2007 Vol. 34 No. 02
2007, 34(02): 83-85.
DOI: 10.3971/j.issn.1000-8578.3251
Abstract：
Objective To investigate antitumor effects induced by dendritic cells (DCs) pulsed with complex of tumor antigen from elemene-combo tumor cell vaccine-heat shock protein 70 of BCG ( HTA-HSP70BCG) . Methods Tumor antigen peptides from elemene-combo Hca-F cell ( HTA ) combined with HSP70BCGinto HTA-HSP70BCG in vitro. DCs were induced in medium with GM-CSF and IL-4 and pulsed with HTA-HSP70BCG, HTA and HSP70BCG, respectively. The proliferation stimulating effects on un-separated splenocytes and the cytotoxicity of the splenocytes activated with DCs were evaluated with MTT assay. The expression of CD40 and CD86 on the surface of DCs was assessed by flow cytometer. Results Pulsing of HTA-HSP70BCG, resulted in DCs maturation, characterized by up-regulation of CD86 and CD40. Proliferation index of un-separated splenocytes from HTA-HSP70BCG group was significantly increased as compared with HTA group . Un-separated splenocytes from DCs pulsed with HTA-HSP70BCG revealed the cytotoxicity agaist Hca-F, The HTA failed to reveal the cytotoxicity against Hca-F. Conclusion HTA-HSP70BCG could induce DCs maturation and the mature DCs could activate splenocytes to generate more potent specific antitumor effect .
Objective To investigate antitumor effects induced by dendritic cells (DCs) pulsed with complex of tumor antigen from elemene-combo tumor cell vaccine-heat shock protein 70 of BCG ( HTA-HSP70BCG) . Methods Tumor antigen peptides from elemene-combo Hca-F cell ( HTA ) combined with HSP70BCGinto HTA-HSP70BCG in vitro. DCs were induced in medium with GM-CSF and IL-4 and pulsed with HTA-HSP70BCG, HTA and HSP70BCG, respectively. The proliferation stimulating effects on un-separated splenocytes and the cytotoxicity of the splenocytes activated with DCs were evaluated with MTT assay. The expression of CD40 and CD86 on the surface of DCs was assessed by flow cytometer. Results Pulsing of HTA-HSP70BCG, resulted in DCs maturation, characterized by up-regulation of CD86 and CD40. Proliferation index of un-separated splenocytes from HTA-HSP70BCG group was significantly increased as compared with HTA group . Un-separated splenocytes from DCs pulsed with HTA-HSP70BCG revealed the cytotoxicity agaist Hca-F, The HTA failed to reveal the cytotoxicity against Hca-F. Conclusion HTA-HSP70BCG could induce DCs maturation and the mature DCs could activate splenocytes to generate more potent specific antitumor effect .
2007, 34(02): 86-88.
DOI: 10.3971/j.issn.1000-8578.3245
Abstract：
Objective To construct and identify the siRNA eukaryotic expression vector targeting PEG10. Methods Four siRNAs were designed according to the coding sequence of PEG10 gene, and cloned into the downst ream of H1 promoter of psiRNA-hH1neo. The const ructed recombinant was analyzed and identified by Ase Ⅰendonuclease digestion and DNA sequencing. Results The constructed psiRNA plasmid digested with Ase Ⅰwas linearized. The sequencing result confirmed that the sequence of inserted fragment was correct . Conclusion Eukaryotic expression vector of siRNA targeting PEG10 gene was successfully constructed, and should be a novel effective expression vector for HCC gene therapy.
Objective To construct and identify the siRNA eukaryotic expression vector targeting PEG10. Methods Four siRNAs were designed according to the coding sequence of PEG10 gene, and cloned into the downst ream of H1 promoter of psiRNA-hH1neo. The const ructed recombinant was analyzed and identified by Ase Ⅰendonuclease digestion and DNA sequencing. Results The constructed psiRNA plasmid digested with Ase Ⅰwas linearized. The sequencing result confirmed that the sequence of inserted fragment was correct . Conclusion Eukaryotic expression vector of siRNA targeting PEG10 gene was successfully constructed, and should be a novel effective expression vector for HCC gene therapy.
2007, 34(02): 88-92.
DOI: 10.3971/j.issn.1000-8578.3237
Abstract：
Objective To study effects of inducible nitric oxide synthase (iNOS)-derived nitric oxide (NO) on apoptosis of HL60 cells co2cultured with RAW 264. 7 macrophages. Methods Upon stimulation with lipopolysaccharide (LPS) and interferon-γ( IFN-γ), inducible nitric oxide synthase gene was expressed in RAW 264. 7 macrophages, which caused the consequent generation of nitric oxide. Effects of nitric oxide on HL60 cells viability, expression of bcl-2 and bax protein, activity of Caspase-3 and cell apoptosis were evaluated with MTT assay, Western blot analysis, fluorescence analysis, flow cytomet ry (FCM), transmission elect ron microscopy ( TEM) and DNA agarose gelelect rophoresis. Results The results showed that iNOS-derived nitric oxide caused oxidative damage of HL60 cells co-cultured with RAW 264. 7 macrophages, and decreased cell viability, and evidently reduced expression of bcl-2 and increased expression of bax, and induced activity of caspase-3 and DNA f ragmentation. Conclusion The result s suggested important effect of iNOS-derived nitric oxide on apoptosis of cells in RAW 264. 7 macrophages.
Objective To study effects of inducible nitric oxide synthase (iNOS)-derived nitric oxide (NO) on apoptosis of HL60 cells co2cultured with RAW 264. 7 macrophages. Methods Upon stimulation with lipopolysaccharide (LPS) and interferon-γ( IFN-γ), inducible nitric oxide synthase gene was expressed in RAW 264. 7 macrophages, which caused the consequent generation of nitric oxide. Effects of nitric oxide on HL60 cells viability, expression of bcl-2 and bax protein, activity of Caspase-3 and cell apoptosis were evaluated with MTT assay, Western blot analysis, fluorescence analysis, flow cytomet ry (FCM), transmission elect ron microscopy ( TEM) and DNA agarose gelelect rophoresis. Results The results showed that iNOS-derived nitric oxide caused oxidative damage of HL60 cells co-cultured with RAW 264. 7 macrophages, and decreased cell viability, and evidently reduced expression of bcl-2 and increased expression of bax, and induced activity of caspase-3 and DNA f ragmentation. Conclusion The result s suggested important effect of iNOS-derived nitric oxide on apoptosis of cells in RAW 264. 7 macrophages.
2007, 34(02): 93-95.
