21 E. coli. Methods MAGE-3 aim gene was obtained by RT-PCR. The target gene was orientating cloned into p GEM-T Easy and subclone into p GEX-4 T-1 vector by DNA recombinant technical. Position clones were t ransformed into BL21. And then they were induced with IPTG, identified by 12 % SDS-PA GE elect rophoresis and Western-blot . Results MAGE-3 fragment (349bp) was amplified and the prokaryotic recombinant expression plasmid p GEX-4 T-1-MAGE-3 was correctly constructed. DNA sequecing results showed that the sequence of MAGE-3 gene fragment in position clones is the completely same as the sequence of the GenBank public. The 35kD fusion protein was observed in BL21 E. coli and verfied that it is the target protein. Conclusion Prokaryotic recombinant expression plasmid p GEX-4 T-1-MA GE-3 was successfully const ructed and the fusion protein was expressed by induced. These lay a foundation for providing antigen which will be used as peptide vaccine and specific diagnosis reagent based on MAGE-3 aim gene, and also provide an experimental basis for further research.
Objective To construct prokaryotic recombinant expression plasmid pGEX-4 T-1-MA GE-3 and analysis of it s expression in BL21 E. coli. Methods MAGE-3 aim gene was obtained by RT-PCR. The target gene was orientating cloned into p GEM-T Easy and subclone into p GEX-4 T-1 vector by DNA recombinant technical. Position clones were t ransformed into BL21. And then they were induced with IPTG, identified by 12 % SDS-PA GE elect rophoresis and Western-blot . Results MAGE-3 fragment (349bp) was amplified and the prokaryotic recombinant expression plasmid p GEX-4 T-1-MAGE-3 was correctly constructed. DNA sequecing results showed that the sequence of MAGE-3 gene fragment in position clones is the completely same as the sequence of the GenBank public. The 35kD fusion protein was observed in BL21 E. coli and verfied that it is the target protein. Conclusion Prokaryotic recombinant expression plasmid p GEX-4 T-1-MA GE-3 was successfully const ructed and the fusion protein was expressed by induced. These lay a foundation for providing antigen which will be used as peptide vaccine and specific diagnosis reagent based on MAGE-3 aim gene, and also provide an experimental basis for further research.
2007, 34(06): 409-411.
DOI: 10.3971/j.issn.1000-8578.1527
Abstract：
Objective To const ruct prokaryotic expression vectors for Livinαand Livinβand to obtained the fusion protein of p ET32a ( + )-livinα and p ET32a ( + )2livinβ. Methods Total RNA of Hela cell was extracted. The full-length cDNA of Livin isoforms was gained by RT-PCR. Then inserted into p ET32a ( + ) vector and const ructed the recombinant plasmids p ET32a ( + )2livinα/ DH5α and p ET32a ( + ) / livinβ/DH5α, sequencing was performed to guarantee correct sequence insertion for the isoforms Livinα and Livinβ. Reconst ructed the recombinant plasmids p ET32a ( + )-livinα/ BL21 and p ET32a ( + ) / livinβ/BL21, the p ET32a ( + )-livinα/ BL21 and p ET32a ( + )-livinβ/ BL21 plasmids was induced af ter 4. 5 hours by IPTG, and the value of A600nmwas 0. 6 in LB medium. Expression of the p ET32a ( + )-livinα/ BL21 and p ET32a ( + )-livinβ/ BL21 were analyzed by SDS-PA GE . Results Full-length cDNA of Livinαand Livinβ was cloned respectively and subcloned into p ET32a ( + ) successfully. Fusion protein of positive recombinants were gained by p ET32a ( + ) prokaryotic expression system. Conclusion The construction of prokaryotic expression vector for Livinαand Livinβand validation of expression in cells provide basis for further research on functions of Livinαand Livinβand their anti-apoptosis effect in cancer cell.
Objective To const ruct prokaryotic expression vectors for Livinαand Livinβand to obtained the fusion protein of p ET32a ( + )-livinα and p ET32a ( + )2livinβ. Methods Total RNA of Hela cell was extracted. The full-length cDNA of Livin isoforms was gained by RT-PCR. Then inserted into p ET32a ( + ) vector and const ructed the recombinant plasmids p ET32a ( + )2livinα/ DH5α and p ET32a ( + ) / livinβ/DH5α, sequencing was performed to guarantee correct sequence insertion for the isoforms Livinα and Livinβ. Reconst ructed the recombinant plasmids p ET32a ( + )-livinα/ BL21 and p ET32a ( + ) / livinβ/BL21, the p ET32a ( + )-livinα/ BL21 and p ET32a ( + )-livinβ/ BL21 plasmids was induced af ter 4. 5 hours by IPTG, and the value of A600nmwas 0. 6 in LB medium. Expression of the p ET32a ( + )-livinα/ BL21 and p ET32a ( + )-livinβ/ BL21 were analyzed by SDS-PA GE . Results Full-length cDNA of Livinαand Livinβ was cloned respectively and subcloned into p ET32a ( + ) successfully. Fusion protein of positive recombinants were gained by p ET32a ( + ) prokaryotic expression system. Conclusion The construction of prokaryotic expression vector for Livinαand Livinβand validation of expression in cells provide basis for further research on functions of Livinαand Livinβand their anti-apoptosis effect in cancer cell.
2007, 34(06): 412-415.
DOI: 10.3971/j.issn.1000-8578.3219
Abstract：
Objective To predict the HLA-A2/A3-rest ricted CTL epitopes of COX-2 and MAGE-4 hyperexpressed in esophageal carcinoma ( ECC) . Methods The HLA-A *0201/A3 rest ricted CTL epitopes of objective antigens were predicted by SYFPEITHI prediction method combined with the MHCPred and NetChop 3. 0. All peptides were compared with reported CTL epitopes. Results The SYFPEITHI prediction values of all possible nonamers of the two proteins sequence were added together and the over 20-score peptides were chosen for further analysis in primary prediction. 42 candidates of CTL epitopes (nonamers) derived from the primary prediction results were selected by analyzing with the MHCPred and NetChop3. 0 methods. Conclusion All these peptides have not been reported. The 42 candidates of CTL epitopes will benefit the identification of CTL epitopes by experiment . These nonamers may be useful in the design of therapeutic peptide vaccine for ECC and as immunotherapeutic st rategies against ECC after identified by immunology experiment . The peptides MAGE-4 22-30、202-210、286-294 and COX-2 479-487 have the potential cross-immunity with HLA-A2 and HLA-A3 supertype, which will provide candidates for efficient and broad-spectrum vaccine.
