人小细胞肺癌P<sup>53</sup>基因突变的研究

人小细胞肺癌P⁵³基因突变的研究

摘要: 为探讨小细胞肺癌（SCLC）发生的分子机制，应用免疫组化和聚合酶链反应─-单链构象多态性（PCR－SSCP）技术，对14例SCLC的石蜡切片标本进行了P⁵³基因突变研究。结果显示：P⁵³蛋白免疫组织化学反应有9例出现阳性，其中2例为十，7例为～，阳性率为64.3%（9/14）。P⁵³基因第5、6、7、8外显子突变率分别为21.4%（3/14）；14，3%（2/14）;14.3%（2/14）；7.1%（1/14），总突变率为57.1%（8/14）。提示：SCLC中存在较高的p⁵³基因突变率，P⁵³基因突变在人类肺癌的发生发展过程中起着重要的作用。

Abstract: To discuss the molecuar mechanism of the small cell lung cancer (SCLC). P⁵³ gene mutation in SCLC of 14 paraffin embedded tissue section was observed by immunohistochemical staining (LSAB method) and polymerase chain reaction and single-strand conformation polymorsphism (pcr-SSCP) analysis. The results showed that the positive rate of immunohistochemical reaction was 64. 3% (9/14). of which 2 cases are, 7 cases are ～。 P⁵³ gene point mutation in exon 5, 6, 7 and 8 are respectively: 21. 4% (3/14) ；14. 3% (2/14)；14. 3% (2/14) ；7. 1 % (1/14). The total mutation rate is 57. 1 % (8/14). It was suggested that Comparatively high mutation rate of P⁵³ gene exists in SCLC,which might be the crucial gene of cause of the human lung carcinoma.