DOI: 10.3971/j.issn.1000-8578.3247
Abstract：
Objective Glial cells play essential roles in creation and maintenance of pain state. This study was designed to explore the activation of glial cells in vincristine-induced neuropathic pain, and to find how glial cells influence pain threshold. Methods We adapted a model by using repeated int raperitoned injection of vincristine. By immunohistochchemical technique, the expression of specific activation markers of ast rocytes and microglials, glial fibrillary acidic protein ( GFAP) and OX-42 respectively, were examined in brain and lumbar intumescentia. Using RT-PCR analysis techniques, the expression of interleukine-1β( IL-1β) and glial cell line-derived neurot rophic factor ( GDNF) in lumbar intumescentia were tested. Results Mechanical hyperalgsia appeared on the 8th>>> day and thermal hyperalgsia appeared on the 5th>>> day of vincristine treatment . Glial cells were obviously active in periaquenductal gray and spinal gray.IL-1βexpression was increased in chemotherapy group, while GDNF was higher in control group. Conclusion Glial cells were active in Vincristine-induced neuropathic pain. The change of expression of IL-1βand GDNF were involved in neuropathic pain evoked by vincristine.
Objective Glial cells play essential roles in creation and maintenance of pain state. This study was designed to explore the activation of glial cells in vincristine-induced neuropathic pain, and to find how glial cells influence pain threshold. Methods We adapted a model by using repeated int raperitoned injection of vincristine. By immunohistochchemical technique, the expression of specific activation markers of ast rocytes and microglials, glial fibrillary acidic protein ( GFAP) and OX-42 respectively, were examined in brain and lumbar intumescentia. Using RT-PCR analysis techniques, the expression of interleukine-1β( IL-1β) and glial cell line-derived neurot rophic factor ( GDNF) in lumbar intumescentia were tested. Results Mechanical hyperalgsia appeared on the 8th>>> day and thermal hyperalgsia appeared on the 5th>>> day of vincristine treatment . Glial cells were obviously active in periaquenductal gray and spinal gray.IL-1βexpression was increased in chemotherapy group, while GDNF was higher in control group. Conclusion Glial cells were active in Vincristine-induced neuropathic pain. The change of expression of IL-1βand GDNF were involved in neuropathic pain evoked by vincristine.
2007, 34(02): 96-99.
DOI: 10.3971/j.issn.1000-8578.572
Abstract：
Objective To construct sense and antisense human bone sialoprotein (BSP) eukaryotic expression vector and observe influence of it s expression in human osteosarcoma cell line MG-63 cells about invasion and metastasis of osteosarcoma. Methods An open reading f rame of human BSP was amplified by PCR method and was reconst ructed into the eukaryotic expressive vector p IRES2-EGFP to construct the hBSP sense or antisense expressive vector . The sense and antisense hBSP eukaryotic expression vector was transfected into MG-63 cells with lipofec tamine. The expression of BSP on the cells was measured by Western blotting. Transwell-chamber methodand scarification was used to evaluate influence of BSP expression on osteosarcoma cell invasion and metastasis. Results The sense and antisense hBSP eukaryotic vector was successfully const ructed and t ransfected. Compared with cont rol cells, expression of BSP increased in the cells by sense hBSP eukaryotic vector transfected and invasive power of these cells is enhance, but expression of BSP decreased in the cells by antisense hBSP eukaryotic vector transfected and invasive power of these cells is been inhabited. Conclusion The change of BSP expression may play an important role in osteosarcoma metastasis. The down-regulation of BSP expression can inhibit abilities of osteosarcoma cell invasion and metastasis.
Objective To construct sense and antisense human bone sialoprotein (BSP) eukaryotic expression vector and observe influence of it s expression in human osteosarcoma cell line MG-63 cells about invasion and metastasis of osteosarcoma. Methods An open reading f rame of human BSP was amplified by PCR method and was reconst ructed into the eukaryotic expressive vector p IRES2-EGFP to construct the hBSP sense or antisense expressive vector . The sense and antisense hBSP eukaryotic expression vector was transfected into MG-63 cells with lipofec tamine. The expression of BSP on the cells was measured by Western blotting. Transwell-chamber methodand scarification was used to evaluate influence of BSP expression on osteosarcoma cell invasion and metastasis. Results The sense and antisense hBSP eukaryotic vector was successfully const ructed and t ransfected. Compared with cont rol cells, expression of BSP increased in the cells by sense hBSP eukaryotic vector transfected and invasive power of these cells is enhance, but expression of BSP decreased in the cells by antisense hBSP eukaryotic vector transfected and invasive power of these cells is been inhabited. Conclusion The change of BSP expression may play an important role in osteosarcoma metastasis. The down-regulation of BSP expression can inhibit abilities of osteosarcoma cell invasion and metastasis.
2007, 34(02): 100-102.
DOI: 10.3971/j.issn.1000-8578.574
Abstract：
Objective To explore expression of PTEN in oral squamous cell carcinoma (OSCC) and its clinicopathological significance. Methods PTEN protein expression in paraffin embedded tissues from 10 cases of normal oral mucosa, 10 cases of oral epithelial hyperplasia, 15 cases of oral leukoplakia and 72 cases of OSCC was analyzed by SP immunohistochemical method and tissue microarray techniques, meanwhile analysed clinical pathological data. Results The positive rate of PTEN protein was 72. 2 %(52/72) 、93. 3 %(14/15) 、100 %(10/10) and 100 %(10/10) in OSCC, oral leukoplakia, oral epithelial hyperplasia and normal oral mucosa, there was significant correlation among them( P < 0. 05) . PTEN expression was not obviously correlated with age, different gender, and TNM classification etc ( P > 0. 05), but there was significant correlation with nodal metastases and degree of OSCC ( P < 0. 05) . Conclusion Loss of PTEN protein expression may play an important role on the tumorigenesis and development of OSCC, PTEN can be used as a reference marker for judging prognosis of patient s with OSCC.
Objective To explore expression of PTEN in oral squamous cell carcinoma (OSCC) and its clinicopathological significance. Methods PTEN protein expression in paraffin embedded tissues from 10 cases of normal oral mucosa, 10 cases of oral epithelial hyperplasia, 15 cases of oral leukoplakia and 72 cases of OSCC was analyzed by SP immunohistochemical method and tissue microarray techniques, meanwhile analysed clinical pathological data. Results The positive rate of PTEN protein was 72. 2 %(52/72) 、93. 3 %(14/15) 、100 %(10/10) and 100 %(10/10) in OSCC, oral leukoplakia, oral epithelial hyperplasia and normal oral mucosa, there was significant correlation among them( P < 0. 05) . PTEN expression was not obviously correlated with age, different gender, and TNM classification etc ( P > 0. 05), but there was significant correlation with nodal metastases and degree of OSCC ( P < 0. 05) . Conclusion Loss of PTEN protein expression may play an important role on the tumorigenesis and development of OSCC, PTEN can be used as a reference marker for judging prognosis of patient s with OSCC.
