细胞核DNA含量与神经系统良恶性肿瘤关系的研究

细胞核DNA含量与神经系统良恶性肿瘤关系的研究

Study on the correlations of cellular DNA content with benign and malignant tumors of central nervous system

摘要

摘要: 目的：探讨DNA含量定量分析在中枢神经良、恶性肿瘤的判定及星形细胞瘤分级诊断中的应用价值。方法：运用计算机辅助图象分析并结合组织细胞化学技术测定肿瘤细胞核的DNA含量及DNA倍体细胞分布情况。结果：星形细胞瘤按WHO分级标准分为Ⅰ～Ⅳ级，其DNA相对含量和DNA指数分别为Ⅰ级4.11±0.75，1.44±0.28；Ⅱ级4.52±1.20，1.71±0.34；Ⅲ级6.35±1.82，2.54±0.44；Ⅳ级7.07±1.32，3.13±0.59。髓母细胞瘤与听神经瘤与DNA相对含量和DNA指数分别为5.61±2.10，2.33±0.83；3.18±0.94，1.35±0.26，方差分析显示组间存在显著差异（P<0.01），运用Q值法两两比较，髓母细胞瘤与Ⅲ级星形细胞瘤组间、听神经瘤与Ⅰ级星形细胞瘤组间、Ⅰ级星形细胞瘤与Ⅱ级星形细胞瘤组间均无显著差异（P均＞0.05），余各组间均差异显著（P均<0.01）。结论：DNA含量测定是判定中枢神经肿瘤良、恶性程度及辅助星形细胞瘤分级诊断的良好手段。

Abstract: Objective To explore the significance of cellular DNA content analysis in diagnosis of histological grades of benign and malignant tumor of central nervous system. Methods The cellular DNA content and DNA ploidy of tumors were measured by quantitative imageanalysis with histochemistry techniques. Results The DNA content and DNA Indes (DI) in astrocytoma were that: gradeⅠ4.11±0.75，1.44±0.28；gradeⅡ 4.52±1.20，1.71±0.34；gradeⅢ6.35±1.82，2.54±0.44；gradeⅣ7.07±1.32，3.13±0.59。) medulloblastoma 5.61±2.10，2.33±0.83；3.18±0.94，1.35±0.26， There were no remarkable difference between medulloblastoma and grade Ⅲ astrocytoma, acorstic neurioma and grade I astrocytoma, grade I and grade Ⅱ astrocytoma groups. But between other groups, there were remarkable difference (P<0.01). Conclusions Cellular DAN content analysis is a useful way to make histological diagnosis of benign and malignant tumor of central nervous system.

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