Objective To predict the HLA-A2/A3-rest ricted CTL epitopes of COX-2 and MAGE-4 hyperexpressed in esophageal carcinoma ( ECC) . Methods The HLA-A *0201/A3 rest ricted CTL epitopes of objective antigens were predicted by SYFPEITHI prediction method combined with the MHCPred and NetChop 3. 0. All peptides were compared with reported CTL epitopes. Results The SYFPEITHI prediction values of all possible nonamers of the two proteins sequence were added together and the over 20-score peptides were chosen for further analysis in primary prediction. 42 candidates of CTL epitopes (nonamers) derived from the primary prediction results were selected by analyzing with the MHCPred and NetChop3. 0 methods. Conclusion All these peptides have not been reported. The 42 candidates of CTL epitopes will benefit the identification of CTL epitopes by experiment . These nonamers may be useful in the design of therapeutic peptide vaccine for ECC and as immunotherapeutic st rategies against ECC after identified by immunology experiment . The peptides MAGE-4 22-30、202-210、286-294 and COX-2 479-487 have the potential cross-immunity with HLA-A2 and HLA-A3 supertype, which will provide candidates for efficient and broad-spectrum vaccine.
2007, 34(06): 416-419.
DOI: 10.3971/j.issn.1000-8578.1668
Abstract：
Objective To investigate the association of the interleukin-1B ( IL-1B) gene polymorphism with gastric cancer in Henyang Chinese population, and to study which type may be the sensitive genotype to gastric cancer in patients with helicobacter pylori ( HP) infection. Methods For 52 patients with gastric cancer and 55 patients with chronic gastritis., the polymorphism of the IL-1B gene was studied by using polymerase chain reaction-rest riction f ragment length polymorphism ( PCR-RFL P) analysis, C/ C、T/ T genotype were sequenced. The infections of HP were determined by RU T and PCR assay. Results The frequencies of IL-1B-31 T, IL-1B-511 T, IL-1B-31 T/ T and IL-1B-511 T/ T genotype were higher in gastric cancer group than in chronic gast ritis group ( P < 0. 05), with odds ratios (ORs) of 1. 97 (95 %CI = 1. 15～3. 59), 2. 52 (95 %CI = 1. 45～4. 39), 2. 71 (95 %CI = 1. 10～6. 66) and 3. 33 (95 %CI = 1. 14～9. 73), respectively. In patient s with HP infections, the IL-1B-511 T and IL-1B-511 T/ T were higher in gastric cancer group than in chronic gastritis group ( P < 0. 05), with odds ratios of 2. 16 (95 %CI = 1. 10～4. 23) and 3. 43 (95 %CI = 1. 01～11. 62), respectively. Conclusion In the HengYang Chinese population, the IL-1B-31 T/ T and IL-1B-511 T/ T genotypes may be associated with the high risk of gastric cancer, and IL-1B-511 T/ T may be the sensitive genotype of gastric cancer in patients with HP infection.
Objective To investigate the association of the interleukin-1B ( IL-1B) gene polymorphism with gastric cancer in Henyang Chinese population, and to study which type may be the sensitive genotype to gastric cancer in patients with helicobacter pylori ( HP) infection. Methods For 52 patients with gastric cancer and 55 patients with chronic gastritis., the polymorphism of the IL-1B gene was studied by using polymerase chain reaction-rest riction f ragment length polymorphism ( PCR-RFL P) analysis, C/ C、T/ T genotype were sequenced. The infections of HP were determined by RU T and PCR assay. Results The frequencies of IL-1B-31 T, IL-1B-511 T, IL-1B-31 T/ T and IL-1B-511 T/ T genotype were higher in gastric cancer group than in chronic gast ritis group ( P < 0. 05), with odds ratios (ORs) of 1. 97 (95 %CI = 1. 15～3. 59), 2. 52 (95 %CI = 1. 45～4. 39), 2. 71 (95 %CI = 1. 10～6. 66) and 3. 33 (95 %CI = 1. 14～9. 73), respectively. In patient s with HP infections, the IL-1B-511 T and IL-1B-511 T/ T were higher in gastric cancer group than in chronic gastritis group ( P < 0. 05), with odds ratios of 2. 16 (95 %CI = 1. 10～4. 23) and 3. 43 (95 %CI = 1. 01～11. 62), respectively. Conclusion In the HengYang Chinese population, the IL-1B-31 T/ T and IL-1B-511 T/ T genotypes may be associated with the high risk of gastric cancer, and IL-1B-511 T/ T may be the sensitive genotype of gastric cancer in patients with HP infection.
2007, 34(06): 420-422.