2007, 34(02): 103-105.
DOI: 10.3971/j.issn.1000-8578.586
Abstract：
Objective To screen NS gene specific positive cell clones, and investigate the effect of human esophagus cancer Eca-109 cells proliferation in vit ro by NS gene specific RNA interference. Methods The NS-siRNA expression vector was transfected into human esophagus cancer Eca-109 cells using LIPOFECTAMINETM 2000 Reagent, and positive clones were examined by PCR af ter stable transfectans screening with Zeocin ; detected the cell proliferation by MTT assay and the levels of NS gene expression by RT-PCR. Results Compared with the two control groups, the silencer group cells were nearer differetiation, the proliferation inhibitory rate was higher than 80 %( P < 0. 01) ; the level of NS gene expression reduced. Conclusion The NS gene specific RNAi can significantly inhibit human esophagus cancer Eca-109 cells proliferation in vitro and reduce the level of NS gene expression.
Objective To screen NS gene specific positive cell clones, and investigate the effect of human esophagus cancer Eca-109 cells proliferation in vit ro by NS gene specific RNA interference. Methods The NS-siRNA expression vector was transfected into human esophagus cancer Eca-109 cells using LIPOFECTAMINETM 2000 Reagent, and positive clones were examined by PCR af ter stable transfectans screening with Zeocin ; detected the cell proliferation by MTT assay and the levels of NS gene expression by RT-PCR. Results Compared with the two control groups, the silencer group cells were nearer differetiation, the proliferation inhibitory rate was higher than 80 %( P < 0. 01) ; the level of NS gene expression reduced. Conclusion The NS gene specific RNAi can significantly inhibit human esophagus cancer Eca-109 cells proliferation in vitro and reduce the level of NS gene expression.
2007, 34(02): 106-108.
DOI: 10.3971/j.issn.1000-8578.575
Abstract：
Objective To evaluate the expressions of GST-π, LRP, Topo Ⅱa, MRP and MDR in 113 non-small cell lung cancer (NSCLC), to observe their relationships with clinicopathologic characterization of cancer and their interactions. Methods Expressions of GST-π, LRP and Topo Ⅱa were detected by S-P immunohistochemistry, and expressions of MRP, MDR1 mRNA in paraffin-embedded cancer tissues were detected by in situ hybridization. Results (1) The positive rates of GST-π, LRP, Topo Ⅱa and MRP, MDR1 mRNA were 75. 2 %, 80. 5 %, 60. 2 %, 79. 6 % and 51. 3 % in NSCLC, respectively. (2) The expressions of LRP, Topo Ⅱa, MRP had a close relationship with the histological types in NSCLC, respectively. (3) There were significant relationships between positive rates of GST-π and MRP ( P <0. 05), LRP and MRP( P < 0. 01), MDR1 and MRP ( P < 0. 01), respectively. Conclusion (1) The overexpressions and interactions of GST-π, LRP, Topo Ⅱa, MRP and MDR are important causes of primary MDR in NSCLC. (2) The overexpressions of LRP and MRP, the overexpression of Topo Ⅱa and the reduced expression of MRP, which are related with the chemotherapeutic sensitivity in adenocarcinoma and squamous carcinoma, respectively.
Objective To evaluate the expressions of GST-π, LRP, Topo Ⅱa, MRP and MDR in 113 non-small cell lung cancer (NSCLC), to observe their relationships with clinicopathologic characterization of cancer and their interactions. Methods Expressions of GST-π, LRP and Topo Ⅱa were detected by S-P immunohistochemistry, and expressions of MRP, MDR1 mRNA in paraffin-embedded cancer tissues were detected by in situ hybridization. Results (1) The positive rates of GST-π, LRP, Topo Ⅱa and MRP, MDR1 mRNA were 75. 2 %, 80. 5 %, 60. 2 %, 79. 6 % and 51. 3 % in NSCLC, respectively. (2) The expressions of LRP, Topo Ⅱa, MRP had a close relationship with the histological types in NSCLC, respectively. (3) There were significant relationships between positive rates of GST-π and MRP ( P <0. 05), LRP and MRP( P < 0. 01), MDR1 and MRP ( P < 0. 01), respectively. Conclusion (1) The overexpressions and interactions of GST-π, LRP, Topo Ⅱa, MRP and MDR are important causes of primary MDR in NSCLC. (2) The overexpressions of LRP and MRP, the overexpression of Topo Ⅱa and the reduced expression of MRP, which are related with the chemotherapeutic sensitivity in adenocarcinoma and squamous carcinoma, respectively.
2007, 34(02): 109-111.
DOI: 10.3971/j.issn.1000-8578.2409
Abstract：
Objective To investigate the expression of FHIT gene in lung carcinoma and its relationship with differentiation degree and histological types and the expression of p53 gene. Methods The expressions of FHIT and p53 were detected by immunohistochemistry method and tissue microarray technique in the specimens of 110 lung carcinoma and 25 benign lung lesions. Results ①Abnormal expression rate of FHIT was 16 % in benign lung lesions, 85. 5 % in lung carcinoma, the difference was very significant between them( P < 0. 01) ; when the differentiation degree was different, the difference of FHIT expression in the lung carcinoma was very significant ( P < 0. 01), and FHIT expression was positive correlation with differentiation degree ( r = 0. 608, P < 0. 01) ; when the histological types were different, the difference of FHIT expression in the lung carcinoma was not statistical significance ( P > 0. 05) ; ②comparing the negative expression group with the positive one of p53, the difference of FHIT expression in the lung carcinoma was not statistical significance ( P > 0. 05) ;in lung carcinoma abnormal expression rate of p53 was 53. 6 %, abnormal expression rate of FHIT was 85. 4 %, the difference was statistical significance ( P< 0. 01) ;the rate of union detection was 93. 6 %. Conclusion ①FHIT protein may become the molecular maker of earlier period diagnosing of lung carcinoma, and the union detection of FHIT, p53 protein will help to improve the detection rate ; ②FHIT protein influences direction of differentiation of the lung carcinoma cell, and this will offer new thinking for the development of clinical tumour drug.
