钳蝎蝎毒组分Ⅱ对人直肠腺癌C1184细胞生长的影响

钳蝎蝎毒组分Ⅱ对人直肠腺癌C1184细胞生长的影响

The effect of the component Ⅱ in scorpion venom crude extracted from buthus martensii karsch(svc Ⅱ)on the human rectum adenocarcinoma C1184 cell line

摘要

摘要: 蝎毒组分Ⅱ(ScorpionVenomcrudeⅡ，SVCⅡ)为河南马氏钳蝎粗毒(SVC)经分离纯化后获得的有效成分之一。实验结果表明，以丝裂霉素作为阳性对照，SVCⅡ(4～20μg/ml)对人直肠腺癌C1184细胞株有明显杀伤作用。与空白对照相比，SVCⅡ使细胞生长抑制率、细胞毒性和克隆形成抑制率增大(P<0.05或(P<0.01)，并存在剂量效应关系，其半数抑制浓度分别为11.20μg/ml、11.93μg/ml和17.38μg/ml。从生长曲线可见，SVCⅡ对细胞生长呈剂量和时间依赖性抑制。SVCⅡ还能使细胞分裂指数下降。提示SVCⅡ具有抗癌细胞增殖作用。

Abstract: Scorpion Venom Crude Ⅱ(SVC Ⅱ)was one of the effective components which was isolated from the scorpion venom crude extracted from Buthus Martensii Karsch in Henan Province.The experimentalresults showed that SVC Ⅱ with the concentration ranging from 4μg/ml to 20μg/ml had obvious killing acticitv on the human rectum adenocarcinoma C1184 cell line in Vitro in comparison with the positive control which was Mitomycin-c.Compared with the blank control,SVC Ⅱcould induceto increase the inhibitary rate of cell growth,the cvtotoxicitv and inhibited rate of the colonv formation(P0.05 or P0.01).There existed the dose-response correlations between the inhibitioneffect and the dose of SVC Ⅱ.The half inhibition concentrations of cell growth(Ⅰ C50)in above experiments were 11.20μg/ml、11.93μg/ml and 17. 38μg/ml,respectively.It was found that SVCⅡ signficantly inhibited C1184 cell growth in the dose-dependent and time-dependent manner in cell growth curve.The mitotic index of C1184 cells could be reduced by SVC Ⅱ.So it was supported that.SVC Ⅱpossess antineoplastic effect on tumor cell proliferation.

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