DOI: 10.3971/j.issn.1000-8578.567
Abstract：
Objective To explore antitumor protective immunity induced by dendritic cells based vaccine in humanised nude mice model. Methods Immature DCs generated from peripheral blood mononuclear cells were transfected with gastric carcinoma total RNA in vitro. A humanized nude mice model was established by intraperitoneal (i. p. ) injection of human peripheral blood T-lymphocytes ( HuPBTL ) . The mice were randomly divided into DCRNA group, DCs group and PBS group of 5 mice each. The mice were immunized s. c. with DCRNA or DCs and PBS, respectively, twice, at weekly intervals. Three days after the last immunization mice were challenged s. c. with BGC-823 cells. The CD4 + and CD8 + T cell subpopulations of human T-lymphocytes from peripheral blood of humanised nude mice were detected by flow cytometry and graft versus host disease ( GVHD) were observed. Tumor growth, tumor volume, tumor weight and tumor inhibition rate were observed. Production of cytokine IL-12 and IFN-γ were detected by ELISA kit . Results The CD4 + and CD8 + T cells subpopulations of human T2lymphocytes from peripheral blood of humanised nude mice were found in each group. GV HD was not occurrence in each group. Overall tumor volume in DCRNA group mice was significantly lower than those of DCs group and PBS group ( P < 0. 05) . Tumor weight in DCRNA group was significantly lighter than those of DCs group and PBS group ( P = 0. 0452, P = 0. 0004), but this difference was not statistically significant between DCs group and PBS group ( P = 0. 0618) . Inhibition tumor rate for DCRNA group (53. 7 %) was higher than that for DCs group (25. 1 %) ( P < 0. 05) . Production of cytokine IL-12 and IFN-γ in DCRNA group were significantly increased relatived to DCs group and PBS group ( P < 0. 05, P < 0. 01) . Conclusion A humanised nude mouse model was successfully established by i. p. injection of HuPBTL. DCs based vaccine is capable of inducing enhanced antitumor protective immunity in vivo.
Objective To explore antitumor protective immunity induced by dendritic cells based vaccine in humanised nude mice model. Methods Immature DCs generated from peripheral blood mononuclear cells were transfected with gastric carcinoma total RNA in vitro. A humanized nude mice model was established by intraperitoneal (i. p. ) injection of human peripheral blood T-lymphocytes ( HuPBTL ) . The mice were randomly divided into DCRNA group, DCs group and PBS group of 5 mice each. The mice were immunized s. c. with DCRNA or DCs and PBS, respectively, twice, at weekly intervals. Three days after the last immunization mice were challenged s. c. with BGC-823 cells. The CD4 + and CD8 + T cell subpopulations of human T-lymphocytes from peripheral blood of humanised nude mice were detected by flow cytometry and graft versus host disease ( GVHD) were observed. Tumor growth, tumor volume, tumor weight and tumor inhibition rate were observed. Production of cytokine IL-12 and IFN-γ were detected by ELISA kit . Results The CD4 + and CD8 + T cells subpopulations of human T2lymphocytes from peripheral blood of humanised nude mice were found in each group. GV HD was not occurrence in each group. Overall tumor volume in DCRNA group mice was significantly lower than those of DCs group and PBS group ( P < 0. 05) . Tumor weight in DCRNA group was significantly lighter than those of DCs group and PBS group ( P = 0. 0452, P = 0. 0004), but this difference was not statistically significant between DCs group and PBS group ( P = 0. 0618) . Inhibition tumor rate for DCRNA group (53. 7 %) was higher than that for DCs group (25. 1 %) ( P < 0. 05) . Production of cytokine IL-12 and IFN-γ in DCRNA group were significantly increased relatived to DCs group and PBS group ( P < 0. 05, P < 0. 01) . Conclusion A humanised nude mouse model was successfully established by i. p. injection of HuPBTL. DCs based vaccine is capable of inducing enhanced antitumor protective immunity in vivo.
2007, 34(06): 423-424.
DOI: 10.3971/j.issn.1000-8578.1303
Abstract：
Objective To examine the expressions of FHIT and PTEN in gliomas, and to investigate the relationship between their expressions and the degree of tumor malignancy. Methods SP Immunohistochemical staining was used to examine the expressions of FHIT, PTEN in 80 gliomas and in 5 normal brain tissues. Results FHIT was located in the plasma of glioma cells, PTEN was located in the cellular nuclei of glioma cells, total positive rates were 47. 5 % (38/ 80), 55. 0 % (44/ 80) respectively. Expression rates of FHIT and PTEN in normal brain tissues were 80 % (4/ 5) . There was a statistically significant difference in the expression of FHIT and PTEN between normal brain tissues and different grades of gliomas ( P < 0. 05) . Both of the expressions of FHIT and PTEN weakened with the rise in the degree of glioma malignancy. Conclusion Expressions of FHIT and PTEN in glioma cells are diminishing or absent, their cooperative operation promotes generation and advance of glioma.
Objective To examine the expressions of FHIT and PTEN in gliomas, and to investigate the relationship between their expressions and the degree of tumor malignancy. Methods SP Immunohistochemical staining was used to examine the expressions of FHIT, PTEN in 80 gliomas and in 5 normal brain tissues. Results FHIT was located in the plasma of glioma cells, PTEN was located in the cellular nuclei of glioma cells, total positive rates were 47. 5 % (38/ 80), 55. 0 % (44/ 80) respectively. Expression rates of FHIT and PTEN in normal brain tissues were 80 % (4/ 5) . There was a statistically significant difference in the expression of FHIT and PTEN between normal brain tissues and different grades of gliomas ( P < 0. 05) . Both of the expressions of FHIT and PTEN weakened with the rise in the degree of glioma malignancy. Conclusion Expressions of FHIT and PTEN in glioma cells are diminishing or absent, their cooperative operation promotes generation and advance of glioma.
2007, 34(06): 425-427.