Objective To investigate the expression of FHIT gene in lung carcinoma and its relationship with differentiation degree and histological types and the expression of p53 gene. Methods The expressions of FHIT and p53 were detected by immunohistochemistry method and tissue microarray technique in the specimens of 110 lung carcinoma and 25 benign lung lesions. Results ①Abnormal expression rate of FHIT was 16 % in benign lung lesions, 85. 5 % in lung carcinoma, the difference was very significant between them( P < 0. 01) ; when the differentiation degree was different, the difference of FHIT expression in the lung carcinoma was very significant ( P < 0. 01), and FHIT expression was positive correlation with differentiation degree ( r = 0. 608, P < 0. 01) ; when the histological types were different, the difference of FHIT expression in the lung carcinoma was not statistical significance ( P > 0. 05) ; ②comparing the negative expression group with the positive one of p53, the difference of FHIT expression in the lung carcinoma was not statistical significance ( P > 0. 05) ;in lung carcinoma abnormal expression rate of p53 was 53. 6 %, abnormal expression rate of FHIT was 85. 4 %, the difference was statistical significance ( P< 0. 01) ;the rate of union detection was 93. 6 %. Conclusion ①FHIT protein may become the molecular maker of earlier period diagnosing of lung carcinoma, and the union detection of FHIT, p53 protein will help to improve the detection rate ; ②FHIT protein influences direction of differentiation of the lung carcinoma cell, and this will offer new thinking for the development of clinical tumour drug.
2007, 34(02): 112-114.
DOI: 10.3971/j.issn.1000-8578.576
Abstract：
Objective To evaluate the expression of endostatin in bronchoalveolar lavage fluid (BALF) and serum in patients with lung cancer. And to analysis the relationship between the levels of it and clinical features as well as pathophysiological characteristics. Methods The samples of serum and BAL F were obtained from 47 patient s with untreated primary lung cancer and 18 patients with benign pulmonary diseases. The levels of endostatin were analyzed by enzyme-linked immunosorbent assay ( ELISA) . Results The expression of endostatin in serum was (131. 71 ±50. 32) ng/ ml and in BALF was (502. 56 ±302. 00)ng/ ml in patient s with lung cancer. And the expression of endostatin in patient s with benign pulmonary diseases was (85. 86 ±36. 86) ng/ ml and (279. 49 ±135. 17) ng/ ml, respectively ( P < 0. 01), there was significantly difference between them. The levels of endostatin in serum and BALF in advanced stage lung cancer patient s were higher than those of early stage patients. In adenocarcinoma patients was significantly higher than that in patients with squamous cell carcinoma and SCLC in serum and BAL F. Endostatin concent rations in BALF and in serum were significantly higher in patients with lymph node and distant metastasis than those patient s with no metastasis. The expression of endostatin in serum was closely related to that in BAL F in patients with lung cancer ( P = 0. 000) . Conclusion The detection of endostatin in serum and in BALF may be helpful to distinguish the lung cancer from benign pulmonary diseases and to reveal the biological behaviors of lung cancer well.
Objective To evaluate the expression of endostatin in bronchoalveolar lavage fluid (BALF) and serum in patients with lung cancer. And to analysis the relationship between the levels of it and clinical features as well as pathophysiological characteristics. Methods The samples of serum and BAL F were obtained from 47 patient s with untreated primary lung cancer and 18 patients with benign pulmonary diseases. The levels of endostatin were analyzed by enzyme-linked immunosorbent assay ( ELISA) . Results The expression of endostatin in serum was (131. 71 ±50. 32) ng/ ml and in BALF was (502. 56 ±302. 00)ng/ ml in patient s with lung cancer. And the expression of endostatin in patient s with benign pulmonary diseases was (85. 86 ±36. 86) ng/ ml and (279. 49 ±135. 17) ng/ ml, respectively ( P < 0. 01), there was significantly difference between them. The levels of endostatin in serum and BALF in advanced stage lung cancer patient s were higher than those of early stage patients. In adenocarcinoma patients was significantly higher than that in patients with squamous cell carcinoma and SCLC in serum and BAL F. Endostatin concent rations in BALF and in serum were significantly higher in patients with lymph node and distant metastasis than those patient s with no metastasis. The expression of endostatin in serum was closely related to that in BAL F in patients with lung cancer ( P = 0. 000) . Conclusion The detection of endostatin in serum and in BALF may be helpful to distinguish the lung cancer from benign pulmonary diseases and to reveal the biological behaviors of lung cancer well.
2007, 34(02): 115-117.
DOI: 10.3971/j.issn.1000-8578.578
Abstract：
Objective To study the cytokines production of spleen cells induced in vitro by murine 4-1BBL gene transfected Hepa1-6. Methods Murine hepatocellular carcinoma cell line Hepa1-6-m4-1BBL which could highly express murine 4-1BBL was cultivated previously. The tumor cell vaccine ( TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC) . Cocultivation TCV with syngeneic murine spleen cells and the supernatant s were harvested for detecting the cytokines ( IL-2, TNF-α and GM-CSF) . Results Comparing with TCV-Hepa1-6, high levels of IL-2, TNF-α and GM-CSF were released by the spleen cells after stimulated by TCV-m4-1BBL. Conclusion Stimulation signals delivered by 4-BBL remarkably increased the production of IL-2, TNF-α and GM-CSF.
Objective To study the cytokines production of spleen cells induced in vitro by murine 4-1BBL gene transfected Hepa1-6. Methods Murine hepatocellular carcinoma cell line Hepa1-6-m4-1BBL which could highly express murine 4-1BBL was cultivated previously. The tumor cell vaccine ( TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC) . Cocultivation TCV with syngeneic murine spleen cells and the supernatant s were harvested for detecting the cytokines ( IL-2, TNF-α and GM-CSF) . Results Comparing with TCV-Hepa1-6, high levels of IL-2, TNF-α and GM-CSF were released by the spleen cells after stimulated by TCV-m4-1BBL. Conclusion Stimulation signals delivered by 4-BBL remarkably increased the production of IL-2, TNF-α and GM-CSF.
2007, 34(02): 118-120.
DOI: 10.3971/j.issn.1000-8578.2410
Abstract：
Objective To investigate the expression of Cks1 protein and its relationship with the expression of Skp2, p27 Kip1 and tumor aggressiveness. Methods Flow cytomet ry was used to detect the expression of the three proteins in 20 cases of normal gast ric tissue and 64 cases of primary gastric cancers. Results The expression of Cks1 and Skp2 in gastric cancers was higher than that in normal gast ric tissue (χ2 = 22. 69, Ρ< 0. 05 ;χ2 = 13. 42, Ρ< 0. 05 respectively) . While the expression of p27 Kip1 was lower than that in normal gastric tissue (χ2 = 14. 83, P < 0. 05) . A significant inverse relation was observed between the expression of Cks1 or Skp2 and of p27 Kip1 ( r = - 0. 649, P < 0. 05 ; r = - 0. 732, P < 0. 05, respectively) . While there was no correlation between the expression of Cks1 and Skp2 ( P > 0. 05) . The expression of Cks1 was significantly associated with tumor differentiation. (χ2 = 5. 05, Ρ< 0. 05) . While it was not associated with depth of invasion, lymph node metasis and clinical stages ( P > 0. 05) . Conclusion Cks1 may play an important role in human gast ric tumorgenesis and it may be an essential accessory factor for Skp2 in protease-dependent dest ruction of p27 Kip1.
