肝细胞癌Rb基因缺失的研究
Studies of deletion of Rb gene in HCC
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摘要: 应用PCR－RFLP技术检测了24例肝细胞癌抑癌基因Rb杂合缺失，发现25％（3／12）的肝细胞癌存在内含子1的缺失。同时用Rb基因cDNA为探针Southern杂交检测了10例肝细胞癌，发现2例分别在4．5Kb和9．8Kb，7．5Kb处缺失。所有发生Rb基因缺失的肝癌均为进展期。该结果表明：抑癌基因Rb的缺失可能参与了肝癌的进展过程。Abstract: LOH of Rb gene at intron 1 was detected by PCR-RFLP in 24 HCCs·25%<3/12) LOH was found.At same time we also analyzed the structural alteration of the Rb gene from 10 cases by Southern hybridization using the Rb cDNA. The deletion was found at 4.5kb band in one case，at 9.8kb and 7.5kb bands in another case.All the cases with Rb gene abberation were found only in markedly advanced HCC.It suggest that deletion of Rb gene may participate in the progression of HCC.
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