二亚硝基哌嗪诱发移植于裸鼠的人胚鼻咽粘膜癌变的研究

Carcinogenesis of DNP on I-Iuman Embro Nasopharyngeal Mucosa Transplants in Nude Mice

摘要: 人胚弄咽粘膜移植于裸鼠皮下后可长期存活，并保持其结构和功能，用二亚梢基哌嗪(DNP)皮下给药法诱发其癌变.实验分四组，一次性给药组(11例)，低剂量多次给药组(12例)，大剂量多次给药组(12例)，大剂量DNP+促法剂PMA组(11例).在40周内，分别见不典型性增生及异型鲜状化生5、 6、6及7例次，原位法依次为3、1、 1及1例，而大荆蚤DNP+PMA组及低荆黄多次给药组各诱发浸润癌一例.

Abstract: Human embro nasopharyngeal mucosa tissues which had been subcutaneously transplanted into nude mice remained their primary structure and function. Based on this experimental model, N'Ndinitrosopiperazine (DNP) was used to induce the transplant mucosa epithelia into cancerous lesion.The experiments had four groups:once DNP administered group (11 case); low dose serial DNP administered group (12 cases); large dose serial DNP administered group (12 case); large dose serial DNP and PEA administered group (11 cases). The result showed that within 40 weeks, there were atypical hyperplasia (5, 6, 5, 4 cases separately); carcinoma in situ (3, 1, 1, 1 cases separately); infiltrating carcinoma (1 case in large dose serial DNP and PMA administered group).