DOI: 10.3971/j.issn.1000-8578.1307
Abstract：
Objective To study the effect of natural killer (NK) cells on tumor growth inhibition of human nasopharyngeal carcinoma cell (CNE2 ) xenograft in BALB/c nude mice. Methods HLA typing of CNE2 cells and KIR (killer cell immunoglobulin-like receptor) genotypes were determined by PCR-SSP. Cytotoxicity of NK cells (isolated f rom 3 healthy persons ) against CNE2 cells were detected by LDH releasing assay. 12 BALB/c nude mice were divided into two groups ( the control group and the treatment group), 6 BALB/c nude mice in the treatment group were injected subcutaneously 1 ×106 CNE2 cells together with 3 ×10 NK cells were injected intravenously into the tail veins, while the cont rol group were just injected 1 ×106 CNE2 cells subcutaneously. The changes of tumor volume were observed, and the tumor growth inhibition was calculated. Results The HLA genotypes of CNE2 cells were A2, 24, B18, 35, Cw4, 7. KIR genotypes of the 3 healthy persons were KIR2DL1, KIR2DL3, KIR3DL1, KIR3DL2.Cytolysis of NK cells against CNE2 cells in vit ro were (9. 37 ±2. 14) %, (27. 14 ±1. 82) %, (36. 40 ±4. 28) % and (54. 67 ±2. 80) % respectively at 5∶1, 10∶1, 20∶1, 30∶1 E: T ratios. NK cells significantly inhibited the tumor growth in vivo, in the cont rol and treatment group, the rate of tumor formation were100 %(6/ 6 ), 83. 33 % ( 5/ 6 ), respectively ; the time of tumor formation were ( 18. 80 ±1. 64 ) d, (10. 00 ±2. 68) d, ( P < 0. 01) ; the average weight of tumors were (2. 22 ±0. 09) g, (1. 42 ±0. 09) g, ( P< 0. 01), respectively, The growth inhibitory rate was 36. 04 %. HE stain show that there were more necrosis regions and keratinization of tumor cells in the treatment group than that in the control group. Conclusion Our findings suggest that NK cells can inhibit the human nasopharyngeal carcinoma xenografts in nude mice and may become a novel treatment approach for human nasopharyngeal carcinoma.
Objective To study the effect of natural killer (NK) cells on tumor growth inhibition of human nasopharyngeal carcinoma cell (CNE2 ) xenograft in BALB/c nude mice. Methods HLA typing of CNE2 cells and KIR (killer cell immunoglobulin-like receptor) genotypes were determined by PCR-SSP. Cytotoxicity of NK cells (isolated f rom 3 healthy persons ) against CNE2 cells were detected by LDH releasing assay. 12 BALB/c nude mice were divided into two groups ( the control group and the treatment group), 6 BALB/c nude mice in the treatment group were injected subcutaneously 1 ×106 CNE2 cells together with 3 ×10 NK cells were injected intravenously into the tail veins, while the cont rol group were just injected 1 ×106 CNE2 cells subcutaneously. The changes of tumor volume were observed, and the tumor growth inhibition was calculated. Results The HLA genotypes of CNE2 cells were A2, 24, B18, 35, Cw4, 7. KIR genotypes of the 3 healthy persons were KIR2DL1, KIR2DL3, KIR3DL1, KIR3DL2.Cytolysis of NK cells against CNE2 cells in vit ro were (9. 37 ±2. 14) %, (27. 14 ±1. 82) %, (36. 40 ±4. 28) % and (54. 67 ±2. 80) % respectively at 5∶1, 10∶1, 20∶1, 30∶1 E: T ratios. NK cells significantly inhibited the tumor growth in vivo, in the cont rol and treatment group, the rate of tumor formation were100 %(6/ 6 ), 83. 33 % ( 5/ 6 ), respectively ; the time of tumor formation were ( 18. 80 ±1. 64 ) d, (10. 00 ±2. 68) d, ( P < 0. 01) ; the average weight of tumors were (2. 22 ±0. 09) g, (1. 42 ±0. 09) g, ( P< 0. 01), respectively, The growth inhibitory rate was 36. 04 %. HE stain show that there were more necrosis regions and keratinization of tumor cells in the treatment group than that in the control group. Conclusion Our findings suggest that NK cells can inhibit the human nasopharyngeal carcinoma xenografts in nude mice and may become a novel treatment approach for human nasopharyngeal carcinoma.
2007, 34(06): 428-431.
DOI: 10.3971/j.issn.1000-8578.694
Abstract：
Objective To test the expression of 5-lipoxygenase and 15-lipoxygenase-1 (15-LOX-1) mRNA and protein in gast ric carcinoma and adjacent normal tissue, and to investigate their relationship. Methods The 5-LOX and 15-LOX-1 mRNA and protein level were detected in pairs of gastric carcinoma and adjacent normal tissue by reverse transcription polymerase chain reaction (RT-PCR) and western blot methods. Results In gastric carcinoma, the expression of 5-LOX mRNA and protein was significantly higher than that in the adjacent normal tissue ( P < 0. 05), while the expression of 15-LOX-1 in gastric carcinoma was significantly lower than that in the adjacent normal tissue ( P < 0. 05) . No significant correlation was found between 5-LOX and 15-LOX-1 ( P > 0. 05) . Conclusion In gastric carcinoma, the expression of 5-LOX and 15-LOX-1 lost their balance, which was possibly correlated to the genesis and progress of gastric carcinoma. There was with close correlation between them.
Objective To test the expression of 5-lipoxygenase and 15-lipoxygenase-1 (15-LOX-1) mRNA and protein in gast ric carcinoma and adjacent normal tissue, and to investigate their relationship. Methods The 5-LOX and 15-LOX-1 mRNA and protein level were detected in pairs of gastric carcinoma and adjacent normal tissue by reverse transcription polymerase chain reaction (RT-PCR) and western blot methods. Results In gastric carcinoma, the expression of 5-LOX mRNA and protein was significantly higher than that in the adjacent normal tissue ( P < 0. 05), while the expression of 15-LOX-1 in gastric carcinoma was significantly lower than that in the adjacent normal tissue ( P < 0. 05) . No significant correlation was found between 5-LOX and 15-LOX-1 ( P > 0. 05) . Conclusion In gastric carcinoma, the expression of 5-LOX and 15-LOX-1 lost their balance, which was possibly correlated to the genesis and progress of gastric carcinoma. There was with close correlation between them.
2007, 34(06): 432-434.
