刺五加多糖和GST对实验性肝癌影响的病理学研究

刺五加多糖和GST对实验性肝癌影响的病理学研究

Pathoiogic Study on effect of Acanthopanax Santicosus Polysaccharides and GST during rats hepatocarcinogenesis

摘要

摘要: 120只实验性大鼠在化学致癌物（3'-me-DAB）诱发肝癌基础上，通过对肝脏病理形态、γ-谷氨酰转肽酶（γ-GT）阳性细胞和肝组织内γ-GT含量的检测，观察利五加多糖和谷胱甘肽硫转移酶（GST）对肝细胞增生癌变过程的影响。结果表明1.刺五加组肝脏形态学与3'-me-DAB组无明显差异但肝脏发生病变的时间向后推迟了一周；肝细胞γ-GT阳性强度明显低于3'-me-DAB组；肝组织γ-GT含量动态分析发现低于3'-me-DAB组且缓慢增加，实验第9天才有升高趋势（3'-me-DAB组第4天就迅速升高）。提示剩五加多糖对肿瘤性增殖有明显延缓作用。2.GST组病理形态和3'-me-DAB组无异；肝细胞γ-GT阳性强度及府组织γ-GT含量明显增加，高于3'-me-DAB组，特别是实验后34天出现持续升高态直至实验结束。提示GST皮下免疫法未能起到保肝解毒作用。本文对利五加的研究初步表明有抑瘤作用，增加实验剂量进一步的研究是必要的。

Abstract: The effect of Acanthopanax Santicosus polysaccharides(ASPS)and Glutathione S-transferase(GST)for hepatic cellular morphology, the number of Y-GIu-tamyl transpeptiase(Y-GT) positive cells and Y-GT tissue content were studed in 148 rat livers based on 3'-me-DAB hepatocarcinogenesis. Results show:1, In APSP group, hepatomorphology are similar to 3'-me-DAB group and the number of Y-GT positive cells and Y-GT content of hepatic tissue slowly increase, with a lower proliferatic level than in 3'-me-DAB group;In Apsp group, 9 day after experimenl show pathologic Variety, but in 3'-me-DAB group 4 day;These data demonstrate ASPS have distinct retardant effect to neoplastic proliferation 2, pathomorphology in GST group are also similar with 3'-me-DAB group, and the number of Y-GT poshive cells and Y-GT content indictate higher proliferatic level than 3'-me-DAB group.It suggestes the effect of hepatic detoxication in GST is suspected by appling subcutaneous immunomethod.Influnce on experimental hepatocarcinoma in ASPS make further study, incrensing ASPS dose be suggested.

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