Abstract: To study of the relation between metastasis and the expression of nm₂₃-H₁,PCNA,CD15 in human lung carcinoma. The expression of nm₂₃-H₁,PCNA, CD15in 47 cases human lung carcinoma was studied with SPimmunohistochemical technique. In human lung carcinoma expressions of nm₂₃-H₁,PCNA, CD15were 51. 6 %, 65. 96 %and 51. 06%. The positive rate of nm₂₃-H₁ in squamous cell carcinoma was 43. 33%, adenocarcinoma 64.7%. The positive rate in the adenocarcinoma was higher than the squamous cell carcinoma. The positive rate in squamors cell carcinoma without metastasis of hilar or mediastinal lymph node was 62. 5 % (10/16), compared with these with metastasis of lymph node was 21. 4 % (3/14), (P 0. 001 ). The level of nm₂₃-H₁ expression was inversely correlated with lymph node metastasis in squamous cell carcinoma. The positivity of PCNA in the cases with lymph node metastasis was 82. 6% (19/23) and those without metastasis was 54. 16% (P0. 001). The rate of positivity of PCNA in the poorly differentiated carcinoma was 88. 88% (8/9) and cases of with the well differentiatde carcinoma was 43. 75% (7/16) (P0. 001 ). The rate of positivity of CD15in cases of with lymph node metastasis was 78. 26% (18/23) and in those without metastasis was 25%(P0. 001). The expression of nm₂₃-H₁,PCNA and CD15may be helpful to evaluate the prognosis of lung carcinoma.