Objective To investigate the expression of Cks1 protein and its relationship with the expression of Skp2, p27 Kip1 and tumor aggressiveness. Methods Flow cytomet ry was used to detect the expression of the three proteins in 20 cases of normal gast ric tissue and 64 cases of primary gastric cancers. Results The expression of Cks1 and Skp2 in gastric cancers was higher than that in normal gast ric tissue (χ2 = 22. 69, Ρ< 0. 05 ;χ2 = 13. 42, Ρ< 0. 05 respectively) . While the expression of p27 Kip1 was lower than that in normal gastric tissue (χ2 = 14. 83, P < 0. 05) . A significant inverse relation was observed between the expression of Cks1 or Skp2 and of p27 Kip1 ( r = - 0. 649, P < 0. 05 ; r = - 0. 732, P < 0. 05, respectively) . While there was no correlation between the expression of Cks1 and Skp2 ( P > 0. 05) . The expression of Cks1 was significantly associated with tumor differentiation. (χ2 = 5. 05, Ρ< 0. 05) . While it was not associated with depth of invasion, lymph node metasis and clinical stages ( P > 0. 05) . Conclusion Cks1 may play an important role in human gast ric tumorgenesis and it may be an essential accessory factor for Skp2 in protease-dependent dest ruction of p27 Kip1.
2007, 34(02): 121-124.
DOI: 10.3971/j.issn.1000-8578.584
Abstract：
Objective To study the effects of the OPCML in ovarian cancer cell lines. Methods The OPCML was t ransferred to the human ovarian cancer cell lines A2780 and OCC1 in addition to normal CD1 mouse ovarian surface epithelial cells by recombinant lentiviruses which carrying OPCML, the infected cells were then characterized by cell proliferation assays, cell aggregation assays, cell cycle analysis by flow cytometry( FCM) and tumorigenicity assays following injection into nude mice. Results (1) The efficiency of infection of the cell lines using the lentiviral vectors was almost 100 % allowing the stable expression of OPCML in nearly all cells. Stable expression of OPCML (60kDa) and GFP(27kDa) proteins was confirmed by Western blot analysis. (2) A2780 cells expressing OPCML grew slowly compared to A2780 parental or control virus infected cells ( P < 0. 01), but the expression of OPCML had no effect on the proliferation rates of OCC1 and the normal CD1 cells when compared to their respective parental or controls ( P > 0. 05) . (3) Flow cytometry based cell cycle assays showed that the expression of OPCML can arrest A2780 cells ( P < 0. 05) ; but not in OCC1, CD1 cells. (4) Cell aggregation assays indicated the OPCML can increase cell surface adhesion mediated. (5) A2780 cells expressing OPCML only formed a single tumor in mice and which was significantly smaller than cont rols (4/ 4) ( P < 0. 001) . Expression of OPCML in tumor was confirmed by immunohistochemisty. Conclusion The use of lentiviral vectors allowed the efficient expression of OPCML in nearly 100 % of target cells. Expression of the OPCML resulted in an increase of cell adhesion in all cell lines tested, and decreased the proliferation and tumorigenicity of the A2780 ovarian cancer cell line. It suggest s that the OPCML maybe a new tumor suppressor gene.
Objective To study the effects of the OPCML in ovarian cancer cell lines. Methods The OPCML was t ransferred to the human ovarian cancer cell lines A2780 and OCC1 in addition to normal CD1 mouse ovarian surface epithelial cells by recombinant lentiviruses which carrying OPCML, the infected cells were then characterized by cell proliferation assays, cell aggregation assays, cell cycle analysis by flow cytometry( FCM) and tumorigenicity assays following injection into nude mice. Results (1) The efficiency of infection of the cell lines using the lentiviral vectors was almost 100 % allowing the stable expression of OPCML in nearly all cells. Stable expression of OPCML (60kDa) and GFP(27kDa) proteins was confirmed by Western blot analysis. (2) A2780 cells expressing OPCML grew slowly compared to A2780 parental or control virus infected cells ( P < 0. 01), but the expression of OPCML had no effect on the proliferation rates of OCC1 and the normal CD1 cells when compared to their respective parental or controls ( P > 0. 05) . (3) Flow cytometry based cell cycle assays showed that the expression of OPCML can arrest A2780 cells ( P < 0. 05) ; but not in OCC1, CD1 cells. (4) Cell aggregation assays indicated the OPCML can increase cell surface adhesion mediated. (5) A2780 cells expressing OPCML only formed a single tumor in mice and which was significantly smaller than cont rols (4/ 4) ( P < 0. 001) . Expression of OPCML in tumor was confirmed by immunohistochemisty. Conclusion The use of lentiviral vectors allowed the efficient expression of OPCML in nearly 100 % of target cells. Expression of the OPCML resulted in an increase of cell adhesion in all cell lines tested, and decreased the proliferation and tumorigenicity of the A2780 ovarian cancer cell line. It suggest s that the OPCML maybe a new tumor suppressor gene.
Expression of CD105, COX-2 and VEGF and Their Relationship with Angiogenesis in Colorectal Carcinoma
2007, 34(02): 125-127.
DOI: 10.3971/j.issn.1000-8578.585
Abstract：
Objective To investigate the expression CD105 in newborn vessel of COX-2 and VEGF in colorectal carcinoma and their relationship with angiogenesis. Methods Immunohisto-chemical staining (S-P method) for CD105, COX-2 and VEGF was performed in 58 cases of colorectal carcinoma ; the microvessel density (MVD) was marked by CD105. Results The value of MVD with marked CD105 was (36. 50 ±9. 59), the positive rate of COX-2 and VEGF were respectively 69 % and 67. 2 %. The MVD in COX-2 positive group or VEGF positive group was significantly higher than that in COX-2 negative group or VEGF negative group ( P < 0. 01) . The MVD in the group of both COX-2 and VEGF positive was significantly higher than that in both negative groups. There was a significant positive correlation between the expression of COX-2 and VEGF. Conclusion CD105 was a specific marker of newborn vessel in colorectal carcinoma ; COX-2 was correlated to angiogenesis and VEGF may be an important mediator in this process.