DOI: 10.3971/j.issn.1000-8578.595
Abstract：
Objective To compare the infilt ration in melanoma models and cytotoxicity of natural killer cells activated by dendritic cells, IL-2 and IL-15. Methods Inoculating B16 Melanoma cells in C57BL/ 6 mice's back to establish subcutaneous models. NK cells labled by DAPI were injected via the lateral tail vein. DC, IL-2 and IL-15 were given respectively. Tumor tissues were removed at different times after injection and processed for electron microscope and fluorescence microscope. Cytotoxicity was detected by MTT assay. Results Cytotoxicity of NK cells cultured with DCs was enhanced. NK cells had higher cytotoxicity when N K/ DC ratio was 1∶1 than when it was 10∶1 ( P < 0. 05) . N K cells and DCs were identified through electron microscope and necrosis of target cells was observed. Infilt ration of NK cells was dose and time-dependent . More NK cells accumulated in tumors but not significantly when DCs were given compared to IL-2 or IL-15. Conclusion NK cells can be activated by DCs without exogenous cytotines. The activation between NK cells and DCs were related to their dose ratio.
Objective To compare the infilt ration in melanoma models and cytotoxicity of natural killer cells activated by dendritic cells, IL-2 and IL-15. Methods Inoculating B16 Melanoma cells in C57BL/ 6 mice's back to establish subcutaneous models. NK cells labled by DAPI were injected via the lateral tail vein. DC, IL-2 and IL-15 were given respectively. Tumor tissues were removed at different times after injection and processed for electron microscope and fluorescence microscope. Cytotoxicity was detected by MTT assay. Results Cytotoxicity of NK cells cultured with DCs was enhanced. NK cells had higher cytotoxicity when N K/ DC ratio was 1∶1 than when it was 10∶1 ( P < 0. 05) . N K cells and DCs were identified through electron microscope and necrosis of target cells was observed. Infilt ration of NK cells was dose and time-dependent . More NK cells accumulated in tumors but not significantly when DCs were given compared to IL-2 or IL-15. Conclusion NK cells can be activated by DCs without exogenous cytotines. The activation between NK cells and DCs were related to their dose ratio.
2007, 34(06): 435-438.
DOI: 10.3971/j.issn.1000-8578.701
Abstract：
Objective To observe the effect s of heparin on mouse hepatocarcinoma cell line Hca-F and Hca-P's adhesion in vitro and the lymphogenous metastasis in vivo. In order to detect how heparin plays its role. Methods Using Stamper-Woodruff method, cell chemistry and flow cytometry analysis to detect the effects of heparin in different concentrations. Results Heparin can inhibit obviously the adhesion between Hca-F and Hca-P cells and ice frozen sections of mouse lymph nodes in vitro, compared with saline-treatment group ( P < 0. 01) ; heparin can inhibit the lymphogenous metastasis of Hca-F cells in vivo, which has statistical significance between 100 U heparin group and saline-t reatment group ( P < 0. 05) . Conclusion Both Hca-F and Hca-P cells have the expression of L-selectin, but their expression level is different . The expression level of L-selectin on mouse hepatocarcinoma cell line Hca2F and Hca2P is positively related to the lymph node metastasis potential. Interaction between L-selectin and its ligand is inhibited partially by heparin. Therefore heparin have the competence of inhibiting tumor cell′s lymphogenous metastasis. Meanwhile, it also demonst rates that L-selectin can facilitate metastasis of Hca-F and Hca-P cells to the lymph node.
Objective To observe the effect s of heparin on mouse hepatocarcinoma cell line Hca-F and Hca-P's adhesion in vitro and the lymphogenous metastasis in vivo. In order to detect how heparin plays its role. Methods Using Stamper-Woodruff method, cell chemistry and flow cytometry analysis to detect the effects of heparin in different concentrations. Results Heparin can inhibit obviously the adhesion between Hca-F and Hca-P cells and ice frozen sections of mouse lymph nodes in vitro, compared with saline-treatment group ( P < 0. 01) ; heparin can inhibit the lymphogenous metastasis of Hca-F cells in vivo, which has statistical significance between 100 U heparin group and saline-t reatment group ( P < 0. 05) . Conclusion Both Hca-F and Hca-P cells have the expression of L-selectin, but their expression level is different . The expression level of L-selectin on mouse hepatocarcinoma cell line Hca2F and Hca2P is positively related to the lymph node metastasis potential. Interaction between L-selectin and its ligand is inhibited partially by heparin. Therefore heparin have the competence of inhibiting tumor cell′s lymphogenous metastasis. Meanwhile, it also demonst rates that L-selectin can facilitate metastasis of Hca-F and Hca-P cells to the lymph node.
2007, 34(06): 439-441.
DOI: 10.3971/j.issn.1000-8578.571
Abstract：
Objective To study the expression of DSB repair protein ( including Ku80, DNA-PKcs and ATM) in cervical carcinoma and cervical intra-epithelial neoplasia (CIN), and to explore their roles in neoplasia and tumor progression of cervical carcinoma. Methods Immunohistochemistry was used to detect the expressions of Ku80, DNA-PKcs, and ATM in 41 cases of cervical carcinoma and 15 cases of CIN. Results The positive percentages of Ku80, DNA-PKcs and ATM were 70. 7 %, 68. 3 % and 19. 5 % respectively in cervical carcinoma, and were 80. 0 %, 73. 3 % and 33. 3 % respectively in CIN ; the expression of three proteins in CIN were all higher than that in cervical carcinoma, but the differences has no statistical significance ( P > 0. 05) . The expression of these proteins was not associated with the age of patients, pathology, differentiation, and clinical stage ( P > 0. 05) . In all 56 cases, Ku80 was positively correlated with DNA2PKcs ( r = 0. 583, P = 0. 000) and ATM ( r = 0. 274, P = 0. 041) . Conclusion Three proteins might not play the major role in the neoplasia and tumor progression of cervical carcinoma ; Ku80 had closely relationship with DNA2PKcs and ATM in cervical carcinoma and CIN.
