Experiment study of Biological action of Electrochemistry on Human cervical carcinoma Hela-S3 colon
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摘要: 本实验采用体外实验并利用MTT法和放射自影技术，观察在不同电压和电量组合的ECT作用下对人宫颈癌细胞系（Hela）的杀伤率、细胞的增殖曲线、核分裂指数以及细胞增殖周期影响，旨在了解ECT是否对Hela细胞的生物学行为有影响。其结果显示：（1）ECT对Hela细胞的增殖率有明显的抑制作用，其中以5V10C组的抑制率最为明显。（2）ECT对Hela细胞有较强的杀伤作用，而且这种杀伤作用，在电压一定时，与电量成正比。（3）ECT对Hela细胞的增殖周期有明显的影响，这对揭示ECT治癌机理和为临床选择适当有效的治疗参数均有重要的意义。Abstract: The effect of ECT combinated by different voltage and electric quantity on killer rate of Human cervical carcinoma Hela-S3 colon. proliferating rate of cells, karyokinesis inde and cells proliferating period was observed by MTT methed and autor adiography. The purpose of the experiments is to find out the effect of ECT on the bilologi-cal act of Hela-S3 colon.The results showed ECT has the following action: (1)Ob-vious inhibition action on the proliferating of Hela-S3 colon.The inhibiting rate of 5V10C is the most obvious one. (2)Killer action on Hela-S3 colon.It has direct rate with electric quatity under the same vloltage. (3)Obvious effect on the cells proliferating period, mainly on GI-S period and M period and observed, and it is re-lated with voltage,electric quantiry. The above results showed ECT has obvious effect on the bilogioal behavior. The results also contribute to the treatment of human cervical carcinoma, they play an important part when doctors are selecting effective ways of treating patients.
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Key words:
- electroehemistry /
- cervical careinoma /
- Hela cell /
- celldynamic
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[1] 唐步坚, 李力, 古明华, 等. 电化学对宫颈癌治疗的临床价值探讨. 广西医学, 1994, 16(l): 4. [2] 昊克林, 刘树林, 李亚琪, 等. 电化学治疗, 介入性化疗及放射综合治疗宫颈癌近期疗效. 肿瘤防治研究, 1994, 21(l): 48. [3] 刘鼎新主编. 放射自显影. 科学出版社, 1997: 123. [4] Nordenstrom BE.biologically clcosed electric: activa-tion of vascular interstitial closed electric circuits fortreatment of inoperable cancers. J Bioelec, 1984, 3: 137. [5] Norderstrom BE. An additional ciraulatory system:vaseular-intestitial closed electric circuits (VICC). JBoil Phys, 1987, 15: 43. [6] 李建人. 直流电对Lewis肺癌细胞杀伤作用的实验研究. 中华理疗杂志, 1992, 2(2): 74. [7] Erbe E, Vaghi M, Pepes, et al.DNA index of ovaraincarcinoma sfrom 56 Patients: in vivo in vitro stadies. Br J cancer, 1985, 52: 565.
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