Objective To investigate the expression CD105 in newborn vessel of COX-2 and VEGF in colorectal carcinoma and their relationship with angiogenesis. Methods Immunohisto-chemical staining (S-P method) for CD105, COX-2 and VEGF was performed in 58 cases of colorectal carcinoma ; the microvessel density (MVD) was marked by CD105. Results The value of MVD with marked CD105 was (36. 50 ±9. 59), the positive rate of COX-2 and VEGF were respectively 69 % and 67. 2 %. The MVD in COX-2 positive group or VEGF positive group was significantly higher than that in COX-2 negative group or VEGF negative group ( P < 0. 01) . The MVD in the group of both COX-2 and VEGF positive was significantly higher than that in both negative groups. There was a significant positive correlation between the expression of COX-2 and VEGF. Conclusion CD105 was a specific marker of newborn vessel in colorectal carcinoma ; COX-2 was correlated to angiogenesis and VEGF may be an important mediator in this process.
2007, 34(02): 128-131.
DOI: 10.3971/j.issn.1000-8578.1535
Abstract：
Objective To investigate the effect of the short hairpin RNA ( shRNA) against human telomerase reverse t ranscriptase (hTERT) on the proliferation and apoptosis of prostate cancer cells PC-3m in vitro. Methods The recombinant plasmid psilencer-TRT was transfected into prostate cancer cell line PC-3m via liposome reagent . The level of hTERT mRNA was examined by reverse t ranscription polymerase chain reaction (RT-PCR) . The expressions of hTERT and c-myc protein were detected by western blot analysis. The effect of hTERT shRNA on the cellular proliferation capacity of PC-3m cells was assayed by the growth curve. The cell apoptosis was detected by Hoechst33258 staining, elect ron microscope, and flow cytomet ry analysis. Results The vector-mediated shRNA significantly reduced the level of hTERT mRNA by 89. 02 % af ter psilencer-TRT transduction in PC-3m cells. Meanwhile, the levels of hTERT and c-myc protein were also decreased in transfected cells. The cell proliferation was markedly inhibited compared with the cont rol cells. Partial cancer cells presented morphological changes of apoptosis, and the apoptosis rate was (19. 69 ±4. 75) %. Conclusion hTERT shRNA can suppress hTERT expression and cell proliferation, in addition to acceleration of apoptosis. This implied the possibility of RNA interfering to hTERT as the potential method for gene therapy of prostate cancer.
Objective To investigate the effect of the short hairpin RNA ( shRNA) against human telomerase reverse t ranscriptase (hTERT) on the proliferation and apoptosis of prostate cancer cells PC-3m in vitro. Methods The recombinant plasmid psilencer-TRT was transfected into prostate cancer cell line PC-3m via liposome reagent . The level of hTERT mRNA was examined by reverse t ranscription polymerase chain reaction (RT-PCR) . The expressions of hTERT and c-myc protein were detected by western blot analysis. The effect of hTERT shRNA on the cellular proliferation capacity of PC-3m cells was assayed by the growth curve. The cell apoptosis was detected by Hoechst33258 staining, elect ron microscope, and flow cytomet ry analysis. Results The vector-mediated shRNA significantly reduced the level of hTERT mRNA by 89. 02 % af ter psilencer-TRT transduction in PC-3m cells. Meanwhile, the levels of hTERT and c-myc protein were also decreased in transfected cells. The cell proliferation was markedly inhibited compared with the cont rol cells. Partial cancer cells presented morphological changes of apoptosis, and the apoptosis rate was (19. 69 ±4. 75) %. Conclusion hTERT shRNA can suppress hTERT expression and cell proliferation, in addition to acceleration of apoptosis. This implied the possibility of RNA interfering to hTERT as the potential method for gene therapy of prostate cancer.
2007, 34(02): 132-134.
DOI: 10.3971/j.issn.1000-8578.580
Abstract：
Objective To investigate the effect of curcumin on express of regulation p300 and mechanism of anticancer in vitro. Methods The bladder T24 cell was treated with curcumin in different concentration. The cell growth inhibition was determined with MTT, the level of p300 、c-fos、c-jun mRNA were assayed by reverse t ranscriptase polymerase chain reaction (RT-PCR), and the expression of acetyl histone H3 and H4 、p300 、c-fos、c-jun protein were evaluated by western blot . Results Curcumin could significantly inhibit proliferation of T24 cell in a dose and time-dependent manner. The expression of p300 、c-fos、c-jun mRNA and protein was totally decreased with increasing concent rations of curcumin. Curcumin can inhibit the expression of acetyl histone H3 and H4 protein in a dose-dependent manner. Conclusion Curcumin can inhibit the expression of p300, which may be it s molecular mechanism of anticancer.
Objective To investigate the effect of curcumin on express of regulation p300 and mechanism of anticancer in vitro. Methods The bladder T24 cell was treated with curcumin in different concentration. The cell growth inhibition was determined with MTT, the level of p300 、c-fos、c-jun mRNA were assayed by reverse t ranscriptase polymerase chain reaction (RT-PCR), and the expression of acetyl histone H3 and H4 、p300 、c-fos、c-jun protein were evaluated by western blot . Results Curcumin could significantly inhibit proliferation of T24 cell in a dose and time-dependent manner. The expression of p300 、c-fos、c-jun mRNA and protein was totally decreased with increasing concent rations of curcumin. Curcumin can inhibit the expression of acetyl histone H3 and H4 protein in a dose-dependent manner. Conclusion Curcumin can inhibit the expression of p300, which may be it s molecular mechanism of anticancer.
2007, 34(02): 135-136.
DOI: 10.3971/j.issn.1000-8578.581
Abstract：
Objective To study on the count s of MC and TAM and detect their clinicopathologic significances and relationship in human laryngneal squamous cell carcinoma tissues. Methods SP immunohistochemical method was used for the count s of MC and TAM on the routinely paraffin-embedded sections of surgical operated specimens of 41 cases with laryngneal squamous cell carcinoma. Results The count mean of MC and TAM was 19. 73 ±5. 50/ HP and 25. 95 ±7. 39/ HP, respectively. The count means of MC and TAM were significantly lower in the cases of aged ≤60 years, histologic grade I, without-metastasis of lymph node, and clinical stage T1 than that in the ones of aged > 60 years, histologic grade III, with-metastasis of lymph node and clinical stage T3 ( P < 0. 05 or P < 0. 01) . The close positive correlation was found between the count of MC and TAM. Conclusion The count of MC or/ and TAM might be important factor in reflecting the progression, biological behaviors and prognosis evaluating of the laryngneal squmous cell carcinoma.