Objective To study the expression of DSB repair protein ( including Ku80, DNA-PKcs and ATM) in cervical carcinoma and cervical intra-epithelial neoplasia (CIN), and to explore their roles in neoplasia and tumor progression of cervical carcinoma. Methods Immunohistochemistry was used to detect the expressions of Ku80, DNA-PKcs, and ATM in 41 cases of cervical carcinoma and 15 cases of CIN. Results The positive percentages of Ku80, DNA-PKcs and ATM were 70. 7 %, 68. 3 % and 19. 5 % respectively in cervical carcinoma, and were 80. 0 %, 73. 3 % and 33. 3 % respectively in CIN ; the expression of three proteins in CIN were all higher than that in cervical carcinoma, but the differences has no statistical significance ( P > 0. 05) . The expression of these proteins was not associated with the age of patients, pathology, differentiation, and clinical stage ( P > 0. 05) . In all 56 cases, Ku80 was positively correlated with DNA2PKcs ( r = 0. 583, P = 0. 000) and ATM ( r = 0. 274, P = 0. 041) . Conclusion Three proteins might not play the major role in the neoplasia and tumor progression of cervical carcinoma ; Ku80 had closely relationship with DNA2PKcs and ATM in cervical carcinoma and CIN.
2007, 34(06): 442-445.
DOI: 10.3971/j.issn.1000-8578.682
Abstract：
Objective To explore the relationship s between VEGF, MMP-7 expression and progress, prognosis of colorectal Carcinoma. Methods SP immunohistochemical staining was used to examine expressions of VEGF, MMP-7 among 81 cases of colorectal carcinoma paraffin2embedded specimens. Rank sum test was used to analyze the expressions of VEGF, MMP-7. Results The expression of VEGF in colorectal carcinoma was positively correlated with depth of invasion ( P < 0. 01), lymph node metastasis ( P < 0. 01), distant metastasis ( P < 0. 01), Dukes stage. MMP-7 expression in colorectal carcinoma was also positively correlated with lymph node metastasis ( P < 0. 05), distant metastasis ( P < 0. 01), Dukes'stage. Expression of MMP-7 in mucous group was higher than that in high differentiation group ( P <0. 01) . The MMP-7 expression in patient s aged below 60y was st ronger than that in over 60y and 60y ( P< 0. 05) . Positive relationship appeared between VEGE and MMP-7. Conclusion VEGF, MMP-7 may improve the growth and metastasis of colorectal carcinoma. They could be prognostic factor of colorectal carcinoma. Decreasing the expression and activity of VEGF and MMP-7 might be a new pathway for the treatment of colorectal carcinoma.
Objective To explore the relationship s between VEGF, MMP-7 expression and progress, prognosis of colorectal Carcinoma. Methods SP immunohistochemical staining was used to examine expressions of VEGF, MMP-7 among 81 cases of colorectal carcinoma paraffin2embedded specimens. Rank sum test was used to analyze the expressions of VEGF, MMP-7. Results The expression of VEGF in colorectal carcinoma was positively correlated with depth of invasion ( P < 0. 01), lymph node metastasis ( P < 0. 01), distant metastasis ( P < 0. 01), Dukes stage. MMP-7 expression in colorectal carcinoma was also positively correlated with lymph node metastasis ( P < 0. 05), distant metastasis ( P < 0. 01), Dukes'stage. Expression of MMP-7 in mucous group was higher than that in high differentiation group ( P <0. 01) . The MMP-7 expression in patient s aged below 60y was st ronger than that in over 60y and 60y ( P< 0. 05) . Positive relationship appeared between VEGE and MMP-7. Conclusion VEGF, MMP-7 may improve the growth and metastasis of colorectal carcinoma. They could be prognostic factor of colorectal carcinoma. Decreasing the expression and activity of VEGF and MMP-7 might be a new pathway for the treatment of colorectal carcinoma.
2007, 34(06): 446-448.
DOI: 10.3971/j.issn.1000-8578.1397
Abstract：
Objective To study the correlation between the multislice helical CT(MSCT) feature of tumor blood supply type in patients with hepatocellular carcinoma ( HCC) and the serum level of vascular endothelial growth factor (VEGF) . Methods According to the cont rast-enhanced characteristic of lesion on MSCT, the tumor blood supply in 60 patients with HCC were classified into different types such as arterial blood supply, portal blood supply, arteroportal blood supply, and poorly blood supply. Enzyme linked immunosorbent assay ( ELISA) was used to test the serum levels of VEGF collected from 20 healthy individuals and 60 patient s with HCC respectively. At last, the CT features of tumor blood supply type were performed statistical analysis with the serum levels of VEGF. Results The serum levels of VEGF in 20 patients with arterial blood supply type and 20 patients with arteroportal blood supply were significantly higher than that in 20 healthy individuals and 20 patients with portal blood supply or poorly blood supply type ( P < 0. 01) . There was no statistical significance of serum VEGF levels between 20 patients with portal blood supply or poorly blood supply type and 20 healthy individuals ( P > 0. 05) . Conclusion The pattern of tumor blood supply for HCC on MSCT is correlative with the serum level of VEGF, and it is of great value in the interventional therapy for HCC.
Objective To study the correlation between the multislice helical CT(MSCT) feature of tumor blood supply type in patients with hepatocellular carcinoma ( HCC) and the serum level of vascular endothelial growth factor (VEGF) . Methods According to the cont rast-enhanced characteristic of lesion on MSCT, the tumor blood supply in 60 patients with HCC were classified into different types such as arterial blood supply, portal blood supply, arteroportal blood supply, and poorly blood supply. Enzyme linked immunosorbent assay ( ELISA) was used to test the serum levels of VEGF collected from 20 healthy individuals and 60 patient s with HCC respectively. At last, the CT features of tumor blood supply type were performed statistical analysis with the serum levels of VEGF. Results The serum levels of VEGF in 20 patients with arterial blood supply type and 20 patients with arteroportal blood supply were significantly higher than that in 20 healthy individuals and 20 patients with portal blood supply or poorly blood supply type ( P < 0. 01) . There was no statistical significance of serum VEGF levels between 20 patients with portal blood supply or poorly blood supply type and 20 healthy individuals ( P > 0. 05) . Conclusion The pattern of tumor blood supply for HCC on MSCT is correlative with the serum level of VEGF, and it is of great value in the interventional therapy for HCC.
