Abstract: The amounts of urinary polyamines of 32 patients with lymphosacoma were determined. The urinary polyamines in these patients were significantly higher than those in normal subjects. The putrescine, spermidine and spermine had respectively increases by 1.52 times, 0.58 times and 4.52 times as compared with those in normal subjects.Of the patients, 100% had a rise in one of the three polyamines, while 81.25% in two. Between the putrescine and spermidine in the electrophoregram a histamines'like substance was detected. Whose value was also high, and 1.55 times higher than the normal value, with a positive elevated rate of 37.5%, The assay of urinary polyamines can be used as an auxiliary method in the diagnosis of lymphosacoma.