Objective To study on the count s of MC and TAM and detect their clinicopathologic significances and relationship in human laryngneal squamous cell carcinoma tissues. Methods SP immunohistochemical method was used for the count s of MC and TAM on the routinely paraffin-embedded sections of surgical operated specimens of 41 cases with laryngneal squamous cell carcinoma. Results The count mean of MC and TAM was 19. 73 ±5. 50/ HP and 25. 95 ±7. 39/ HP, respectively. The count means of MC and TAM were significantly lower in the cases of aged ≤60 years, histologic grade I, without-metastasis of lymph node, and clinical stage T1 than that in the ones of aged > 60 years, histologic grade III, with-metastasis of lymph node and clinical stage T3 ( P < 0. 05 or P < 0. 01) . The close positive correlation was found between the count of MC and TAM. Conclusion The count of MC or/ and TAM might be important factor in reflecting the progression, biological behaviors and prognosis evaluating of the laryngneal squmous cell carcinoma.
2007, 34(02): 137-139.
DOI: 10.3971/j.issn.1000-8578.1544
Abstract：
Objective The purpose of this paper was to discuss the sensitivity of Hodgkin' s lymphoma ( HL) and non-Hodgkin's lymphoma (NHL) in fine needle aspiration cytopathology and to discuss erroneous diagnosis which is regularly met in clinical cytology. Methods To compare 318 lymphoma cases' cytopathology with histopathology. Results The sensitivity of lymphoma is 95. 6 % and that of HL and NHL are 93. 7 %, 96. 1 % respectively. Of the 69 HL cases, 3 cases were incorrectly diagnosed as NHL and 1 case was incorrectly diagnosed as reactive hyperplasia. We found that NHL was usually confused with small-cell tumors such as small-cell cancer, non-typical carcinoid, embryonal rhabdomyosarcoma etc. Of the 249 NHL cases, 6 were incorrectly diagnosed as HL, including two dispersed large-cell B, two T cell-rich B cell lymphoma, and one follicular lymphoma, one anaplastic large cell lymphoma. The result s of immunocytochemistry and histopathology are consistent . Conclusion Fine needle aspiration cytopathology may work well to precisely diagnose lymphoma. In order to obtain satisfied samples, it is highly recommended that these samples should be aspirated at multiple locations and make best use of other clinical materials. Immunocytochemistry can become a auxiliary method in cytopathology diagnosis.
Objective The purpose of this paper was to discuss the sensitivity of Hodgkin' s lymphoma ( HL) and non-Hodgkin's lymphoma (NHL) in fine needle aspiration cytopathology and to discuss erroneous diagnosis which is regularly met in clinical cytology. Methods To compare 318 lymphoma cases' cytopathology with histopathology. Results The sensitivity of lymphoma is 95. 6 % and that of HL and NHL are 93. 7 %, 96. 1 % respectively. Of the 69 HL cases, 3 cases were incorrectly diagnosed as NHL and 1 case was incorrectly diagnosed as reactive hyperplasia. We found that NHL was usually confused with small-cell tumors such as small-cell cancer, non-typical carcinoid, embryonal rhabdomyosarcoma etc. Of the 249 NHL cases, 6 were incorrectly diagnosed as HL, including two dispersed large-cell B, two T cell-rich B cell lymphoma, and one follicular lymphoma, one anaplastic large cell lymphoma. The result s of immunocytochemistry and histopathology are consistent . Conclusion Fine needle aspiration cytopathology may work well to precisely diagnose lymphoma. In order to obtain satisfied samples, it is highly recommended that these samples should be aspirated at multiple locations and make best use of other clinical materials. Immunocytochemistry can become a auxiliary method in cytopathology diagnosis.
2007, 34(02): 140-142.
DOI: 10.3971/j.issn.1000-8578.1545
Abstract：
Objective To study the clinical and pathological characteristics, subtypes and immunophenotypes of nodular fasciitis. Methods The histopathological features of 35 cases of nodular fasciitis were observed using light microscopy and immunochemistry, then clinical data was analyzed and the literature was reviews. Results Nodular fasciitis (NDF) occurs in all age groups but more often in young adults of 20 to 40 years old. The lesion is usually any part of the body's subcutaneous and of ten locates in upper extremity and trunk, clinically, NDF typically grows rapidly and of ten small sizes. Histopathologically, it is composed of plump fibroblasts or myofibroblasts, which of ten grow in S-shaped, fascicles or semicircinate pattern, lacking pleomorphic cells and easy to see mitoses. The lesion has loose and myxoid stroma, abundant vessels, ext ravasated red cells and irregular cranny, which is important features conduced to diagnose. Histologicly, there are 4 subtypes : myxoid NDF(10 cases), granulomatous NDF(15cases), giant cell NDF(2 cases) and fibromatous NDF(8 cases), which represent the disease's early, middle and later stages changes respectively. Immunophenotype demonst rates the vimentin, SMA and MSA are positive in tumor cells but S-100 and CD34 are negative. Conclusion NDF is a complex lesion, in which fibroblasts or myofibroblast s are hyperplasia and its component s are multiplex. So mastering the lesion's clinical features, histopathological configuration and immunophenotype can prevent the mistakes in diagnosis of sarcoma.
Objective To study the clinical and pathological characteristics, subtypes and immunophenotypes of nodular fasciitis. Methods The histopathological features of 35 cases of nodular fasciitis were observed using light microscopy and immunochemistry, then clinical data was analyzed and the literature was reviews. Results Nodular fasciitis (NDF) occurs in all age groups but more often in young adults of 20 to 40 years old. The lesion is usually any part of the body's subcutaneous and of ten locates in upper extremity and trunk, clinically, NDF typically grows rapidly and of ten small sizes. Histopathologically, it is composed of plump fibroblasts or myofibroblasts, which of ten grow in S-shaped, fascicles or semicircinate pattern, lacking pleomorphic cells and easy to see mitoses. The lesion has loose and myxoid stroma, abundant vessels, ext ravasated red cells and irregular cranny, which is important features conduced to diagnose. Histologicly, there are 4 subtypes : myxoid NDF(10 cases), granulomatous NDF(15cases), giant cell NDF(2 cases) and fibromatous NDF(8 cases), which represent the disease's early, middle and later stages changes respectively. Immunophenotype demonst rates the vimentin, SMA and MSA are positive in tumor cells but S-100 and CD34 are negative. Conclusion NDF is a complex lesion, in which fibroblasts or myofibroblast s are hyperplasia and its component s are multiplex. So mastering the lesion's clinical features, histopathological configuration and immunophenotype can prevent the mistakes in diagnosis of sarcoma.