2007, 34(06): 449-450.
DOI: 10.3971/j.issn.1000-8578.573
Abstract：
Objective To investigate the abnormality of configuration of microvessel in hepatocellular carcinoma ( HCC) . Methods Expressions of CD34 and Endoglin were studied through immunohistochemistry in fifty-six cases of HCC and their paraneoplastic liver tissue and six cases of normal liver tissue. The ultramicrost ructure of microvessel was observed using transmission elect ron microscope. Results Microvessel density (MVD) denoted by CD34 and Endoglin were 127. 1 ±5. 9 and 64. 6 ±11. 1 respectively in HCC, cont rasted with 7. 1 ±1. 3 and 6. 1 ±1. 2 in paraneoplastic liver tissue ( P < 0. 01) . The structure of microvessel was loose and the permeability increased in HCC. Microthrombi formed in the microvessel in HCC. Conclusion There was a large amount of st ructurally defect microvessel in HCC, which provided route for cancer metastasis.
Objective To investigate the abnormality of configuration of microvessel in hepatocellular carcinoma ( HCC) . Methods Expressions of CD34 and Endoglin were studied through immunohistochemistry in fifty-six cases of HCC and their paraneoplastic liver tissue and six cases of normal liver tissue. The ultramicrost ructure of microvessel was observed using transmission elect ron microscope. Results Microvessel density (MVD) denoted by CD34 and Endoglin were 127. 1 ±5. 9 and 64. 6 ±11. 1 respectively in HCC, cont rasted with 7. 1 ±1. 3 and 6. 1 ±1. 2 in paraneoplastic liver tissue ( P < 0. 01) . The structure of microvessel was loose and the permeability increased in HCC. Microthrombi formed in the microvessel in HCC. Conclusion There was a large amount of st ructurally defect microvessel in HCC, which provided route for cancer metastasis.
2007, 34(06): 451-453.
DOI: 10.3971/j.issn.1000-8578.2806
Abstract：
Objective To evaluate the effects of epoetin alfa ( EPO) therapy on hemoglobin ( Hb), transfusion requirements and quality of life (QOL) in patient s with cancer receiving chemotherapy. Methods Seventy-nine patient s with Hb ≤12. 0g/ dL were randomized to receive EPO 8 000U three times per week s. c ( EPO group) or best supportive care (BSC group) for 8 weeks. Hemoglobin responses, transfusion requirements, QOL and toxicity were measured. Results EPO maintained Hb levels throughout the study with mean Hb levels ≥12. 0 g/ dL, compared with a decrease with BSC. Hemoglobin responses were 53. 8 %for EPO group, significantly higher than that in BSC group (7. 5 %) ( P < 0. 0001) . Transfusion requirement s in EPO patient s and BSC patients were 7. 7 % and 30 % (χ2 = 6. 388, P < 0. 05), respectively. At the eighth week, mean change score of FACT-An anemia and fatigue was (2. 16 ±12. 84), (3. 58 ±10. 52) for EPO group and ( - 4. 43 ±13. 42), ( - 5. 34 ±11. 14) for BSC group ( P < 0. 0001 for both comparisons) . Adverse event s were similar between the groups. Conclusion EPO maintains hemoglobin level, reduces t ransfusion requirements and improves QOL in patient s with cancer receiving chemotherapy.
Objective To evaluate the effects of epoetin alfa ( EPO) therapy on hemoglobin ( Hb), transfusion requirements and quality of life (QOL) in patient s with cancer receiving chemotherapy. Methods Seventy-nine patient s with Hb ≤12. 0g/ dL were randomized to receive EPO 8 000U three times per week s. c ( EPO group) or best supportive care (BSC group) for 8 weeks. Hemoglobin responses, transfusion requirements, QOL and toxicity were measured. Results EPO maintained Hb levels throughout the study with mean Hb levels ≥12. 0 g/ dL, compared with a decrease with BSC. Hemoglobin responses were 53. 8 %for EPO group, significantly higher than that in BSC group (7. 5 %) ( P < 0. 0001) . Transfusion requirement s in EPO patient s and BSC patients were 7. 7 % and 30 % (χ2 = 6. 388, P < 0. 05), respectively. At the eighth week, mean change score of FACT-An anemia and fatigue was (2. 16 ±12. 84), (3. 58 ±10. 52) for EPO group and ( - 4. 43 ±13. 42), ( - 5. 34 ±11. 14) for BSC group ( P < 0. 0001 for both comparisons) . Adverse event s were similar between the groups. Conclusion EPO maintains hemoglobin level, reduces t ransfusion requirements and improves QOL in patient s with cancer receiving chemotherapy.
2007, 34(06): 454-456.
DOI: 10.3971/j.issn.1000-8578.577
Abstract：
Objective To investigate clinical characteristics and risk factors of Candida species infection in patients with malignant tumors for the guidance of the prevention and surveillance of Candida infection. Methods A ret rospective clinical study was performed in Tangshan people's hospital for 24 months (January 2004 to December 2005) . In 785 patients with malignant tumor, clinical records of 153 patients with Candida infection were collected. Results Candida infection rate was 21. 02 %. The major Candida species was Candida albicans (77. 78 %), the second was Candida glabrata (11. 77 %) . The main locations of infection were mouth cavity and upper respiratory t ract (55. 75 %) . Sex had no influence on Candida infection. The main risk factors were age ≥60 years, long-term hospitalzation, cancer stage, chemotherapy and radiotherapy, comprehensive application of antibiotics and hormones, diverse aggressive techniques of diagnosis and treatment . Conclusion Candida species is a major opportunistic pathogen. Candida infection was the result of advances in medical treatment and increasing numbers of immunocompromised patients with cancer. The prevention and surveillance of Candida infection must be emphasized.
