肝细胞癌Rb基因缺失的研究

Studies of deletion of Rb gene in HCC

摘要: 应用PCR－RFLP技术检测了24例肝细胞癌抑癌基因Rb杂合缺失，发现25％（3／12）的肝细胞癌存在内含子1的缺失。同时用Rb基因cDNA为探针Southern杂交检测了10例肝细胞癌，发现2例分别在4．5Kb和9．8Kb，7．5Kb处缺失。所有发生Rb基因缺失的肝癌均为进展期。该结果表明：抑癌基因Rb的缺失可能参与了肝癌的进展过程。
- 肝细胞癌 /
- Rb基因 /
- 多聚酶链反应 /
- 限制性片段长度多态性 /
- Southern杂交
Abstract: LOH of Rb gene at intron 1 was detected by PCR-RFLP in 24 HCCs·25%<3/12) LOH was found.At same time we also analyzed the structural alteration of the Rb gene from 10 cases by Southern hybridization using the Rb cDNA. The deletion was found at 4.5kb band in one case，at 9.8kb and 7.5kb bands in another case.All the cases with Rb gene abberation were found only in markedly advanced HCC.It suggest that deletion of Rb gene may participate in the progression of HCC.
- Hepatocellular Carcinoma /
- Retinoblastoma gene /
- Polymerase Chain reaction /
- Restriction fregment length polymorphism /
- Southern hybridization blot

[1]	Weinberg RA,The retinoblastoma gene and gene product.Cancer survey.1992,12.43-57
[2]	Harbour TW.Yee JK ShewJY et al.abnormalities tomagene in SCLC.Science,1988.241:353-357
[3]	Lee YH TOH Shew et al inactivaion of the retinoblastoma susceptibility gene in human breast cancer.Science 1988.241:218-221
[4]	McDaniel T, Carb one D, Takahashi T, et al. PCR-based detection of a polymorphic I3amH I of the human Rbgene. Nuleic Acids Res, 1990, 18:1666。
[5]	Zhang X, xu HJ, Murakami Y, et al. Deletion of Chromosom 13q, mutation in human hepatocellularcarcinoma. Cancer Res, 1994, 54:4177^4182.
[6]	Fujimori M, Tokimo T, Hino O, et al. Alletype study of primary hepatocellular carcinoma. Cancer Res,1991. 56:89-93.
[7]	Murakami LA, Hayashi K, Hirohashi S, et al. Abberation of the tumor suppressor p53 and retinoblastoma gene in human hepatocellular carcinoma. Cancer Res,1991，51:5520-5525.
[8]	Nishida N, Fukuda Y, Kokuryu H, et al. Accmulationof allelic loss on arms of chromosome 13q, 16q and 17p in the advanced stages of human hepatocelullar carcino-ma. Int 1 Cancer, 1992, 51:862-868