2007, 34(02): 143-145.
DOI: 10.3971/j.issn.1000-8578.3319
Abstract：
Objective To probe the relation ship between lymph node metastasis rate and various kinds pathologic appearance of esophageal carcinoma. Methods For the 149 esophageal carcinoma cancer patients who were diagnosed, their clinical data were analyzed ret rospectively. Results All of lymph node detected were 765, the metastatic were 336 and the metastasis rate was 43. 9 %. The lymph node metastasis rate of various group were clavicle regional > mediastinal > arteriae gast rica sinist ra regional > esophagus side in order. The difference between the deep of tumor infilt ration, the degree of cell differentiation and lymph node metastasis rate were significance. While the difference between the lesion site, lesion length and lymph node metastasis rate were not significance. Conclusion The lymph node metastasis rate of lengthways of esophageal carcinoma was greater than the transversal. The esophagus side lymph node could not be regarded as sentinel node of esophageal cancer. For decreasing residual of lymph node and improving prognosis of patients, extensive cervical part, thoracic part and abdominal part lymph node scavenge should be carried out .
Objective To probe the relation ship between lymph node metastasis rate and various kinds pathologic appearance of esophageal carcinoma. Methods For the 149 esophageal carcinoma cancer patients who were diagnosed, their clinical data were analyzed ret rospectively. Results All of lymph node detected were 765, the metastatic were 336 and the metastasis rate was 43. 9 %. The lymph node metastasis rate of various group were clavicle regional > mediastinal > arteriae gast rica sinist ra regional > esophagus side in order. The difference between the deep of tumor infilt ration, the degree of cell differentiation and lymph node metastasis rate were significance. While the difference between the lesion site, lesion length and lymph node metastasis rate were not significance. Conclusion The lymph node metastasis rate of lengthways of esophageal carcinoma was greater than the transversal. The esophagus side lymph node could not be regarded as sentinel node of esophageal cancer. For decreasing residual of lymph node and improving prognosis of patients, extensive cervical part, thoracic part and abdominal part lymph node scavenge should be carried out .
2007, 34(02): 146-148.
DOI: 10.3971/j.issn.1000-8578.1546
Abstract：
Objective To investigate the safety, feasibility and adverse reaction of combination of G-CSF and GM-CSF in patient s with Ⅱdegree and above arrest of bone marrow caused by chemotherapy. Methods 84 patients with definite malignant tumor and Ⅱdegree and above arrest of bone marrow caused by chemotherapy were enrolled into a single-blinded and random study : G-CSF group ( G group, n = 28, G-CSF 150μg/ d subcutaneous injection), GM-CSF : group ( GM group : n = 28, GM-CSF 150 μg/ d subcutaneous injection), combination drugs group ( G/ GM group, n = 28, GM-CSF 75 μg/ d and GM-CSF 75 μg/ d subcutaneous injection) . Observation items : (1) the days of WBC normalization af ter CSF application ;(2) WBC count after 48 hours withdrawal of CSF ; (3) PL T count af ter 48 hours withdrawal of CSF ; (4) incidence rate of adverse reaction. Results Leukcocyte count in three groups increased. The days of WBC normalization in GM group were longer than that in G group and G/ GM group ( P < 0. 05) . WBC count af ter 48 hours withdrawal of CSF in GM group and G/ GM group was obviously more than that in G group ( P < 0. 05) . Incidence rate of adverse reaction in three groups was approximate and there was no significant difference between groups. Conclusion Combination of G-CSF and GM-CSF was an effective and safe treatment to be applied in patient s with arrest of bone marrow caused by chemotherapy, and it could reduce the defect of single application.
Objective To investigate the safety, feasibility and adverse reaction of combination of G-CSF and GM-CSF in patient s with Ⅱdegree and above arrest of bone marrow caused by chemotherapy. Methods 84 patients with definite malignant tumor and Ⅱdegree and above arrest of bone marrow caused by chemotherapy were enrolled into a single-blinded and random study : G-CSF group ( G group, n = 28, G-CSF 150μg/ d subcutaneous injection), GM-CSF : group ( GM group : n = 28, GM-CSF 150 μg/ d subcutaneous injection), combination drugs group ( G/ GM group, n = 28, GM-CSF 75 μg/ d and GM-CSF 75 μg/ d subcutaneous injection) . Observation items : (1) the days of WBC normalization af ter CSF application ;(2) WBC count after 48 hours withdrawal of CSF ; (3) PL T count af ter 48 hours withdrawal of CSF ; (4) incidence rate of adverse reaction. Results Leukcocyte count in three groups increased. The days of WBC normalization in GM group were longer than that in G group and G/ GM group ( P < 0. 05) . WBC count af ter 48 hours withdrawal of CSF in GM group and G/ GM group was obviously more than that in G group ( P < 0. 05) . Incidence rate of adverse reaction in three groups was approximate and there was no significant difference between groups. Conclusion Combination of G-CSF and GM-CSF was an effective and safe treatment to be applied in patient s with arrest of bone marrow caused by chemotherapy, and it could reduce the defect of single application.
2007, 34(02): 149-150.
DOI: 10.3971/j.issn.1000-8578.1547
Abstract：
Objective To investigate the effects and complications for treatment of malignant biliary obstruction by implantation stend. Methods 46 patients with malignant biliary obstruction were received implantation stand through the guidance of DSA. Results 46 patients were inserted biliary stand, and used the total 50 stents, including patient s with biliary double sand. 4 patients were performed with TAI or / and TAE after operation. The total efficacy of alleviation jaundice was 85. 4 %. 3 cases of stand were obstruction. Complications included septicemia ( n = 3), hepatic dysfunction ( n = 6) . Conclusion The implantation stand has high successful rate, the better effects of alleviation jaundice. And it is a simple, safe of palliative treatment for malignant biliary obstruction.
Objective To investigate the effects and complications for treatment of malignant biliary obstruction by implantation stend. Methods 46 patients with malignant biliary obstruction were received implantation stand through the guidance of DSA. Results 46 patients were inserted biliary stand, and used the total 50 stents, including patient s with biliary double sand. 4 patients were performed with TAI or / and TAE after operation. The total efficacy of alleviation jaundice was 85. 4 %. 3 cases of stand were obstruction. Complications included septicemia ( n = 3), hepatic dysfunction ( n = 6) . Conclusion The implantation stand has high successful rate, the better effects of alleviation jaundice. And it is a simple, safe of palliative treatment for malignant biliary obstruction.