Objective To investigate clinical characteristics and risk factors of Candida species infection in patients with malignant tumors for the guidance of the prevention and surveillance of Candida infection. Methods A ret rospective clinical study was performed in Tangshan people's hospital for 24 months (January 2004 to December 2005) . In 785 patients with malignant tumor, clinical records of 153 patients with Candida infection were collected. Results Candida infection rate was 21. 02 %. The major Candida species was Candida albicans (77. 78 %), the second was Candida glabrata (11. 77 %) . The main locations of infection were mouth cavity and upper respiratory t ract (55. 75 %) . Sex had no influence on Candida infection. The main risk factors were age ≥60 years, long-term hospitalzation, cancer stage, chemotherapy and radiotherapy, comprehensive application of antibiotics and hormones, diverse aggressive techniques of diagnosis and treatment . Conclusion Candida species is a major opportunistic pathogen. Candida infection was the result of advances in medical treatment and increasing numbers of immunocompromised patients with cancer. The prevention and surveillance of Candida infection must be emphasized.
2007, 34(06): 457-458.
DOI: 10.3971/j.issn.1000-8578.579
Abstract：
Objective To observe the response and adverse reactions of Durogesic ( Transdermal Fentanyl) in the t reatment of cancer patients with moderate or severe pain. Methods Sixty advanced cancer patients with moderate or severe cancer pain were treated with Durogesic. evaluated the pain relief degree according to Numerical Rating Scale (NRS) and investigated the quality of life by the score criterion of Quality of Life (QOL), and observed the adverse reactions. Results The pain remission rate in cancer patient s of this group was 98. 3 %, including excellent remission rate 41. 7 % and effective remission rate 51. 67 %.The main adverse actions were nausea and vomiting, constipation, and dizziness. Conclusion Durogesic has significant efficacy, and mild adverse reactions, it is a simple way to using Durogesic for relieving moderate or severe cancer pain. Durogesic can improve the quality of life markedly.
Objective To observe the response and adverse reactions of Durogesic ( Transdermal Fentanyl) in the t reatment of cancer patients with moderate or severe pain. Methods Sixty advanced cancer patients with moderate or severe cancer pain were treated with Durogesic. evaluated the pain relief degree according to Numerical Rating Scale (NRS) and investigated the quality of life by the score criterion of Quality of Life (QOL), and observed the adverse reactions. Results The pain remission rate in cancer patient s of this group was 98. 3 %, including excellent remission rate 41. 7 % and effective remission rate 51. 67 %.The main adverse actions were nausea and vomiting, constipation, and dizziness. Conclusion Durogesic has significant efficacy, and mild adverse reactions, it is a simple way to using Durogesic for relieving moderate or severe cancer pain. Durogesic can improve the quality of life markedly.
2007, 34(06): 459-461.
DOI: 10.3971/j.issn.1000-8578.2701
Abstract：
Objective To generalize the experience in the diagnosis and t reatment of invasive thymoma. Methods By performing retrospective study on 58 patients, to generalize the experience in the diagnosis and treatment of such disease. Results In the aspect of operative approaches, 58 patients were divided into two groups, namely median sternotomy (43 cases) and anterolateral approach (15 cases) . 41 cases received complete tumor resection, 14 cases received palliative resection and others just received tumor biopsy. Among those with complete thymoma resection, 36 cases were performed enlarged tumor resection, including mediastinal pleural resection (27 cases), lung wedge resection (7 cases), superior vena cava or innominate vein partial resection and vascularplasty (9 cases) and replacement of superior vena cava (4 cases) . seventeen patient s with Ⅲ and Ⅳ stage thymoma only received palliative resection or tumor biopsy. The residual tumor tissues were implanted radioactive particles (125I) in 14 patient s and 1 patient received radiof requency hyperthermia. All the patients survived and those, received complete tumor resection, had recovered very well and had higher quality of life, except only 1 case had still edema symptom in his face after replacement of superior vena cava. Among 58 patients, 23 patients survived 3 years and the survival rate was 39. 7 %. Conclusion Surgical therapy is playing an important role in the treatment of invasive thymoma. Radical tumor resection is very necessary for those suitable for operation and precautions must be taken to remove all the mediastinal fat tissues in order to reduce thymoma recurrence rate. For those with so large thymomas that can not be resected completely, in order to improving resection rate and prolonging survival time, therapy plan should add radiotherapy and (or) chemotherapy before (or after) operation.
Objective To generalize the experience in the diagnosis and t reatment of invasive thymoma. Methods By performing retrospective study on 58 patients, to generalize the experience in the diagnosis and treatment of such disease. Results In the aspect of operative approaches, 58 patients were divided into two groups, namely median sternotomy (43 cases) and anterolateral approach (15 cases) . 41 cases received complete tumor resection, 14 cases received palliative resection and others just received tumor biopsy. Among those with complete thymoma resection, 36 cases were performed enlarged tumor resection, including mediastinal pleural resection (27 cases), lung wedge resection (7 cases), superior vena cava or innominate vein partial resection and vascularplasty (9 cases) and replacement of superior vena cava (4 cases) . seventeen patient s with Ⅲ and Ⅳ stage thymoma only received palliative resection or tumor biopsy. The residual tumor tissues were implanted radioactive particles (125I) in 14 patient s and 1 patient received radiof requency hyperthermia. All the patients survived and those, received complete tumor resection, had recovered very well and had higher quality of life, except only 1 case had still edema symptom in his face after replacement of superior vena cava. Among 58 patients, 23 patients survived 3 years and the survival rate was 39. 7 %. Conclusion Surgical therapy is playing an important role in the treatment of invasive thymoma. Radical tumor resection is very necessary for those suitable for operation and precautions must be taken to remove all the mediastinal fat tissues in order to reduce thymoma recurrence rate. For those with so large thymomas that can not be resected completely, in order to improving resection rate and prolonging survival time, therapy plan should add radiotherapy and (or) chemotherapy before (or after) operation.
